Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells

The effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glu...

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Autores: López Martín, Sergio, Montserrat de la Paz, Sergio, Lucas Rodríguez, Ricardo, Bermúdez Pulgarín, Beatriz, Abia González, María del Rocío, Morales, Juan C., García Muriana, Francisco José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130762
Acceso en línea:https://hdl.handle.net/11441/130762
https://doi.org/10.1016/j.jff.2016.12.033
Access Level:acceso abierto
Palabra clave:Endothelial cells
Human
Hydroxytyrosol
Inflammation
Metabolites
Mice
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repository_id_str
spelling Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cellsLópez Martín, SergioMontserrat de la Paz, SergioLucas Rodríguez, RicardoBermúdez Pulgarín, BeatrizAbia González, María del RocíoMorales, Juan C.García Muriana, Francisco JoséEndothelial cellsHumanHydroxytyrosolInflammationMetabolitesMiceThe effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glutathione, glutathione peroxidase 1, and glutamate-cysteine ligase catalytic subunit and up-regulated heme oxygenase-1 levels. HTyr and all tested HTyr metabolites (HTyr sulfate > HTyr glucuronate > HTyr) suppressed the phosphorylation of nuclear factor kappa B proteins, the gene expression of intercellular and vascular adhesion molecules, E-selectin, chemokine (CC) motif ligand 2, and prostaglandin-endoperoxidase synthase 2 and the adhesion of human monocytes. In addition, HTyr sulfate metabolites suppressed plantar and ear swelling and myeloperoxidase activity in inflamed ear tissue in mice treated with carrageenan or 12-O-tetradecanoylphorbol-13-acetate. This study demonstrates the antioxidant and/or anti-inflammatory properties of HTyr metabolites in TNF-α-activated hECs and in the prevention of acute and chronic inflammation in mice.Junta de Andalucía 09-CVI-5007Ministerio de Ciencia e Innovación ES-2012-056104Ministerio de Economía y Competitividad JCI-2012-13084ElsevierBiología CelularBioquímica Médica y Biología Molecular e InmunologíaQuímica Orgánica y FarmacéuticaConsejo Superior de Investigaciones Científicas (CSIC)2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130762https://doi.org/10.1016/j.jff.2016.12.033reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Functional Foods, 29, 238-247.09-CVI-5007ES-2012-056104JCI-2012-13084JAEDOC089https://doi.org/10.1016/j.jff.2016.12.033info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1307622026-06-17T12:51:07Z
dc.title.none.fl_str_mv Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
title Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
spellingShingle Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
López Martín, Sergio
Endothelial cells
Human
Hydroxytyrosol
Inflammation
Metabolites
Mice
title_short Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
title_full Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
title_fullStr Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
title_full_unstemmed Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
title_sort Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
dc.creator.none.fl_str_mv López Martín, Sergio
Montserrat de la Paz, Sergio
Lucas Rodríguez, Ricardo
Bermúdez Pulgarín, Beatriz
Abia González, María del Rocío
Morales, Juan C.
García Muriana, Francisco José
author López Martín, Sergio
author_facet López Martín, Sergio
Montserrat de la Paz, Sergio
Lucas Rodríguez, Ricardo
Bermúdez Pulgarín, Beatriz
Abia González, María del Rocío
Morales, Juan C.
García Muriana, Francisco José
author_role author
author2 Montserrat de la Paz, Sergio
Lucas Rodríguez, Ricardo
Bermúdez Pulgarín, Beatriz
Abia González, María del Rocío
Morales, Juan C.
García Muriana, Francisco José
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biología Celular
Bioquímica Médica y Biología Molecular e Inmunología
Química Orgánica y Farmacéutica
Consejo Superior de Investigaciones Científicas (CSIC)
dc.subject.none.fl_str_mv Endothelial cells
Human
Hydroxytyrosol
Inflammation
Metabolites
Mice
topic Endothelial cells
Human
Hydroxytyrosol
Inflammation
Metabolites
Mice
description The effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glutathione, glutathione peroxidase 1, and glutamate-cysteine ligase catalytic subunit and up-regulated heme oxygenase-1 levels. HTyr and all tested HTyr metabolites (HTyr sulfate > HTyr glucuronate > HTyr) suppressed the phosphorylation of nuclear factor kappa B proteins, the gene expression of intercellular and vascular adhesion molecules, E-selectin, chemokine (CC) motif ligand 2, and prostaglandin-endoperoxidase synthase 2 and the adhesion of human monocytes. In addition, HTyr sulfate metabolites suppressed plantar and ear swelling and myeloperoxidase activity in inflamed ear tissue in mice treated with carrageenan or 12-O-tetradecanoylphorbol-13-acetate. This study demonstrates the antioxidant and/or anti-inflammatory properties of HTyr metabolites in TNF-α-activated hECs and in the prevention of acute and chronic inflammation in mice.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130762
https://doi.org/10.1016/j.jff.2016.12.033
url https://hdl.handle.net/11441/130762
https://doi.org/10.1016/j.jff.2016.12.033
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Functional Foods, 29, 238-247.
09-CVI-5007
ES-2012-056104
JCI-2012-13084
JAEDOC089
https://doi.org/10.1016/j.jff.2016.12.033
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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