Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm

Aneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The role of peripheral blood mononuclear cells (PBMCs) in this response is a curr...

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Autores: Revilla Gonzalez, Gonzalo, Varela Pérez, Lourdes María, Ruiz de Azua-López, Zaida, Amaya-Villar, Rosario, Rosa Pezzotti, Maria, Castro, María José, Ureña López, Juan, González-Montelongo, María del Carmen, Castellano Orozco, Antonio Gonzalo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/147518
Acceso en línea:https://hdl.handle.net/11441/147518
https://doi.org/10.1007/s12975-023-01136-6
Access Level:acceso abierto
Palabra clave:Aneurysmal subarachnoid hemorrhage (aSAH)
Peripheral blood mononuclear cells (PBMCs)
Vasospasm (VSP)
Adhesion to the endothelium
Adhesion molecules
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spelling Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasmRevilla Gonzalez, GonzaloVarela Pérez, Lourdes MaríaRuiz de Azua-López, ZaidaAmaya-Villar, RosarioRosa Pezzotti, MariaCastro, María JoséUreña López, JuanGonzález-Montelongo, María del CarmenCastellano Orozco, Antonio GonzaloAneurysmal subarachnoid hemorrhage (aSAH)Peripheral blood mononuclear cells (PBMCs)Vasospasm (VSP)Adhesion to the endotheliumAdhesion moleculesAneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The role of peripheral blood mononuclear cells (PBMCs) in this response is a current subject of research. We have analysed the changes in PBMCs of patients with aSAH and their interaction with the endothelium, focusing on their adhesion and the expression of adhesion molecules. Using an in vitro adhesion assay, we observed that the adhesion of PBMCs of patients with aSAH is increased. Flow cytometry analysis shows that monocytes increased signifcantly in patients, especially in those who developed vasospasm (VSP). In aSAH patients, the expression of CD162, CD49d, CD62L and CD11a in T lymphocytes and of CD62L in monocytes increased. However, the expression of CD162, CD43, and CD11a decreased in monocytes. Furthermore, monocytes from patients who developed arteriographic VSP had lower expression of CD62L. In conclusion, our results confrm that after aSAH, monocyte count and adhesion of PBMCs increase, especially in patients with VSP, and that the expression of several adhesion molecules is altered. These observations can help predict VSP and to improve the treatment of this pathology.SpringerFisiología Médica y BiofísicaUniversidad de Sevilla/ CBUA2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/147518https://doi.org/10.1007/s12975-023-01136-6reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésTranslational Stroke ResearchSAF2017- 89474-Rhttps://link.springer.com/article/10.1007/s12975-023-01136-6info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1475182026-06-17T12:51:07Z
dc.title.none.fl_str_mv Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
title Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
spellingShingle Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
Revilla Gonzalez, Gonzalo
Aneurysmal subarachnoid hemorrhage (aSAH)
Peripheral blood mononuclear cells (PBMCs)
Vasospasm (VSP)
Adhesion to the endothelium
Adhesion molecules
title_short Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
title_full Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
title_fullStr Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
title_full_unstemmed Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
title_sort Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
dc.creator.none.fl_str_mv Revilla Gonzalez, Gonzalo
Varela Pérez, Lourdes María
Ruiz de Azua-López, Zaida
Amaya-Villar, Rosario
Rosa Pezzotti, Maria
Castro, María José
Ureña López, Juan
González-Montelongo, María del Carmen
Castellano Orozco, Antonio Gonzalo
author Revilla Gonzalez, Gonzalo
author_facet Revilla Gonzalez, Gonzalo
Varela Pérez, Lourdes María
Ruiz de Azua-López, Zaida
Amaya-Villar, Rosario
Rosa Pezzotti, Maria
Castro, María José
Ureña López, Juan
González-Montelongo, María del Carmen
Castellano Orozco, Antonio Gonzalo
author_role author
author2 Varela Pérez, Lourdes María
Ruiz de Azua-López, Zaida
Amaya-Villar, Rosario
Rosa Pezzotti, Maria
Castro, María José
Ureña López, Juan
González-Montelongo, María del Carmen
Castellano Orozco, Antonio Gonzalo
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fisiología Médica y Biofísica
Universidad de Sevilla/ CBUA
dc.subject.none.fl_str_mv Aneurysmal subarachnoid hemorrhage (aSAH)
Peripheral blood mononuclear cells (PBMCs)
Vasospasm (VSP)
Adhesion to the endothelium
Adhesion molecules
topic Aneurysmal subarachnoid hemorrhage (aSAH)
Peripheral blood mononuclear cells (PBMCs)
Vasospasm (VSP)
Adhesion to the endothelium
Adhesion molecules
description Aneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The role of peripheral blood mononuclear cells (PBMCs) in this response is a current subject of research. We have analysed the changes in PBMCs of patients with aSAH and their interaction with the endothelium, focusing on their adhesion and the expression of adhesion molecules. Using an in vitro adhesion assay, we observed that the adhesion of PBMCs of patients with aSAH is increased. Flow cytometry analysis shows that monocytes increased signifcantly in patients, especially in those who developed vasospasm (VSP). In aSAH patients, the expression of CD162, CD49d, CD62L and CD11a in T lymphocytes and of CD62L in monocytes increased. However, the expression of CD162, CD43, and CD11a decreased in monocytes. Furthermore, monocytes from patients who developed arteriographic VSP had lower expression of CD62L. In conclusion, our results confrm that after aSAH, monocyte count and adhesion of PBMCs increase, especially in patients with VSP, and that the expression of several adhesion molecules is altered. These observations can help predict VSP and to improve the treatment of this pathology.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/147518
https://doi.org/10.1007/s12975-023-01136-6
url https://hdl.handle.net/11441/147518
https://doi.org/10.1007/s12975-023-01136-6
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Translational Stroke Research
SAF2017- 89474-R
https://link.springer.com/article/10.1007/s12975-023-01136-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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