Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1

14 páginas, 7 figuras

Detalles Bibliográficos
Autores: Rodriguez-Rodriguez, Jose-Antonio, Moyano, Yolanda, Játiva, Soraya, Queralt, Ethel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/272310
Acceso en línea:http://hdl.handle.net/10261/272310
Access Level:acceso abierto
Palabra clave:Cdc fourteen early anaphase release
Cdc14 phosphatase
Cdc5 polo kinase
Mitosis
Mitotic exit network
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spelling Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1Rodriguez-Rodriguez, Jose-AntonioMoyano, YolandaJátiva, SorayaQueralt, EthelCdc fourteen early anaphase releaseCdc14 phosphataseCdc5 polo kinaseMitosisMitotic exit network14 páginas, 7 figurasTo complete mitosis, Saccharomyces cerevisiae needs to activate the mitotic phosphatase Cdc14. Two pathways contribute to Cdc14 regulation: FEAR (Cdc14 early anaphase release) and MEN (mitotic exit network). Cdc5 polo-like kinase was found to be an important mitotic exit component. However, its specific role in mitotic exit regulation and its involvement in Cdc14 release remain unclear. Here, we provide insight into the mechanism by which Cdc5 contributes to the timely release of Cdc14. Our genetic and biochemical data indicate that Cdc5 acts in parallel with MEN during anaphase. This MEN-independent Cdc5 function requires active separase and activation by Cdk1-dependent phosphorylation. Cdk1 first phosphorylates Cdc5 to activate it in early anaphase, and then, in late anaphase, further phosphorylation of Cdc5 by Cdk1 is needed to promote its MEN-related functions.Our laboratory is supported by the Spanish Ministry of Science and Innovation (BFU2011-27568) and the Spanish Ministry of Economy and Competitiveness (BFU2013-43132-P). J.-.A.R.-R. is a recipient of an FPI studentshipPeer reviewedCell PressMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Queralt, Ethel [0000-0003-0045-0039]202220222016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/272310reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//BFU2011-27568info:eu-repo/grantAgreement/MINECO//BFU2013-43132-Phttps://dx.doi.org/10.1016/j.celrep.2016.04.079Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2723102026-05-22T06:33:51Z
dc.title.none.fl_str_mv Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
title Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
spellingShingle Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
Rodriguez-Rodriguez, Jose-Antonio
Cdc fourteen early anaphase release
Cdc14 phosphatase
Cdc5 polo kinase
Mitosis
Mitotic exit network
title_short Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
title_full Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
title_fullStr Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
title_full_unstemmed Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
title_sort Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1
dc.creator.none.fl_str_mv Rodriguez-Rodriguez, Jose-Antonio
Moyano, Yolanda
Játiva, Soraya
Queralt, Ethel
author Rodriguez-Rodriguez, Jose-Antonio
author_facet Rodriguez-Rodriguez, Jose-Antonio
Moyano, Yolanda
Játiva, Soraya
Queralt, Ethel
author_role author
author2 Moyano, Yolanda
Játiva, Soraya
Queralt, Ethel
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Queralt, Ethel [0000-0003-0045-0039]
dc.subject.none.fl_str_mv Cdc fourteen early anaphase release
Cdc14 phosphatase
Cdc5 polo kinase
Mitosis
Mitotic exit network
topic Cdc fourteen early anaphase release
Cdc14 phosphatase
Cdc5 polo kinase
Mitosis
Mitotic exit network
description 14 páginas, 7 figuras
publishDate 2016
dc.date.none.fl_str_mv 2016
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/272310
url http://hdl.handle.net/10261/272310
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//BFU2011-27568
info:eu-repo/grantAgreement/MINECO//BFU2013-43132-P
https://dx.doi.org/10.1016/j.celrep.2016.04.079
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405277093101568
score 15,81155

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