Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be i...

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Autores: Li, Yanli, Díaz, Ivan, Martín-Valls, Gerard, Beyersdorf, Niklas, Mateu, Enric
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/2284
Acceso en línea:http://hdl.handle.net/20.500.12327/2284
https://doi.org/10.3389/fimmu.2022.1020227
Access Level:acceso abierto
Palabra clave:619
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spelling Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infectionLi, YanliDíaz, IvanMartín-Valls, GerardBeyersdorf, NiklasMateu, Enric619Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be infected and cause transplacental infection. The immune effector mechanisms involved are largely unknown. Methods: The present study investigated the role of cytotoxic lymphocytes, including cytotoxic T cells (CTL), NKT, and NK cells, from blood in preventing PRRSV-1 transplacental infection in vaccinated primiparous sows (two doses vaccinated). Sows from a PRRSV-1 unstable farm were bled just before the last month of gestation (critical period for transplacental infection), then followed to determine whether sows delivered PRRSV-1-infected (n=8) or healthy (n=10) piglets. After that, functions of CTL, NKT, and NK cells in the two groups of sows were compared. Results: No difference was found through cell surface staining. But upon in vitro re-stimulation with the circulating field virus, sows that delivered healthy piglets displayed a higher frequency of virus-specific CD107a+ IFN-γ-producing T cells, which accumulated in the CD4+ compartment including CD4 single-positive (CD4 SP) and CD4/CD8α double-positive (CD4/CD8α DP) subsets. The same group of sows also harbored a higher proportion of CD107a+ TNF-α-producing T cells that predominantly accumulated in CD4/CD8α double-negative (CD4/CD8α DN) subset. Consistently, CD4 SP and CD4/CD8α DN T cells from sows delivering healthy piglets had a higher virus-specific proliferative response. Additionally, in sows that delivered PRRSV-1-infected piglets, a positive correlation of virus-specific IFN-γ response with average Ct values of umbilical cords of newborn piglets per litter was observed. Conclusion: Our data strongly suggest that CTL responses correlate with protection against PRRSV-1 transplacental infection, being executed by CD4 T cells (IFN-γ related) and/or CD4/CD8α DN T cells (TNF-α related).info:eu-repo/semantics/publishedVersionFrontiers MediaProducció AnimalSanitat Animal202320232023info:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/20.500.12327/2284https://doi.org/10.3389/fimmu.2022.1020227reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésFrontiers in ImmunologyAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/22842026-06-16T08:51:17Z
dc.title.none.fl_str_mv Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
title Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
spellingShingle Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
Li, Yanli
619
title_short Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
title_full Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
title_fullStr Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
title_full_unstemmed Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
title_sort Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection
dc.creator.none.fl_str_mv Li, Yanli
Díaz, Ivan
Martín-Valls, Gerard
Beyersdorf, Niklas
Mateu, Enric
author Li, Yanli
author_facet Li, Yanli
Díaz, Ivan
Martín-Valls, Gerard
Beyersdorf, Niklas
Mateu, Enric
author_role author
author2 Díaz, Ivan
Martín-Valls, Gerard
Beyersdorf, Niklas
Mateu, Enric
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows. Although modified-live virus (MLV) vaccines are available, only partial protection against heterologous strains is produced, thus vaccinated sows can be infected and cause transplacental infection. The immune effector mechanisms involved are largely unknown. Methods: The present study investigated the role of cytotoxic lymphocytes, including cytotoxic T cells (CTL), NKT, and NK cells, from blood in preventing PRRSV-1 transplacental infection in vaccinated primiparous sows (two doses vaccinated). Sows from a PRRSV-1 unstable farm were bled just before the last month of gestation (critical period for transplacental infection), then followed to determine whether sows delivered PRRSV-1-infected (n=8) or healthy (n=10) piglets. After that, functions of CTL, NKT, and NK cells in the two groups of sows were compared. Results: No difference was found through cell surface staining. But upon in vitro re-stimulation with the circulating field virus, sows that delivered healthy piglets displayed a higher frequency of virus-specific CD107a+ IFN-γ-producing T cells, which accumulated in the CD4+ compartment including CD4 single-positive (CD4 SP) and CD4/CD8α double-positive (CD4/CD8α DP) subsets. The same group of sows also harbored a higher proportion of CD107a+ TNF-α-producing T cells that predominantly accumulated in CD4/CD8α double-negative (CD4/CD8α DN) subset. Consistently, CD4 SP and CD4/CD8α DN T cells from sows delivering healthy piglets had a higher virus-specific proliferative response. Additionally, in sows that delivered PRRSV-1-infected piglets, a positive correlation of virus-specific IFN-γ response with average Ct values of umbilical cords of newborn piglets per litter was observed. Conclusion: Our data strongly suggest that CTL responses correlate with protection against PRRSV-1 transplacental infection, being executed by CD4 T cells (IFN-γ related) and/or CD4/CD8α DN T cells (TNF-α related).
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/2284
https://doi.org/10.3389/fimmu.2022.1020227
url http://hdl.handle.net/20.500.12327/2284
https://doi.org/10.3389/fimmu.2022.1020227
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Immunology
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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