Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar

African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insuffici...

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Autores: Cadenas Fernández, Estefanía, Sánchez-Vizcaíno Rodríguez, José Manuel, Kosowska, Aleksandra, Rivera Arroyo, Belén, Mayoral Alegre, Francisco José, Rodríguez Bertos, Antonio Manuel, Yao, Jianxiu, Bray, Jocelyn, Lokhandwala, Shehnaz, Mwangi, Waithaka, Barasona García-Arévalo, José Ángel
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/8058
Acceso en línea:https://hdl.handle.net/20.500.14352/8058
Access Level:acceso abierto
Palabra clave:African swine fever
adenovirus
Armenia07/Arm07
Eurasian wild boar
immune response
subunit vaccine
protective efficacy
Animales salvajes y exóticos
Ganado porcino
Inmunología veterinaria
3105 Peces y Fauna Silvestre
3104.08 Porcinos
3109.03 Inmunología
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network_name_str España
repository_id_str
spelling Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild BoarCadenas Fernández, EstefaníaSánchez-Vizcaíno Rodríguez, José ManuelKosowska, AleksandraRivera Arroyo, BelénMayoral Alegre, Francisco JoséRodríguez Bertos, Antonio ManuelYao, JianxiuBray, JocelynLokhandwala, ShehnazMwangi, WaithakaBarasona García-Arévalo, José ÁngelAfrican swine feveradenovirusArmenia07/Arm07Eurasian wild boarimmune responsesubunit vaccineprotective efficacyAnimales salvajes y exóticosGanado porcinoInmunología veterinaria3105 Peces y Fauna Silvestre3104.08 Porcinos3109.03 InmunologíaAfrican swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine.MDPIUniversidad Complutense de Madrid20202020-02-2820202020-02-28journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/8058reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/80582026-06-02T12:44:21Z
dc.title.none.fl_str_mv Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
title Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
spellingShingle Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
Cadenas Fernández, Estefanía
African swine fever
adenovirus
Armenia07/Arm07
Eurasian wild boar
immune response
subunit vaccine
protective efficacy
Animales salvajes y exóticos
Ganado porcino
Inmunología veterinaria
3105 Peces y Fauna Silvestre
3104.08 Porcinos
3109.03 Inmunología
title_short Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
title_full Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
title_fullStr Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
title_full_unstemmed Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
title_sort Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
dc.creator.none.fl_str_mv Cadenas Fernández, Estefanía
Sánchez-Vizcaíno Rodríguez, José Manuel
Kosowska, Aleksandra
Rivera Arroyo, Belén
Mayoral Alegre, Francisco José
Rodríguez Bertos, Antonio Manuel
Yao, Jianxiu
Bray, Jocelyn
Lokhandwala, Shehnaz
Mwangi, Waithaka
Barasona García-Arévalo, José Ángel
author Cadenas Fernández, Estefanía
author_facet Cadenas Fernández, Estefanía
Sánchez-Vizcaíno Rodríguez, José Manuel
Kosowska, Aleksandra
Rivera Arroyo, Belén
Mayoral Alegre, Francisco José
Rodríguez Bertos, Antonio Manuel
Yao, Jianxiu
Bray, Jocelyn
Lokhandwala, Shehnaz
Mwangi, Waithaka
Barasona García-Arévalo, José Ángel
author_role author
author2 Sánchez-Vizcaíno Rodríguez, José Manuel
Kosowska, Aleksandra
Rivera Arroyo, Belén
Mayoral Alegre, Francisco José
Rodríguez Bertos, Antonio Manuel
Yao, Jianxiu
Bray, Jocelyn
Lokhandwala, Shehnaz
Mwangi, Waithaka
Barasona García-Arévalo, José Ángel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv African swine fever
adenovirus
Armenia07/Arm07
Eurasian wild boar
immune response
subunit vaccine
protective efficacy
Animales salvajes y exóticos
Ganado porcino
Inmunología veterinaria
3105 Peces y Fauna Silvestre
3104.08 Porcinos
3109.03 Inmunología
topic African swine fever
adenovirus
Armenia07/Arm07
Eurasian wild boar
immune response
subunit vaccine
protective efficacy
Animales salvajes y exóticos
Ganado porcino
Inmunología veterinaria
3105 Peces y Fauna Silvestre
3104.08 Porcinos
3109.03 Inmunología
description African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-02-28
2020
2020-02-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/8058
url https://hdl.handle.net/20.500.14352/8058
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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