Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication

Historically, gilthead seabream (Sparus aurata) has been considered a fish species resistant to nervous necrosis virus (NNV) disease. Nevertheless, mortality in seabream hatcheries, associated with typical clinical signs of the viral encephalopathy and retinopathy (VER) disease has been confirmed to...

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Autores: García-Álvarez, Miguel Ángel, Arizcun, Marta, Chaves-Pozo, Elena, Cuesta, Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313411
Acceso en línea:http://hdl.handle.net/10261/313411
Access Level:acceso abierto
Palabra clave:Centro Oceanográfico de Murcia
nodavirus
Acuicultura
reassortants
virus
Gilthead seabream
larvae
Immunity
fish
Viruses
Larvae
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spelling Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and ReplicationGarcía-Álvarez, Miguel ÁngelArizcun, MartaChaves-Pozo, ElenaCuesta, AlbertoCentro Oceanográfico de MurcianodavirusAcuiculturareassortantsvirusGilthead seabreamlarvaeImmunityfishVirusesImmunityLarvaeHistorically, gilthead seabream (Sparus aurata) has been considered a fish species resistant to nervous necrosis virus (NNV) disease. Nevertheless, mortality in seabream hatcheries, associated with typical clinical signs of the viral encephalopathy and retinopathy (VER) disease has been confirmed to be caused by RGNNV/SJNNV reassortants. Because of this, seabream larvae at 37 and 86 days post-hatching (dph) were infected by immersion with RGNNV/SJNNV and SJNNV/RGNNV reassortants under laboratory conditions, and mortality, viral replication and immunity were evaluated. Our results show that gilthead seabream larvae, mainly those at 37 dph, are susceptible to infection with both NNV reassortant genotypes, with the highest impact from the RGNNV/SJNNV reassortant. In addition, viral replication occurs at both ages (37 and 86 dph) but the recovery of infective particles was only confirmed in 37 dph larvae,; this value was also highest with the RGNNV/SJNNV reassortant. Larvae immunity, including the expression of antiviral, inflammatory and cell-mediated cytotoxicity genes, was affected by NNV infection. Levels of the natural killer lysin (Nkl) peptide were increased in SJNNV/RGNNV-infected larvae of 37 dph, though hepcidin was not. Our results demonstrate that the seabream larvae are susceptible to both NNV reassortants, though mainly to RGNNV/SJNNV, in an age-dependent manner.ALGAFISHEvaluación de los efectos de las microalgas, incluidas en dietas de dorada, sobre el crecimiento, supervivencia y resistencia a infecciones.SI202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/313411reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#Ministerio de Economía y Competitividad and FEDER /grant RTI2018-096625-B-C33Centro Oceanográfico de Murciahttps://doi.org/10.3390/ijms23095092info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3134112026-05-22T06:33:51Z
dc.title.none.fl_str_mv Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
title Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
spellingShingle Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
García-Álvarez, Miguel Ángel
Centro Oceanográfico de Murcia
nodavirus
Acuicultura
reassortants
virus
Gilthead seabream
larvae
Immunity
fish
Viruses
Immunity
Larvae
title_short Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
title_full Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
title_fullStr Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
title_full_unstemmed Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
title_sort Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication
dc.creator.none.fl_str_mv García-Álvarez, Miguel Ángel
Arizcun, Marta
Chaves-Pozo, Elena
Cuesta, Alberto
author García-Álvarez, Miguel Ángel
author_facet García-Álvarez, Miguel Ángel
Arizcun, Marta
Chaves-Pozo, Elena
Cuesta, Alberto
author_role author
author2 Arizcun, Marta
Chaves-Pozo, Elena
Cuesta, Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Centro Oceanográfico de Murcia
nodavirus
Acuicultura
reassortants
virus
Gilthead seabream
larvae
Immunity
fish
Viruses
Immunity
Larvae
topic Centro Oceanográfico de Murcia
nodavirus
Acuicultura
reassortants
virus
Gilthead seabream
larvae
Immunity
fish
Viruses
Immunity
Larvae
description Historically, gilthead seabream (Sparus aurata) has been considered a fish species resistant to nervous necrosis virus (NNV) disease. Nevertheless, mortality in seabream hatcheries, associated with typical clinical signs of the viral encephalopathy and retinopathy (VER) disease has been confirmed to be caused by RGNNV/SJNNV reassortants. Because of this, seabream larvae at 37 and 86 days post-hatching (dph) were infected by immersion with RGNNV/SJNNV and SJNNV/RGNNV reassortants under laboratory conditions, and mortality, viral replication and immunity were evaluated. Our results show that gilthead seabream larvae, mainly those at 37 dph, are susceptible to infection with both NNV reassortant genotypes, with the highest impact from the RGNNV/SJNNV reassortant. In addition, viral replication occurs at both ages (37 and 86 dph) but the recovery of infective particles was only confirmed in 37 dph larvae,; this value was also highest with the RGNNV/SJNNV reassortant. Larvae immunity, including the expression of antiviral, inflammatory and cell-mediated cytotoxicity genes, was affected by NNV infection. Levels of the natural killer lysin (Nkl) peptide were increased in SJNNV/RGNNV-infected larvae of 37 dph, though hepcidin was not. Our results demonstrate that the seabream larvae are susceptible to both NNV reassortants, though mainly to RGNNV/SJNNV, in an age-dependent manner.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313411
url http://hdl.handle.net/10261/313411
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
Ministerio de Economía y Competitividad and FEDER /grant RTI2018-096625-B-C33
Centro Oceanográfico de Murcia
https://doi.org/10.3390/ijms23095092
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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