Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome

Plant microbiomes are known to serve several important functions for their host, and it is therefore important to understand their composition as well as the factors that may influence these microbial communities. The microbiome of Thalassia testudinum has only recently been explored, and studies to...

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Detalhes bibliográficos
Autores: Ugarelli, Kelly, Campbell, Justin E., Rhoades, O. Kennedy, Munson, Calvin J., Altieri, Andrew H., Douglass, James G., Heck, Kenneth L. Jr, Paul, Valerie J., Barry, Savanna C., Christ, Lindsey, Fourqurean, James W., Frazer, Thomas K., Linhardt, Samantha T., Martin, Charles W., McDonald, Ashley M., Main, Vivienne A., Manuel, Sarah A., Marco-Méndez, Candela, Reynolds, Laura K, Rodríguez, Alex, Rodríguez Bravo, Lucía M., Sawall, Yvonne, Smith, Khalil, Wied, William L., Choi, Chang Jae, Stingl, Ulrich
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/387115
Acesso em linha:http://hdl.handle.net/10261/387115
https://api.elsevier.com/content/abstract/scopus_id/85186930399
Access Level:Acceso aberto
Palavra-chave:Caribbean
Thalassia
Amplicon sequencing
Core microbiome
Seagrass
Seagrass beds
Seagrass microbiome
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spelling Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiomeUgarelli, KellyCampbell, Justin E.Rhoades, O. KennedyMunson, Calvin J.Altieri, Andrew H.Douglass, James G.Heck, Kenneth L. JrPaul, Valerie J.Barry, Savanna C.Christ, LindseyFourqurean, James W.Frazer, Thomas K.Linhardt, Samantha T.Martin, Charles W.McDonald, Ashley M.Main, Vivienne A.Manuel, Sarah A.Marco-Méndez, CandelaReynolds, Laura KRodríguez, AlexRodríguez Bravo, Lucía M.Sawall, YvonneSmith, KhalilWied, William L.Choi, Chang JaeStingl, UlrichCaribbeanThalassiaAmplicon sequencingCore microbiomeSeagrassSeagrass bedsSeagrass microbiomePlant microbiomes are known to serve several important functions for their host, and it is therefore important to understand their composition as well as the factors that may influence these microbial communities. The microbiome of Thalassia testudinum has only recently been explored, and studies to-date have primarily focused on characterizing the microbiome of plants in a single region. Here, we present the first characterization of the composition of the microbial communities of T. testudinum across a wide geographical range spanning three distinct regions with varying physicochemical conditions. We collected samples of leaves, roots, sediment, and water from six sites throughout the Atlantic Ocean, Caribbean Sea, and the Gulf of Mexico. We then analyzed these samples using 16S rRNA amplicon sequencing. We found that site and region can influence the microbial communities of T. testudinum, while maintaining a plant-associated core microbiome. A comprehensive comparison of available microbial community data from T. testudinum studies determined a core microbiome composed of 14 ASVs that consisted mostly of the family Rhodobacteraceae. The most abundant genera in the microbial communities included organisms with possible plant-beneficial functions, like plant-growth promoting taxa, disease suppressing taxa, and nitrogen fixers.The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the US National Science Foundation (OCE-1737247 to JC, AA, and VP, OCE-2019022 to JC, OCE-1737144 to KH, and OCE-1737116 to JD).Peer reviewedFrontiers MediaNational Science Foundation (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/387115https://api.elsevier.com/content/abstract/scopus_id/85186930399reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3389/fmicb.2024.1357797Ugarelli, Kelly; Campbell, Justin E.; Rhoades, O. Kennedy; Munson, Calvin J.; Altieri, Andrew H.; Douglass, James G.; Heck, Kenneth L. Jr; Paul, Valerie J.; Barry, Savanna C.; Christ, Lindsey; Fourqurean, James W.; Frazer, Thomas K.; Linhardt, Samantha T.; Martin, Charles W.; McDonald, Ashley M.; Main, Vivienne A.; Manuel, Sarah A.; Marco-Méndez, Candela; Reynolds, Laura K; Rodríguez, Alex; Rodríguez Bravo, Lucía M.; Sawall, Yvonne; Smith, Khalil; Wied, William L.; Choi, Chang Jae; Stingl, Ulrich; 2024; Supplementary material: Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome; Frontiers Media; https://doi.org/10.3389/fmicb.2024.1357797https://doi.org/10.3389/fmicb.2024.1357797Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3871152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
title Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
spellingShingle Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
Ugarelli, Kelly
Caribbean
Thalassia
Amplicon sequencing
Core microbiome
Seagrass
Seagrass beds
Seagrass microbiome
