Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility

Extracellular vesicles (EVs) are key mediators of cell-to-cell communication. Their content reflects the state of diseased cells representing a window into disease progression. Collagen-VI Related Muscular Dystrophy (COL6-RD) is a multi-systemic disease involving different cell types. The role of EV...

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Autores: Badosa, Carmen, Roldán Molina, Mónica, Fernández-Irigoyen, Joaquín, Santamaria, Enrique, Jiménez-Mallebrera, Cecilia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/217277
Acceso en línea:https://hdl.handle.net/2445/217277
Access Level:acceso abierto
Palabra clave:Proteòmica
Fibroblasts
Interacció cel·lular
Col·lagen
Distròfia muscular
Proteomics
Cell interaction
Collagen
Muscular dystrophy
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spelling Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motilityBadosa, CarmenRoldán Molina, MónicaFernández-Irigoyen, JoaquínSantamaria, EnriqueJiménez-Mallebrera, CeciliaProteòmicaFibroblastsInteracció cel·lularCol·lagenDistròfia muscularProteomicsFibroblastsCell interactionCollagenMuscular dystrophyExtracellular vesicles (EVs) are key mediators of cell-to-cell communication. Their content reflects the state of diseased cells representing a window into disease progression. Collagen-VI Related Muscular Dystrophy (COL6-RD) is a multi-systemic disease involving different cell types. The role of EVs in this disease has not been explored. We compared by quantitative proteomics the protein cargo of EVs released from fibroblasts from patients with COL6-RD and controls. Isolated EVs contained a significant proportion of the most frequently reported proteins in EVs according to Exocarta and Vesiclepedia. We identified 67 differentially abundant proteins associated with vesicle transport and exocytosis, actin remodelling and the cytoskeleton, hemostasis and oxidative stress. Treatment of control fibroblasts with EVs from either patient or healthy fibroblasts altered significantly the motility of cells on a cell migration assay highlighting the functional relevance of EVs. In parallel, we analysed the secretome from the same cells and found a distinctly different set of 48 differentially abundant proteins related to extracellular matrix organisation and remodelling, growth factor response, RNA metabolism and the proteasome. The EVs and secretome sets of proteins only shared two identifiers indicating that the sorting of proteins towards EVs or the secretory pathway is tightly regulated for different functions.Nature Publishing Group2025202520232025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/217277Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-023-41632-1Scientific Reports, 2023, vol. 13, num.1, p. 1-13https://doi.org/10.1038/s41598-023-41632-1cc-by (c) Badosa Carmen et al., 2023http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2172772026-05-29T05:05:01Z
dc.title.none.fl_str_mv Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
title Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
spellingShingle Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
Badosa, Carmen
Proteòmica
Fibroblasts
Interacció cel·lular
Col·lagen
Distròfia muscular
Proteomics
Fibroblasts
Cell interaction
Collagen
Muscular dystrophy
title_short Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
title_full Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
title_fullStr Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
title_full_unstemmed Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
title_sort Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility
dc.creator.none.fl_str_mv Badosa, Carmen
Roldán Molina, Mónica
Fernández-Irigoyen, Joaquín
Santamaria, Enrique
Jiménez-Mallebrera, Cecilia
author Badosa, Carmen
author_facet Badosa, Carmen
Roldán Molina, Mónica
Fernández-Irigoyen, Joaquín
Santamaria, Enrique
Jiménez-Mallebrera, Cecilia
author_role author
author2 Roldán Molina, Mónica
Fernández-Irigoyen, Joaquín
Santamaria, Enrique
Jiménez-Mallebrera, Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Proteòmica
Fibroblasts
Interacció cel·lular
Col·lagen
Distròfia muscular
Proteomics
Fibroblasts
Cell interaction
Collagen
Muscular dystrophy
topic Proteòmica
Fibroblasts
Interacció cel·lular
Col·lagen
Distròfia muscular
Proteomics
Fibroblasts
Cell interaction
Collagen
Muscular dystrophy
description Extracellular vesicles (EVs) are key mediators of cell-to-cell communication. Their content reflects the state of diseased cells representing a window into disease progression. Collagen-VI Related Muscular Dystrophy (COL6-RD) is a multi-systemic disease involving different cell types. The role of EVs in this disease has not been explored. We compared by quantitative proteomics the protein cargo of EVs released from fibroblasts from patients with COL6-RD and controls. Isolated EVs contained a significant proportion of the most frequently reported proteins in EVs according to Exocarta and Vesiclepedia. We identified 67 differentially abundant proteins associated with vesicle transport and exocytosis, actin remodelling and the cytoskeleton, hemostasis and oxidative stress. Treatment of control fibroblasts with EVs from either patient or healthy fibroblasts altered significantly the motility of cells on a cell migration assay highlighting the functional relevance of EVs. In parallel, we analysed the secretome from the same cells and found a distinctly different set of 48 differentially abundant proteins related to extracellular matrix organisation and remodelling, growth factor response, RNA metabolism and the proteasome. The EVs and secretome sets of proteins only shared two identifiers indicating that the sorting of proteins towards EVs or the secretory pathway is tightly regulated for different functions.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/217277
url https://hdl.handle.net/2445/217277
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-023-41632-1
Scientific Reports, 2023, vol. 13, num.1, p. 1-13
https://doi.org/10.1038/s41598-023-41632-1
dc.rights.none.fl_str_mv cc-by (c) Badosa Carmen et al., 2023
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Badosa Carmen et al., 2023
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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