Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells

A pH-triggered mesoporous silica nanoparticle (MSN)-based nano-vehicle for the dual delivery of doxorubicin (DOX)/camptothecin-PEG (CPT-PEG) has been prepared. To enhance its selectivity, the nanoparticles were decorated with glycyrrhetinic acid (GA) to target HepG2 cells. The highly insoluble CPT w...

Descripción completa

Detalles Bibliográficos
Autores: Sánchez-García, David, Borrós, Salvador, Fornaguera, Cristina, Martínez Edo, Gabriel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/1108
Acceso en línea:http://hdl.handle.net/20.500.14342/1108
https://doi.org/10.3390/pharmaceutics12111048
Access Level:acceso abierto
Palabra clave:Nanopartícules
Doxorubicina
Medicaments
Càncer
Mesoporous silica nanoparticles
Dual release
Doxorubicin
Camptothecin
Anticancer drugs
Combination therapy
Targeting systems
539
616
id ES_2d4575a116a1113a56bc771da367251f
oai_identifier_str oai:recercat.cat:20.500.14342/1108
network_acronym_str ES
network_name_str España
repository_id_str
spelling Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cellsSánchez-García, DavidBorrós, SalvadorFornaguera, CristinaMartínez Edo, GabrielNanopartículesDoxorubicinaMedicamentsCàncerMesoporous silica nanoparticlesDual releaseDoxorubicinCamptothecinAnticancer drugsCombination therapyTargeting systems539616A pH-triggered mesoporous silica nanoparticle (MSN)-based nano-vehicle for the dual delivery of doxorubicin (DOX)/camptothecin-PEG (CPT-PEG) has been prepared. To enhance its selectivity, the nanoparticles were decorated with glycyrrhetinic acid (GA) to target HepG2 cells. The highly insoluble CPT was derivatized with a reductive-cleavable PEG chain to improve its loading within the MSN. The preparation of these particles consisted of four steps. First, CPT-PEG was loaded within the pores of the MSN. Then, dihydrazide polyethylene glycol chains were introduced onto the surface of an aldehyde-functionalized MSN by means of a hydrazone bond. Afterwards, DOX was covalently attached to the other end of the dihydrazide polyethylene glycol chains. Finally, the resulting nanoparticles were decorated with GA by formation of an imine bond between the amino group of DOX and a benzaldehyde-GA derivative. The system was stable at physiological conditions and the release of both drugs was negligible. However, at acidic pH, a burst release of DOX and a gradual release of CPT-PEG takes place. GA-decorated drug delivery systems (DDS) selectively internalizes into HepG2. In vitro tests demonstrated that this system shows a great cytotoxicity towards HepG2 cells. Furthermore, glutathione cleavage of CPT prodrug assures the formation of free CPT leading to a synergistic effect in combination with DOX.MDPIUniversitat Ramon Llull. IQS2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion17 p.http://hdl.handle.net/20.500.14342/1108https://doi.org/10.3390/pharmaceutics12111048reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPharmaceutics. Vol.12, n.11 (2020), 1048info:eu-repo/grantAgreement/MCIU/PN I+D/RTI2018-094734-B-C22info:eu-repo/grantAgreement/SUR del DEC/SGR/2017-SGR-01559Attribution 4.0 International© L'autor/ahttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/11082026-05-29T05:05:01Z
dc.title.none.fl_str_mv Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
title Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
spellingShingle Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
Sánchez-García, David
Nanopartícules
Doxorubicina
Medicaments
Càncer
Mesoporous silica nanoparticles
Dual release
Doxorubicin
Camptothecin
Anticancer drugs
Combination therapy
Targeting systems
539
616
title_short Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
title_full Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
title_fullStr Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
title_full_unstemmed Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
title_sort Glycyrrhetinic acid-functionalized mesoporous silica nanoparticles for the co-delivery of DOX/CPT-PEG for targeting HepG2 cells
dc.creator.none.fl_str_mv Sánchez-García, David
Borrós, Salvador
Fornaguera, Cristina
Martínez Edo, Gabriel
author Sánchez-García, David
author_facet Sánchez-García, David
Borrós, Salvador
Fornaguera, Cristina
Martínez Edo, Gabriel
author_role author
author2 Borrós, Salvador
Fornaguera, Cristina
Martínez Edo, Gabriel
author2_role author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Nanopartícules
Doxorubicina
Medicaments
Càncer
Mesoporous silica nanoparticles
Dual release
Doxorubicin
Camptothecin
Anticancer drugs
Combination therapy
Targeting systems
539
616
topic Nanopartícules
Doxorubicina
Medicaments
Càncer
Mesoporous silica nanoparticles
Dual release
Doxorubicin
Camptothecin
Anticancer drugs
Combination therapy
Targeting systems
539
616
description A pH-triggered mesoporous silica nanoparticle (MSN)-based nano-vehicle for the dual delivery of doxorubicin (DOX)/camptothecin-PEG (CPT-PEG) has been prepared. To enhance its selectivity, the nanoparticles were decorated with glycyrrhetinic acid (GA) to target HepG2 cells. The highly insoluble CPT was derivatized with a reductive-cleavable PEG chain to improve its loading within the MSN. The preparation of these particles consisted of four steps. First, CPT-PEG was loaded within the pores of the MSN. Then, dihydrazide polyethylene glycol chains were introduced onto the surface of an aldehyde-functionalized MSN by means of a hydrazone bond. Afterwards, DOX was covalently attached to the other end of the dihydrazide polyethylene glycol chains. Finally, the resulting nanoparticles were decorated with GA by formation of an imine bond between the amino group of DOX and a benzaldehyde-GA derivative. The system was stable at physiological conditions and the release of both drugs was negligible. However, at acidic pH, a burst release of DOX and a gradual release of CPT-PEG takes place. GA-decorated drug delivery systems (DDS) selectively internalizes into HepG2. In vitro tests demonstrated that this system shows a great cytotoxicity towards HepG2 cells. Furthermore, glutathione cleavage of CPT prodrug assures the formation of free CPT leading to a synergistic effect in combination with DOX.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/1108
https://doi.org/10.3390/pharmaceutics12111048
url http://hdl.handle.net/20.500.14342/1108
https://doi.org/10.3390/pharmaceutics12111048
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Pharmaceutics. Vol.12, n.11 (2020), 1048
info:eu-repo/grantAgreement/MCIU/PN I+D/RTI2018-094734-B-C22
info:eu-repo/grantAgreement/SUR del DEC/SGR/2017-SGR-01559
dc.rights.none.fl_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17 p.
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405303779360768
score 15,81155

Similares en LA Referencia