title_short Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
title_full Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
title_fullStr Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
title_full_unstemmed Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
title_sort Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome
dc.creator.none.fl_str_mv Ugarelli, Kelly
Campbell, Justin E.
Rhoades, O. Kennedy
Munson, Calvin J.
Altieri, Andrew H.
Douglass, James G.
Heck, Kenneth L. Jr
Paul, Valerie J.
Barry, Savanna C.
Christ, Lindsey
Fourqurean, James W.
Frazer, Thomas K.
Linhardt, Samantha T.
Martin, Charles W.
McDonald, Ashley M.
Main, Vivienne A.
Manuel, Sarah A.
Marco-Méndez, Candela
Reynolds, Laura K
Rodríguez, Alex
Rodríguez Bravo, Lucía M.
Sawall, Yvonne
Smith, Khalil
Wied, William L.
Choi, Chang Jae
Stingl, Ulrich
author Ugarelli, Kelly
author_facet Ugarelli, Kelly
Campbell, Justin E.
Rhoades, O. Kennedy
Munson, Calvin J.
Altieri, Andrew H.
Douglass, James G.
Heck, Kenneth L. Jr
Paul, Valerie J.
Barry, Savanna C.
Christ, Lindsey
Fourqurean, James W.
Frazer, Thomas K.
Linhardt, Samantha T.
Martin, Charles W.
McDonald, Ashley M.
Main, Vivienne A.
Manuel, Sarah A.
Marco-Méndez, Candela
Reynolds, Laura K
Rodríguez, Alex
Rodríguez Bravo, Lucía M.
Sawall, Yvonne
Smith, Khalil
Wied, William L.
Choi, Chang Jae
Stingl, Ulrich
author_role author
author2 Campbell, Justin E.
Rhoades, O. Kennedy
Munson, Calvin J.
Altieri, Andrew H.
Douglass, James G.
Heck, Kenneth L. Jr
Paul, Valerie J.
Barry, Savanna C.
Christ, Lindsey
Fourqurean, James W.
Frazer, Thomas K.
Linhardt, Samantha T.
Martin, Charles W.
McDonald, Ashley M.
Main, Vivienne A.
Manuel, Sarah A.
Marco-Méndez, Candela
Reynolds, Laura K
Rodríguez, Alex
Rodríguez Bravo, Lucía M.
Sawall, Yvonne
Smith, Khalil
Wied, William L.
Choi, Chang Jae
Stingl, Ulrich
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Science Foundation (US)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Caribbean
Thalassia
Amplicon sequencing
Core microbiome
Seagrass
Seagrass beds
Seagrass microbiome
topic Caribbean
Thalassia
Amplicon sequencing
Core microbiome
Seagrass
Seagrass beds
Seagrass microbiome
description Plant microbiomes are known to serve several important functions for their host, and it is therefore important to understand their composition as well as the factors that may influence these microbial communities. The microbiome of Thalassia testudinum has only recently been explored, and studies to-date have primarily focused on characterizing the microbiome of plants in a single region. Here, we present the first characterization of the composition of the microbial communities of T. testudinum across a wide geographical range spanning three distinct regions with varying physicochemical conditions. We collected samples of leaves, roots, sediment, and water from six sites throughout the Atlantic Ocean, Caribbean Sea, and the Gulf of Mexico. We then analyzed these samples using 16S rRNA amplicon sequencing. We found that site and region can influence the microbial communities of T. testudinum, while maintaining a plant-associated core microbiome. A comprehensive comparison of available microbial community data from T. testudinum studies determined a core microbiome composed of 14 ASVs that consisted mostly of the family Rhodobacteraceae. The most abundant genera in the microbial communities included organisms with possible plant-beneficial functions, like plant-growth promoting taxa, disease suppressing taxa, and nitrogen fixers.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/387115
https://api.elsevier.com/content/abstract/scopus_id/85186930399
url http://hdl.handle.net/10261/387115
https://api.elsevier.com/content/abstract/scopus_id/85186930399
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3389/fmicb.2024.1357797
Ugarelli, Kelly; Campbell, Justin E.; Rhoades, O. Kennedy; Munson, Calvin J.; Altieri, Andrew H.; Douglass, James G.; Heck, Kenneth L. Jr; Paul, Valerie J.; Barry, Savanna C.; Christ, Lindsey; Fourqurean, James W.; Frazer, Thomas K.; Linhardt, Samantha T.; Martin, Charles W.; McDonald, Ashley M.; Main, Vivienne A.; Manuel, Sarah A.; Marco-Méndez, Candela; Reynolds, Laura K; Rodríguez, Alex; Rodríguez Bravo, Lucía M.; Sawall, Yvonne; Smith, Khalil; Wied, William L.; Choi, Chang Jae; Stingl, Ulrich; 2024; Supplementary material: Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome; Frontiers Media; https://doi.org/10.3389/fmicb.2024.1357797
https://doi.org/10.3389/fmicb.2024.1357797

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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
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instname:Consejo Superior de Investigaciones Científicas (CSIC)
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