Studying ion transport dynamics in electrochemical measurements of lateral flow assays

Le Brun, Grégoire; Calucho, Enric|||0000-0002-6024-765X; Hauwaert, Margo; Moumneh, Ramy; Maroli, Gabriel|||0000-0001-8921-4205; Yunus, Sami; Rosati, Giulio|||0000-0002-0227-4561; Álvarez Diduk, Ruslan|||0000-0002-9876-1574; Piper, Andrew|||0000-0002-1208-8009; Merkoçi, Arben|||0000-0003-2486-8085; Raskin, Jean-Pierre

Studying ion transport dynamics in electrochemical measurements of lateral flow assays

Lateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a sign...

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Autores:	Le Brun, Grégoire, Calucho, Enric\|\|\|0000-0002-6024-765X, Hauwaert, Margo, Moumneh, Ramy, Maroli, Gabriel\|\|\|0000-0001-8921-4205, Yunus, Sami, Rosati, Giulio\|\|\|0000-0002-0227-4561, Álvarez Diduk, Ruslan\|\|\|0000-0002-9876-1574, Piper, Andrew\|\|\|0000-0002-1208-8009, Merkoçi, Arben\|\|\|0000-0003-2486-8085, Raskin, Jean-Pierre
Tipo de recurso:	artículo
Fecha de publicación:	2024
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:302511
Acceso en línea:	https://ddd.uab.cat/record/302511 https://dx.doi.org/urn:doi:10.1016/j.jelechem.2024.118399
Access Level:	acceso abierto
Palabra clave:	Lateral flow assays Electrochemistry Electrochemical impedance spectroscopy Microfluidics Ion transport Point-of-care

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spelling	Studying ion transport dynamics in electrochemical measurements of lateral flow assaysLe Brun, GrégoireCalucho, Enric\|\|\|0000-0002-6024-765XHauwaert, MargoMoumneh, RamyMaroli, Gabriel\|\|\|0000-0001-8921-4205Yunus, SamiRosati, Giulio\|\|\|0000-0002-0227-4561Álvarez Diduk, Ruslan\|\|\|0000-0002-9876-1574Piper, Andrew\|\|\|0000-0002-1208-8009Merkoçi, Arben\|\|\|0000-0003-2486-8085Raskin, Jean-PierreLateral flow assaysElectrochemistryElectrochemical impedance spectroscopyMicrofluidicsIon transportPoint-of-careLateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a significant challenge in the development of new eLFAs. In this work, impedimetric measurements were performed to probe the impact of experimental factors such as electrolyte conductivity/ionic strength on the eLFA outcomes. The time-dependent evolution of non-Faradaic impedance measurements coupled with flow velocity computations were employed to investigate ion transport across different LFA configurations and buffer compositions. The analysis of the Péclet number, a characteristic dimensionless parameter of flow dynamics within the LFA, revealed the coexistence of diffusive and convective ionic transport regimes over the LFA operational time. In addition, our findings underscore the critical role of absorbent pad dead volume adjustments in governing the capacity to maintain sample flow within the membrane over extended durations. These electro-fluidics phenomena are essential considerations for conducting electrochemical measurements within LFAs. Overall, this study offers insights into key design parameters for the integration of electrochemistry into LFAs. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/302511https://dx.doi.org/urn:doi:10.1016/j.jelechem.2024.118399reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PRE2018-084856open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3025112026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
spellingShingle	Studying ion transport dynamics in electrochemical measurements of lateral flow assays Le Brun, Grégoire Lateral flow assays Electrochemistry Electrochemical impedance spectroscopy Microfluidics Ion transport Point-of-care
title_short	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_full	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_fullStr	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_full_unstemmed	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_sort	Studying ion transport dynamics in electrochemical measurements of lateral flow assays
dc.creator.none.fl_str_mv	Le Brun, Grégoire Calucho, Enric\|\|\|0000-0002-6024-765X Hauwaert, Margo Moumneh, Ramy Maroli, Gabriel\|\|\|0000-0001-8921-4205 Yunus, Sami Rosati, Giulio\|\|\|0000-0002-0227-4561 Álvarez Diduk, Ruslan\|\|\|0000-0002-9876-1574 Piper, Andrew\|\|\|0000-0002-1208-8009 Merkoçi, Arben\|\|\|0000-0003-2486-8085 Raskin, Jean-Pierre
author	Le Brun, Grégoire
author_facet	Le Brun, Grégoire Calucho, Enric\|\|\|0000-0002-6024-765X Hauwaert, Margo Moumneh, Ramy Maroli, Gabriel\|\|\|0000-0001-8921-4205 Yunus, Sami Rosati, Giulio\|\|\|0000-0002-0227-4561 Álvarez Diduk, Ruslan\|\|\|0000-0002-9876-1574 Piper, Andrew\|\|\|0000-0002-1208-8009 Merkoçi, Arben\|\|\|0000-0003-2486-8085 Raskin, Jean-Pierre
author_role	author
author2	Calucho, Enric\|\|\|0000-0002-6024-765X Hauwaert, Margo Moumneh, Ramy Maroli, Gabriel\|\|\|0000-0001-8921-4205 Yunus, Sami Rosati, Giulio\|\|\|0000-0002-0227-4561 Álvarez Diduk, Ruslan\|\|\|0000-0002-9876-1574 Piper, Andrew\|\|\|0000-0002-1208-8009 Merkoçi, Arben\|\|\|0000-0003-2486-8085 Raskin, Jean-Pierre
author2_role	author author author author author author author author author author
dc.subject.none.fl_str_mv	Lateral flow assays Electrochemistry Electrochemical impedance spectroscopy Microfluidics Ion transport Point-of-care
topic	Lateral flow assays Electrochemistry Electrochemical impedance spectroscopy Microfluidics Ion transport Point-of-care
description	Lateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a significant challenge in the development of new eLFAs. In this work, impedimetric measurements were performed to probe the impact of experimental factors such as electrolyte conductivity/ionic strength on the eLFA outcomes. The time-dependent evolution of non-Faradaic impedance measurements coupled with flow velocity computations were employed to investigate ion transport across different LFA configurations and buffer compositions. The analysis of the Péclet number, a characteristic dimensionless parameter of flow dynamics within the LFA, revealed the coexistence of diffusive and convective ionic transport regimes over the LFA operational time. In addition, our findings underscore the critical role of absorbent pad dead volume adjustments in governing the capacity to maintain sample flow within the membrane over extended durations. These electro-fluidics phenomena are essential considerations for conducting electrochemical measurements within LFAs. Overall, this study offers insights into key design parameters for the integration of electrochemistry into LFAs.
publishDate	2024
dc.date.none.fl_str_mv	2 2024-01-01 2024 2024-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/302511 https://dx.doi.org/urn:doi:10.1016/j.jelechem.2024.118399
url	https://ddd.uab.cat/record/302511 https://dx.doi.org/urn:doi:10.1016/j.jelechem.2024.118399
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PRE2018-084856
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Studying ion transport dynamics in electrochemical measurements of lateral flow assays

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