Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1

14 Pags.- 7 Figs.- 1 Tabl. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (cc by 4.0)

Detalles Bibliográficos
Autores: Villar Rua, Irene, Rubio Luna, María Carmen, Calvo-Beguería, Laura, Pérez-Rontomé, Carmen, Larrainzar, Estíbaliz, Wilson, Michael T., Sandal, Niels, Mur, Luis A., Wang, Longlong, Reeder, Brandon, Duanmu, Deqiang, Uchiumi, Toshiki, Stougaard, Jens, Becana Ausejo, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/258651
Acceso en línea:http://hdl.handle.net/10261/258651
Access Level:acceso abierto
Palabra clave:Lotus japonicus
Medicago truncatula
Hemoglobins
mutants
nitric oxide
symbiosis
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spelling Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1Villar Rua, IreneRubio Luna, María CarmenCalvo-Beguería, LauraPérez-Rontomé, CarmenLarrainzar, EstíbalizWilson, Michael T.Sandal, NielsMur, Luis A.Wang, LonglongReeder, BrandonDuanmu, DeqiangUchiumi, ToshikiStougaard, JensBecana Ausejo, ManuelLotus japonicusMedicago truncatulaHemoglobinsHemoglobinsmutantsnitric oxidesymbiosis14 Pags.- 7 Figs.- 1 Tabl. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (cc by 4.0)Legumes express two major types of hemoglobins, namely symbiotic (leghemoglobins) and non-symbiotic (phytoglobins), with the latter being categorized into three classes according to phylogeny and biochemistry. Using knockout mutants, we show that all three phytoglobin classes are required for optimal vegetative and reproductive development of Lotus japonicus. The mutants of two class 1 phytoglobins showed different phenotypes: Ljglb1-1 plants were smaller and had relatively more pods, whereas Ljglb1-2 plants had no distinctive vegetative phenotype and produced relatively fewer pods. Non-nodulated plants lacking LjGlb2-1 showed delayed growth and alterations in the leaf metabolome linked to amino acid processing, fermentative and respiratory pathways, and hormonal balance. The leaves of mutant plants accumulated salicylic acid and contained relatively less methyl jasmonic acid, suggesting crosstalk between LjGlb2-1 and the signaling pathways of both hormones. Based on the expression of LjGlb2-1 in leaves, the alterations of flowering and fruiting of nodulated Ljglb2-1 plants, the developmental and biochemical phenotypes of the mutant fed on ammonium nitrate, and the heme coordination and reactivity of the protein toward nitric oxide, we conclude that LjGlb2-1 is not a leghemoglobin but an unusual class 2 phytoglobin. For comparison, we have also characterized a close relative of LjGlb2-1 in Medicago truncatula, MtLb3, and conclude that this is an atypical leghemoglobin.This work was supported by grants AGL2017-85775-R, PID2020-113985GB-I00, and RTI2018-094623-B-C22 from the Agencia Estatal de Investigación (AEI) and by Gobierno de Aragón (group A09_20R). IV was a ‘Formación de Personal Investigador’ fellow (BES-2015-073059) and EL is a ‘Ramón y Cajal’ fellow (RYC2018-023867-I), both funded by AEI.Peer reviewedOxford University PressSociety for Experimental BiologyAgencia Estatal de Investigación (España)Gobierno de AragónVillar Rua, Irene [0000-0003-4552-5655]Rubio Luna, María Carmen [0000-0002-9378-7775]Pérez-Rontomé, Carmen [0000-0002-9772-3170]Becana Ausejo, Manuel [0000-0002-1083-0804]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/258651reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020https://doi.org/10.1093/jxb/erab376Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2586512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
title Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
spellingShingle Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
Villar Rua, Irene
Lotus japonicus
Medicago truncatula
Hemoglobins
Hemoglobins
mutants
nitric oxide
symbiosis
title_short Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
title_full Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
title_fullStr Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
title_full_unstemmed Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
title_sort Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
dc.creator.none.fl_str_mv Villar Rua, Irene
Rubio Luna, María Carmen
Calvo-Beguería, Laura
Pérez-Rontomé, Carmen
Larrainzar, Estíbaliz
Wilson, Michael T.
Sandal, Niels
Mur, Luis A.
Wang, Longlong
Reeder, Brandon
Duanmu, Deqiang
Uchiumi, Toshiki
Stougaard, Jens
Becana Ausejo, Manuel
author Villar Rua, Irene
author_facet Villar Rua, Irene
Rubio Luna, María Carmen
Calvo-Beguería, Laura
Pérez-Rontomé, Carmen
Larrainzar, Estíbaliz
Wilson, Michael T.
Sandal, Niels
Mur, Luis A.
Wang, Longlong
Reeder, Brandon
Duanmu, Deqiang
Uchiumi, Toshiki
Stougaard, Jens
Becana Ausejo, Manuel
author_role author
author2 Rubio Luna, María Carmen
Calvo-Beguería, Laura
Pérez-Rontomé, Carmen
Larrainzar, Estíbaliz
Wilson, Michael T.
Sandal, Niels
Mur, Luis A.
Wang, Longlong
Reeder, Brandon
Duanmu, Deqiang
Uchiumi, Toshiki
Stougaard, Jens
Becana Ausejo, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Gobierno de Aragón
Villar Rua, Irene [0000-0003-4552-5655]
Rubio Luna, María Carmen [0000-0002-9378-7775]
Pérez-Rontomé, Carmen [0000-0002-9772-3170]
Becana Ausejo, Manuel [0000-0002-1083-0804]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lotus japonicus
Medicago truncatula
Hemoglobins
Hemoglobins
mutants
nitric oxide
symbiosis
topic Lotus japonicus
Medicago truncatula
Hemoglobins
Hemoglobins
mutants
nitric oxide
symbiosis
description 14 Pags.- 7 Figs.- 1 Tabl. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (cc by 4.0)
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/258651
url http://hdl.handle.net/10261/258651
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020MICIU/ICTI2017‐2020
https://doi.org/10.1093/jxb/erab376
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
Society for Experimental Biology
publisher.none.fl_str_mv Oxford University Press
Society for Experimental Biology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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