Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates

Bioconjugation; Polystyrene particles; Supramolecular structures

Detalles Bibliográficos
Autores: Casonato Melo, Cristiane, Fux, Alexandra, Himly, Martin, Schlahsa, Laura, Siewert, Christiane, Franco Puntes, Victor, G. Bastús, Neus
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/10416
Acceso en línea:https://hdl.handle.net/11351/10416
http://hdl.handle.net/11351/10416
Access Level:acceso abierto
Palabra clave:Immunofarmacologia
Toxines bacterianes
CHEMICALS AND DRUGS::Biological Factors::Toxins, Biological::Bacterial Toxins::Endotoxins
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoconjugates
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::toxinas biológicas::toxinas bacterianas::endotoxinas
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas sanguíneas::globulinas séricas::inmunoglobulinas::anticuerpos::inmunoconjugados
id ES_2d5cb9d66e3ee8be0db1ee320a5d7741
oai_identifier_str oai:recercat.cat:11351/10416
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
title Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
spellingShingle Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
Casonato Melo, Cristiane
Immunofarmacologia
Toxines bacterianes
CHEMICALS AND DRUGS::Biological Factors::Toxins, Biological::Bacterial Toxins::Endotoxins
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoconjugates
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::toxinas biológicas::toxinas bacterianas::endotoxinas
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas sanguíneas::globulinas séricas::inmunoglobulinas::anticuerpos::inmunoconjugados
title_short Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
title_full Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
title_fullStr Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
title_full_unstemmed Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
title_sort Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates
dc.creator.none.fl_str_mv Casonato Melo, Cristiane
Fux, Alexandra
Himly, Martin
Schlahsa, Laura
Siewert, Christiane
Franco Puntes, Victor
G. Bastús, Neus
author Casonato Melo, Cristiane
author_facet Casonato Melo, Cristiane
Fux, Alexandra
Himly, Martin
Schlahsa, Laura
Siewert, Christiane
Franco Puntes, Victor
G. Bastús, Neus
author_role author
author2 Fux, Alexandra
Himly, Martin
Schlahsa, Laura
Siewert, Christiane
Franco Puntes, Victor
G. Bastús, Neus
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Casonato Melo C, Fux AC] Chemical Biology Department, R&D Reagents, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany. Division of Allergy & Immunology, Department of Biosciences & Medical Biology, Paris Lodron University of Salzburg, Salzburg, Austria. [Himly M] Division of Allergy & Immunology, Department of Biosciences & Medical Biology, Paris Lodron University of Salzburg, Salzburg, Austria. [Bastús NG] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, Barcelona, Spain. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. [Schlahsa L, Siewert C] Chemical Biology Department, R&D Reagents, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany. [Puntes V] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, Barcelona, Spain. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Immunofarmacologia
Toxines bacterianes
CHEMICALS AND DRUGS::Biological Factors::Toxins, Biological::Bacterial Toxins::Endotoxins
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoconjugates
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::toxinas biológicas::toxinas bacterianas::endotoxinas
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas sanguíneas::globulinas séricas::inmunoglobulinas::anticuerpos::inmunoconjugados
topic Immunofarmacologia
Toxines bacterianes
CHEMICALS AND DRUGS::Biological Factors::Toxins, Biological::Bacterial Toxins::Endotoxins
CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoconjugates
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::toxinas biológicas::toxinas bacterianas::endotoxinas
COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas sanguíneas::globulinas séricas::inmunoglobulinas::anticuerpos::inmunoconjugados
description Bioconjugation; Polystyrene particles; Supramolecular structures
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/10416
http://hdl.handle.net/11351/10416
url https://hdl.handle.net/11351/10416
http://hdl.handle.net/11351/10416
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences;24(18)
https://doi.org/10.3390/ijms241813971
info:eu-repo/grantAgreement/EC/H2020/812661
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405310133731328
spelling Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle ConjugatesCasonato Melo, CristianeFux, AlexandraHimly, MartinSchlahsa, LauraSiewert, ChristianeFranco Puntes, VictorG. Bastús, NeusImmunofarmacologiaToxines bacterianesCHEMICALS AND DRUGS::Biological Factors::Toxins, Biological::Bacterial Toxins::EndotoxinsCHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::ImmunoconjugatesCOMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::toxinas biológicas::toxinas bacterianas::endotoxinasCOMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas sanguíneas::globulinas séricas::inmunoglobulinas::anticuerpos::inmunoconjugadosBioconjugation; Polystyrene particles; Supramolecular structuresBioconjugación; Partículas de poliestireno; Estructuras supramolecularesBioconjugació; Partícules de poliestirè; Estructures supramolecularsEndotoxins or lipopolysaccharides (LPS), found in the outer membrane of Gram-negative bacterial cell walls, can stimulate the human innate immune system, leading to life-threatening symptoms. Therefore, regulatory limits for endotoxin content apply to injectable pharmaceuticals, and excess LPS must be removed before commercialization. The majority of available endotoxin removal systems are based on the non-specific adsorption of LPS to charged and/or hydrophobic surfaces. Albeit effective to remove endotoxins, the lack of specificity can result in the unwanted loss of essential proteins from the pharmaceutical formulation. In this work, we developed microparticles conjugated to anti-Lipid A antibodies for selective endotoxin removal. Anti-Lipid A particles were characterized using flow cytometry and microscopy techniques. These particles exhibited a depletion capacity > 6 ×103 endotoxin units/mg particles from water, as determined with two independent methods (Limulus Amebocyte Lysate test and nanoparticle tracking analysis). Additionally, we compared these particles with a non-specific endotoxin removal system in a series of formulations of increasing complexity: bovine serum albumin in water < insulin in buffer < birch pollen extracts. We demonstrated that the specific anti-Lipid A particles show a higher protein recovery without compromising their endotoxin removal capacity. Consequently, we believe that the specificity layer integrated by the anti-Lipid A antibody could be advantageous to enhance product yield.The project leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 812661, H2020-MSCA-ITN-2018-812661 (ENDONANO).MDPIInstitut Català de la Salut[Casonato Melo C, Fux AC] Chemical Biology Department, R&D Reagents, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany. Division of Allergy & Immunology, Department of Biosciences & Medical Biology, Paris Lodron University of Salzburg, Salzburg, Austria. [Himly M] Division of Allergy & Immunology, Department of Biosciences & Medical Biology, Paris Lodron University of Salzburg, Salzburg, Austria. [Bastús NG] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, Barcelona, Spain. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. [Schlahsa L, Siewert C] Chemical Biology Department, R&D Reagents, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany. [Puntes V] Institut Català de Nanociència i Nanotecnologia (ICN2), Consejo Superior de Investigaciones Científicas (CSIC), The Barcelona Institute of Science and Technology (BIST), Campus UAB, Bellaterra, Barcelona, Spain. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, SpainVall d'Hebron Barcelona Hospital Campus202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/10416http://hdl.handle.net/11351/10416Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInternational Journal of Molecular Sciences;24(18)https://doi.org/10.3390/ijms241813971info:eu-repo/grantAgreement/EC/H2020/812661Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/104162026-05-29T05:05:01Z
score 15,811543

Similares en LA Referencia