Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus

Background: In the mouse uterus, pregnancy is accompanied by changes in tachykinin and tachykinin receptor gene expression and in the uterotonic effects of endogenous tachykinins. In this study we have investigated whether changes in tachykinin expression and responses are a result of changes in ova...

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Autores: Pinto Pérez, Francisco M., Pintado, C. Oscar, Pennefather, Jocelyn N., Patak, Eva, Candenas de Luján, María Luz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/72139
Acceso en línea:https://hdl.handle.net/11441/72139
https://doi.org/10.1186/1477-7827-7-77
Access Level:acceso abierto
Palabra clave:Ovarian steroids
Tachykinin
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spelling Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterusPinto Pérez, Francisco M.Pintado, C. OscarPennefather, Jocelyn N.Patak, EvaCandenas de Luján, María LuzOvarian steroidsTachykininBackground: In the mouse uterus, pregnancy is accompanied by changes in tachykinin and tachykinin receptor gene expression and in the uterotonic effects of endogenous tachykinins. In this study we have investigated whether changes in tachykinin expression and responses are a result of changes in ovarian steroid levels. Methods: We quantified the mRNAs of tachykinins and tachykinin receptors in uteri from ovariectomized mice and studied their regulation in response to estrogen and progesterone using real-time quantitative RT-PCR. Early (3 h) and late (24 h) responses to estrogen were evaluated and the participation of the estrogen receptors (ER), ERalpha and ERbeta, was analyzed by treating mice with propylpyrazole triol, a selective ERalpha agonist, or diarylpropionitrile, a selective agonist of ERbeta. Results: All genes encoding tachykinins (Tac1, Tac2 and Tac4) and tachykinin receptors (Tacr1, Tacr2 and Tacr3) were expressed in uteri from ovariectomized mice. Estrogen increased Tac1 and Tacr1 mRNA after 3 h and decreased Tac1 and Tac4 expression after 24 h. Tac2 and Tacr3 mRNA levels were decreased by estrogen at both 3 and 24 h. Most effects of estrogen were also observed in animals treated with propylpyrazole triol. Progesterone treatment increased the levels of Tac2. Conclusion: These results show that the expression of tachykinins and their receptors in the mouse uterus is tightly and differentially regulated by ovarian steroids. Estrogen effects are mainly mediated by ERalpha supporting an essential role for this estrogen receptor in the regulation of the tachykinergic system in the mouse uterus.Ministerio de Educación y Ciencia BFU2005-04495-C02-01/BFI, CTQ2007-61024/BQUJunta de Andalucía FQM261BioMed CentralMinisterio de Educación y Ciencia (MEC). EspañaJunta de Andalucía2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/72139https://doi.org/10.1186/1477-7827-7-77reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésReproductive Biology and Endocrinology, 7 (77)BFU2005-04495-C02-01/BFICTQ2007-61024/BQUFQM261http://dx.doi.org/10.1186/1477-7827-7-77info:eu-repo/semantics/openAccessoai:idus.us.es:11441/721392026-06-17T12:51:07Z
dc.title.none.fl_str_mv Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
title Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
spellingShingle Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
Pinto Pérez, Francisco M.
Ovarian steroids
Tachykinin
title_short Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
title_full Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
title_fullStr Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
title_full_unstemmed Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
title_sort Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
dc.creator.none.fl_str_mv Pinto Pérez, Francisco M.
Pintado, C. Oscar
Pennefather, Jocelyn N.
Patak, Eva
Candenas de Luján, María Luz
author Pinto Pérez, Francisco M.
author_facet Pinto Pérez, Francisco M.
Pintado, C. Oscar
Pennefather, Jocelyn N.
Patak, Eva
Candenas de Luján, María Luz
author_role author
author2 Pintado, C. Oscar
Pennefather, Jocelyn N.
Patak, Eva
Candenas de Luján, María Luz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (MEC). España
Junta de Andalucía
dc.subject.none.fl_str_mv Ovarian steroids
Tachykinin
topic Ovarian steroids
Tachykinin
description Background: In the mouse uterus, pregnancy is accompanied by changes in tachykinin and tachykinin receptor gene expression and in the uterotonic effects of endogenous tachykinins. In this study we have investigated whether changes in tachykinin expression and responses are a result of changes in ovarian steroid levels. Methods: We quantified the mRNAs of tachykinins and tachykinin receptors in uteri from ovariectomized mice and studied their regulation in response to estrogen and progesterone using real-time quantitative RT-PCR. Early (3 h) and late (24 h) responses to estrogen were evaluated and the participation of the estrogen receptors (ER), ERalpha and ERbeta, was analyzed by treating mice with propylpyrazole triol, a selective ERalpha agonist, or diarylpropionitrile, a selective agonist of ERbeta. Results: All genes encoding tachykinins (Tac1, Tac2 and Tac4) and tachykinin receptors (Tacr1, Tacr2 and Tacr3) were expressed in uteri from ovariectomized mice. Estrogen increased Tac1 and Tacr1 mRNA after 3 h and decreased Tac1 and Tac4 expression after 24 h. Tac2 and Tacr3 mRNA levels were decreased by estrogen at both 3 and 24 h. Most effects of estrogen were also observed in animals treated with propylpyrazole triol. Progesterone treatment increased the levels of Tac2. Conclusion: These results show that the expression of tachykinins and their receptors in the mouse uterus is tightly and differentially regulated by ovarian steroids. Estrogen effects are mainly mediated by ERalpha supporting an essential role for this estrogen receptor in the regulation of the tachykinergic system in the mouse uterus.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/72139
https://doi.org/10.1186/1477-7827-7-77
url https://hdl.handle.net/11441/72139
https://doi.org/10.1186/1477-7827-7-77
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproductive Biology and Endocrinology, 7 (77)
BFU2005-04495-C02-01/BFI
CTQ2007-61024/BQU
FQM261
http://dx.doi.org/10.1186/1477-7827-7-77
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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