Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds

Europium is one of the most reactive lanthanides and humans use it in many different applications, but we still know little about its potential toxicity and cellular response to its exposure. Two strains of the eukaryotic microorganism model Tetrahymena thermophila were adapted to high concentration...

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Detalles Bibliográficos
Autores: Alonso, Patricia, Blas, Javier, Amaro Torres, Francisco, De Francisco Martínez, Patricia, Martín González, Ana María, Gutiérrez Fernández, Juan Carlos
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103968
Acceso en línea:https://hdl.handle.net/20.500.14352/103968
Access Level:acceso abierto
Palabra clave:579
Europium
Lipid metabolism
Bioaccumulation
Oxidative stress
Gene expression
Tetrahymena thermophila
Microbiología (Biología)
2407 Biología Celular
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oai_identifier_str oai:docta.ucm.es:20.500.14352/103968
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repository_id_str
spelling Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compoundsRespuesta celular de cepas de Tetrahymena thermophila adaptadas y no adaptadas a compuesto de europio Eu (III).Alonso, PatriciaBlas, JavierAmaro Torres, FranciscoDe Francisco Martínez, PatriciaMartín González, Ana MaríaGutiérrez Fernández, Juan Carlos579EuropiumLipid metabolismBioaccumulationOxidative stressGene expressionTetrahymena thermophilaMicrobiología (Biología)2407 Biología CelularEuropium is one of the most reactive lanthanides and humans use it in many different applications, but we still know little about its potential toxicity and cellular response to its exposure. Two strains of the eukaryotic microorganism model Tetrahymena thermophila were adapted to high concentrations of two Eu(III) compounds (EuCl3 or Eu2O3) and compared to a control strain and cultures treated with both compounds. In this ciliate, EuCl3 is more toxic than Eu2O3. LC50 values show that this microorganism is more resistant to these Eu(III) compounds than other microorganisms. Oxidative stress originated mainly by Eu2O3 is minimized by overexpression of genes encoding important antioxidant enzymes. The overexpression of metallothionein genes under treatment with Eu(III) compounds supports the possibility that this lanthanide may interact with the -SH groups of the cysteine residues from metallothioneins and/or displace essential cations of these proteins during their homeostatic function. Both lipid metabolism (lipid droplets fusing with europium-containing vacuoles) and autophagy are involved in the cellular response to europium stress. Bioaccumulation, together with a possible biomineralization to europium phosphate, seems to be the main mechanism of Eu(III) detoxification in these cells.MDPIUniversidad Complutense de Madrid20242024-04-2320242024-04-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103968reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Educación y Ciencia Not available CGL2016-75494-Ropen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1039682026-06-02T12:44:21Z
dc.title.none.fl_str_mv Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
Respuesta celular de cepas de Tetrahymena thermophila adaptadas y no adaptadas a compuesto de europio Eu (III).
title Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
spellingShingle Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
Alonso, Patricia
579
Europium
Lipid metabolism
Bioaccumulation
Oxidative stress
Gene expression
Tetrahymena thermophila
Microbiología (Biología)
2407 Biología Celular
title_short Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
title_full Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
title_fullStr Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
title_full_unstemmed Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
title_sort Cellular response of adapted and non-adapted Tetrahymena thermophila strains to europium Eu(III) compounds
dc.creator.none.fl_str_mv Alonso, Patricia
Blas, Javier
Amaro Torres, Francisco
De Francisco Martínez, Patricia
Martín González, Ana María
Gutiérrez Fernández, Juan Carlos
author Alonso, Patricia
author_facet Alonso, Patricia
Blas, Javier
Amaro Torres, Francisco
De Francisco Martínez, Patricia
Martín González, Ana María
Gutiérrez Fernández, Juan Carlos
author_role author
author2 Blas, Javier
Amaro Torres, Francisco
De Francisco Martínez, Patricia
Martín González, Ana María
Gutiérrez Fernández, Juan Carlos
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
Europium
Lipid metabolism
Bioaccumulation
Oxidative stress
Gene expression
Tetrahymena thermophila
Microbiología (Biología)
2407 Biología Celular
topic 579
Europium
Lipid metabolism
Bioaccumulation
Oxidative stress
Gene expression
Tetrahymena thermophila
Microbiología (Biología)
2407 Biología Celular
description Europium is one of the most reactive lanthanides and humans use it in many different applications, but we still know little about its potential toxicity and cellular response to its exposure. Two strains of the eukaryotic microorganism model Tetrahymena thermophila were adapted to high concentrations of two Eu(III) compounds (EuCl3 or Eu2O3) and compared to a control strain and cultures treated with both compounds. In this ciliate, EuCl3 is more toxic than Eu2O3. LC50 values show that this microorganism is more resistant to these Eu(III) compounds than other microorganisms. Oxidative stress originated mainly by Eu2O3 is minimized by overexpression of genes encoding important antioxidant enzymes. The overexpression of metallothionein genes under treatment with Eu(III) compounds supports the possibility that this lanthanide may interact with the -SH groups of the cysteine residues from metallothioneins and/or displace essential cations of these proteins during their homeostatic function. Both lipid metabolism (lipid droplets fusing with europium-containing vacuoles) and autophagy are involved in the cellular response to europium stress. Bioaccumulation, together with a possible biomineralization to europium phosphate, seems to be the main mechanism of Eu(III) detoxification in these cells.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-23
2024
2024-04-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103968
url https://hdl.handle.net/20.500.14352/103968
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Educación y Ciencia Not available CGL2016-75494-R
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405245517332480
score 15.300724

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