Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences

DNA testing requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. In this paper, a simple and label-free DNA-biosensor has been investigated based on the detection of DNA hybridization on layered double hydroxide (LDH) nanomate...

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Detalles Bibliográficos
Autores: Baccar, Zouhaïr Majdi, Caballero, David, Eritja Casadellà, Ramón, Errachid, Abdelhamid
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/125320
Acceso en línea:http://hdl.handle.net/10261/125320
Access Level:acceso abierto
Palabra clave:DNA-biosensor
DNA
Layered double hydroxide
Nanomaterials
Self-assembly
Thin layers
Electrochemical impedance spectroscopy
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spelling Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequencesBaccar, Zouhaïr MajdiCaballero, DavidEritja Casadellà, RamónErrachid, AbdelhamidDNA-biosensorDNALayered double hydroxideNanomaterialsSelf-assemblyThin layersElectrochemical impedance spectroscopyDNA testing requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. In this paper, a simple and label-free DNA-biosensor has been investigated based on the detection of DNA hybridization on layered double hydroxide (LDH) nanomaterials with special emphasis on targeting long single stranded DNA sequences. First, the immobilization of a 20 bases long DNA probe on a thin layer of Mg 2AlCO 3 and Mg 3AlCO 3 LDH was studied. Then, DNA hybridization reaction was detected by means of Electrochemical Impedance Spectroscopy. The resulting biosensor showed a high sensitivity for the detection of 80 bases long DNA complementary sequences. The dynamic range was 18-270 ng/ml with a detection limit lower than 1.8 ng/ml.This work was supported by the Spanish MICINN (CTQ2010-20541), the Generalitat de Catalunya (2009/SGR/208) and CIBER-BBN. Special funds were obtained from Spanish and Tunisian government to support this work within AECID program (A/2675/05 and A/6256/06).Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/125320reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés10.1016/j.electacta.2012.04.031Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1253202026-05-22T06:33:51Z
dc.title.none.fl_str_mv Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
title Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
spellingShingle Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
Baccar, Zouhaïr Majdi
DNA-biosensor
DNA
Layered double hydroxide
Nanomaterials
Self-assembly
Thin layers
Electrochemical impedance spectroscopy
title_short Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
title_full Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
title_fullStr Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
title_full_unstemmed Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
title_sort Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences
dc.creator.none.fl_str_mv Baccar, Zouhaïr Majdi
Caballero, David
Eritja Casadellà, Ramón
Errachid, Abdelhamid
author Baccar, Zouhaïr Majdi
author_facet Baccar, Zouhaïr Majdi
Caballero, David
Eritja Casadellà, Ramón
Errachid, Abdelhamid
author_role author
author2 Caballero, David
Eritja Casadellà, Ramón
Errachid, Abdelhamid
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv DNA-biosensor
DNA
Layered double hydroxide
Nanomaterials
Self-assembly
Thin layers
Electrochemical impedance spectroscopy
topic DNA-biosensor
DNA
Layered double hydroxide
Nanomaterials
Self-assembly
Thin layers
Electrochemical impedance spectroscopy
description DNA testing requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. In this paper, a simple and label-free DNA-biosensor has been investigated based on the detection of DNA hybridization on layered double hydroxide (LDH) nanomaterials with special emphasis on targeting long single stranded DNA sequences. First, the immobilization of a 20 bases long DNA probe on a thin layer of Mg 2AlCO 3 and Mg 3AlCO 3 LDH was studied. Then, DNA hybridization reaction was detected by means of Electrochemical Impedance Spectroscopy. The resulting biosensor showed a high sensitivity for the detection of 80 bases long DNA complementary sequences. The dynamic range was 18-270 ng/ml with a detection limit lower than 1.8 ng/ml.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/125320
url http://hdl.handle.net/10261/125320
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 10.1016/j.electacta.2012.04.031
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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