Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.

Exosomes are cell-derived nanovesicles with a proven intercellular signaling role in inflammation processes and immune response. Due to their natural origin and liposome-like structure, these nanometer-scale vesicles have emerged as novel platforms for therapy and diagnosis. In this work, goat milk...

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Autores: Santos-Coquillat, Ana, González, María Isabel, Clemente-Moragón, Agustín, González-Arjona, Mario, Albaladejo-García, Virginia, Peinado, Héctor, Muñoz, Javier, Ximénez Embún, Pilar, Ibañez, Borja, Oliver, Eduardo, Desco, Manuel, Salinas, Beatriz
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/26064
Acceso en línea:https://hdl.handle.net/20.500.12105/26064
Access Level:acceso abierto
Palabra clave:exosomes
fluorescence imaging
goat milk
inflammation
macrophages
nanoparticles
peritonitis
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spelling Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.Santos-Coquillat, AnaGonzález, María IsabelClemente-Moragón, AgustínGonzález-Arjona, MarioAlbaladejo-García, VirginiaPeinado, HéctorMuñoz, JavierXiménez Embún, PilarIbañez, BorjaOliver, EduardoDesco, ManuelSalinas, Beatrizexosomesfluorescence imaginggoat milkinflammationmacrophagesnanoparticlesperitonitisExosomes are cell-derived nanovesicles with a proven intercellular signaling role in inflammation processes and immune response. Due to their natural origin and liposome-like structure, these nanometer-scale vesicles have emerged as novel platforms for therapy and diagnosis. In this work, goat milk exosomes are isolated and fully characterized in terms of their physicochemical properties, proteomics, and biochemical profile in healthy mice, and used to detect inflammatory processes by optical imaging. For the in vitro and in vivo experiments, the exosomes are covalently labeled with the commercial fluorophores sulfo-Cyanine 5 and BODIPY-FL to create nanoprobes. In vitro studies using confocal imaging, flow cytometry, and colorimetric assays confirm the internalization of the nanoprobes as well their lack of cytotoxicity in macrophage populations RAW 264.7. Optical imaging in the mouse peritoneal region confirms the in vivo ability of one of the nanoprobes to localize inflammatory processes. In vivo imaging shows exosome uptake in the inflamed peritoneal region, and flow-cytometric analysis of peritonitis exudates confirms the uptake by macrophage and neutrophil populations. These results support the promising use of goat milk exosomes as natural probes in the detection of inflammatory processes.WileyInstituto de Salud Carlos IIIUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)Comunidad de Madrid (España)Fundación BBVA20252025-01-1720222022-02-0120222022-02-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12105/26064reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengEuropean Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme PEJD-2018-POSTES Y2018 NMT-4949ES S2017 BMD-3867ES CD19 00136ES EPIC-XS 823839open accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/260642026-06-12T12:43:37Z
dc.title.none.fl_str_mv Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
title Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
spellingShingle Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
Santos-Coquillat, Ana
exosomes
fluorescence imaging
goat milk
inflammation
macrophages
nanoparticles
peritonitis
title_short Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
title_full Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
title_fullStr Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
title_full_unstemmed Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
title_sort Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging.
dc.creator.none.fl_str_mv Santos-Coquillat, Ana
González, María Isabel
Clemente-Moragón, Agustín
González-Arjona, Mario
Albaladejo-García, Virginia
Peinado, Héctor
Muñoz, Javier
Ximénez Embún, Pilar
Ibañez, Borja
Oliver, Eduardo
Desco, Manuel
Salinas, Beatriz
author Santos-Coquillat, Ana
author_facet Santos-Coquillat, Ana
González, María Isabel
Clemente-Moragón, Agustín
González-Arjona, Mario
Albaladejo-García, Virginia
Peinado, Héctor
Muñoz, Javier
Ximénez Embún, Pilar
Ibañez, Borja
Oliver, Eduardo
Desco, Manuel
Salinas, Beatriz
author_role author
author2 González, María Isabel
Clemente-Moragón, Agustín
González-Arjona, Mario
Albaladejo-García, Virginia
Peinado, Héctor
Muñoz, Javier
Ximénez Embún, Pilar
Ibañez, Borja
Oliver, Eduardo
Desco, Manuel
Salinas, Beatriz
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
Comunidad de Madrid (España)
Fundación BBVA
dc.subject.none.fl_str_mv exosomes
fluorescence imaging
goat milk
inflammation
macrophages
nanoparticles
peritonitis
topic exosomes
fluorescence imaging
goat milk
inflammation
macrophages
nanoparticles
peritonitis
description Exosomes are cell-derived nanovesicles with a proven intercellular signaling role in inflammation processes and immune response. Due to their natural origin and liposome-like structure, these nanometer-scale vesicles have emerged as novel platforms for therapy and diagnosis. In this work, goat milk exosomes are isolated and fully characterized in terms of their physicochemical properties, proteomics, and biochemical profile in healthy mice, and used to detect inflammatory processes by optical imaging. For the in vitro and in vivo experiments, the exosomes are covalently labeled with the commercial fluorophores sulfo-Cyanine 5 and BODIPY-FL to create nanoprobes. In vitro studies using confocal imaging, flow cytometry, and colorimetric assays confirm the internalization of the nanoprobes as well their lack of cytotoxicity in macrophage populations RAW 264.7. Optical imaging in the mouse peritoneal region confirms the in vivo ability of one of the nanoprobes to localize inflammatory processes. In vivo imaging shows exosome uptake in the inflamed peritoneal region, and flow-cytometric analysis of peritonitis exudates confirms the uptake by macrophage and neutrophil populations. These results support the promising use of goat milk exosomes as natural probes in the detection of inflammatory processes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-02-01
2022
2022-02-01
2025
2025-01-17
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/26064
url https://hdl.handle.net/20.500.12105/26064
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme PEJD-2018-POST
ES Y2018 NMT-4949
ES S2017 BMD-3867
ES CD19 00136
ES EPIC-XS 823839
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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