Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases

17 Pág.

Detalles Bibliográficos
Autores: Cerna-Vargas, Jean Paul, Krell, Tino
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/359623
Acceso en línea:http://hdl.handle.net/10261/359623
https://api.elsevier.com/content/abstract/scopus_id/85193922034
Access Level:acceso abierto
Palabra clave:Pseudomonas aeruginosa
Molecular recognition
Sensor histidine kinase
Signal transduction
Solute binding proteins
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spelling Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinasesCerna-Vargas, Jean PaulKrell, TinoPseudomonas aeruginosaMolecular recognitionSensor histidine kinaseSignal transductionSolute binding proteins17 Pág.The standard method of receptor activation involves the binding of signals or signal-loaded solute binding proteins (SBPs) to sensor domains. Many sensor histidine kinases (SHKs), which are activated by SBP binding, are encoded adjacent to their corresponding sbp gene. We examined three SBPs of Pseudomonas aeruginosa PAO1, encoded near the genes for the AgtS (PA0600) and AruS (PA4982) SHKs, to determine how common this arrangement is. Ligand screening and microcalorimetric studies revealed that the SBPs PA0602 and PA4985 preferentially bind to GABA (KD = 2.3 and 0.58 μM, respectively), followed by 5-aminovalerate (KD = 30 and 1.6 μM, respectively) and ethanoldiamine (KD = 2.3 and 0.58 μM, respectively). In contrast, AgtB (PA0604) exclusively recognizes 5-aminovaleric acid (KD = 2.9 μM). However, microcalorimetric titrations did not show any binding between the AgtS sensor domain and AgtB or PA0602, regardless of the presence of ligands. Similarly, bacterial two-hybrid assays did not demonstrate an interaction between PA4985 and the AruS sensor domain. Therefore, sbp and shk genes located nearby are not always functionally linked. We previously identified PA0222 as a GABA-specific SBP. The presence of three SBPs for GABA may be linked to GABA's role as a trigger for P. aeruginosa virulence.This work was supported by the Spanish Ministry for Science and Innovation/Agencia Estatal de Investigación 10.13039/501100011033 (grant PID2020-112612GB-I00 to T. K.) and the Junta de Andalucía (grant P18-FR-1621 to T. K.). J. P. C. V. was supported by the grant Unión Europea-NextGenerationEU RD 289/2021 UPM-Recualifica Margarita Salas.Peer reviewedJohn Wiley & SonsMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Cerna-Vargas, Jean Paul [0000-0001-5489-0385]Krell, Tino [0000-0002-9040-3166]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/359623https://api.elsevier.com/content/abstract/scopus_id/85193922034reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112612GB-I00Centro de Biotecnología y Genómica de Plantas (CBGP)ttps://doi.org/10.1002/mbo3.1415Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3596232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
title Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
spellingShingle Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
Cerna-Vargas, Jean Paul
Pseudomonas aeruginosa
Molecular recognition
Sensor histidine kinase
Signal transduction
Solute binding proteins
title_short Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
title_full Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
title_fullStr Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
title_full_unstemmed Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
title_sort Exploring solute binding proteins in Pseudomonas aeruginosa that bind to γ-aminobutyrate and 5-aminovalerate and their role in activating sensor kinases
dc.creator.none.fl_str_mv Cerna-Vargas, Jean Paul
Krell, Tino
author Cerna-Vargas, Jean Paul
author_facet Cerna-Vargas, Jean Paul
Krell, Tino
author_role author
author2 Krell, Tino
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Cerna-Vargas, Jean Paul [0000-0001-5489-0385]
Krell, Tino [0000-0002-9040-3166]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pseudomonas aeruginosa
Molecular recognition
Sensor histidine kinase
Signal transduction
Solute binding proteins
topic Pseudomonas aeruginosa
Molecular recognition
Sensor histidine kinase
Signal transduction
Solute binding proteins
description 17 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/359623
https://api.elsevier.com/content/abstract/scopus_id/85193922034
url http://hdl.handle.net/10261/359623
https://api.elsevier.com/content/abstract/scopus_id/85193922034
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112612GB-I00
Centro de Biotecnología y Genómica de Plantas (CBGP)
ttps://doi.org/10.1002/mbo3.1415
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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