Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial

Background Early branching or syllepsis has been positively correlated with high biomass yields in short-rotation coppice (SRC) poplar plantations, which could represent an important lignocellulosic feedstock for the production of second-generation bioenergy. In prior work, we generated hybrid popla...

Descripción completa

Detalles Bibliográficos
Autores: Moreno-Cortés, A., Ramos-Sánchez, J. M., Hernández-Verdeja, Tamara, Gonzalez-Melendi, P., Alves, Ana, Simões, Rita, Rodrigues, José Carlos, Guijarro Guzmán, Mercedes, Cañellas, Isabel, Sixto Blanco, Hortensia Concepción, Allona, Isabel
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/293839
Acceso en línea:http://hdl.handle.net/10261/293839
Access Level:acceso abierto
Palabra clave:Poplar
Tree biotechnology
RAV1
Sylleptic branching
Sylleptic branchiness
Lignocellulosic biomass
Field trial
Short-rotation coppice (SRC)
TEM1
id ES_2ca65d1bbd9d48fbd4e5a0499fe7dbfe
oai_identifier_str oai:digital.csic.es:10261/293839
network_acronym_str ES
network_name_str España
repository_id_str
spelling Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trialMoreno-Cortés, A.Ramos-Sánchez, J. M.Hernández-Verdeja, TamaraGonzalez-Melendi, P.Alves, AnaSimões, RitaRodrigues, José CarlosGuijarro Guzmán, MercedesCañellas, IsabelSixto Blanco, Hortensia ConcepciónAllona, IsabelPoplarTree biotechnologyRAV1Sylleptic branchingSylleptic branchinessLignocellulosic biomassField trialShort-rotation coppice (SRC)TEM1Background Early branching or syllepsis has been positively correlated with high biomass yields in short-rotation coppice (SRC) poplar plantations, which could represent an important lignocellulosic feedstock for the production of second-generation bioenergy. In prior work, we generated hybrid poplars overexpressing the chestnut gene RELATED TO ABI3/VP1 1 (CsRAV1), which featured c. 80% more sylleptic branches than non-modified trees in growth chambers. Given the high plasticity of syllepsis, we established a field trial to monitor the performance of these trees under outdoor conditions and a SRC management. Results We examined two CsRAV1-overexpression poplar events for their ability to maintain syllepsis and their potential to enhance biomass production. Two poplar events with reduced expression of the CsRAV1 homologous poplar genes PtaRAV1 and PtaRAV2 were also included in the trial. Under our culture conditions, CsRAV1-overexpression poplars continued developing syllepsis over two cultivation cycles. Biomass production increased on completion of the first cycle for one of the overexpression events, showing unaltered structural, chemical, or combustion wood properties. On completion of the second cycle, aerial growth and biomass yields of both overexpression events were reduced as compared to the control. Conclusions These findings support the potential application of CsRAV1-overexpression to increase syllepsis in commercial elite trees without changing their wood quality. However, the syllepsis triggered by the introduction of this genetic modification appeared not to be sufficient to sustain and enhance biomass production. © 2017 The Author(s).Peer reviewedBioMed CentralHernández-Verdeja, Tamara [0000-0002-2148-3676]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/293839reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro de Biotecnología y Genómica de Plantas (CBGP)Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2938392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
title Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
spellingShingle Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
Moreno-Cortés, A.
Poplar
Tree biotechnology
RAV1
Sylleptic branching
Sylleptic branchiness
Lignocellulosic biomass
Field trial
Short-rotation coppice (SRC)
TEM1
title_short Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
title_full Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
title_fullStr Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
title_full_unstemmed Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
title_sort Impact of RAV1-engineering on poplar biomass production A short-rotation coppice field trial
dc.creator.none.fl_str_mv Moreno-Cortés, A.
Ramos-Sánchez, J. M.
Hernández-Verdeja, Tamara
Gonzalez-Melendi, P.
Alves, Ana
Simões, Rita
Rodrigues, José Carlos
Guijarro Guzmán, Mercedes
Cañellas, Isabel
Sixto Blanco, Hortensia Concepción
Allona, Isabel
author Moreno-Cortés, A.
author_facet Moreno-Cortés, A.
Ramos-Sánchez, J. M.
Hernández-Verdeja, Tamara
Gonzalez-Melendi, P.
Alves, Ana
Simões, Rita
Rodrigues, José Carlos
Guijarro Guzmán, Mercedes
Cañellas, Isabel
Sixto Blanco, Hortensia Concepción
Allona, Isabel
author_role author
author2 Ramos-Sánchez, J. M.
Hernández-Verdeja, Tamara
Gonzalez-Melendi, P.
Alves, Ana
Simões, Rita
Rodrigues, José Carlos
Guijarro Guzmán, Mercedes
Cañellas, Isabel
Sixto Blanco, Hortensia Concepción
Allona, Isabel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Hernández-Verdeja, Tamara [0000-0002-2148-3676]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Poplar
Tree biotechnology
RAV1
Sylleptic branching
Sylleptic branchiness
Lignocellulosic biomass
Field trial
Short-rotation coppice (SRC)
TEM1
topic Poplar
Tree biotechnology
RAV1
Sylleptic branching
Sylleptic branchiness
Lignocellulosic biomass
Field trial
Short-rotation coppice (SRC)
TEM1
description Background Early branching or syllepsis has been positively correlated with high biomass yields in short-rotation coppice (SRC) poplar plantations, which could represent an important lignocellulosic feedstock for the production of second-generation bioenergy. In prior work, we generated hybrid poplars overexpressing the chestnut gene RELATED TO ABI3/VP1 1 (CsRAV1), which featured c. 80% more sylleptic branches than non-modified trees in growth chambers. Given the high plasticity of syllepsis, we established a field trial to monitor the performance of these trees under outdoor conditions and a SRC management. Results We examined two CsRAV1-overexpression poplar events for their ability to maintain syllepsis and their potential to enhance biomass production. Two poplar events with reduced expression of the CsRAV1 homologous poplar genes PtaRAV1 and PtaRAV2 were also included in the trial. Under our culture conditions, CsRAV1-overexpression poplars continued developing syllepsis over two cultivation cycles. Biomass production increased on completion of the first cycle for one of the overexpression events, showing unaltered structural, chemical, or combustion wood properties. On completion of the second cycle, aerial growth and biomass yields of both overexpression events were reduced as compared to the control. Conclusions These findings support the potential application of CsRAV1-overexpression to increase syllepsis in commercial elite trees without changing their wood quality. However, the syllepsis triggered by the introduction of this genetic modification appeared not to be sufficient to sustain and enhance biomass production. © 2017 The Author(s).
publishDate 2017
dc.date.none.fl_str_mv 2017
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/293839
url http://hdl.handle.net/10261/293839
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro de Biotecnología y Genómica de Plantas (CBGP)
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405255891943424
score 15,81155

Similares en LA Referencia