PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts

We isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs from corresponding synchronous liver metastasis (CAFlm) as well as normal colonic fibroblasts (NCF) from the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide pane...

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Detalhes bibliográficos
Autores: Garcia i Molleví, David, Aytés Meneses, Álvaro, Berdiel Acer, Mireia, Padullés Mosella, Laura, Martínez Iniesta, María, Sanjuan, Xavier, Salazar Soler, Ramón, Villanueva Garatachea, Alberto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/125936
Acesso em linha:https://hdl.handle.net/2445/125936
Access Level:acceso abierto
Palavra-chave:Càncer
Fibroblasts
Metàstasi
Cancer
Metastasis
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repository_id_str
spelling PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblastsGarcia i Molleví, DavidAytés Meneses, ÁlvaroBerdiel Acer, MireiaPadullés Mosella, LauraMartínez Iniesta, MaríaSanjuan, XavierSalazar Soler, RamónVillanueva Garatachea, AlbertoCàncerFibroblastsMetàstasiCancerFibroblastsMetastasisWe isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs from corresponding synchronous liver metastasis (CAFlm) as well as normal colonic fibroblasts (NCF) from the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide panel of colorectal and pancreatic cell lines in CM from CAFlm resulted in overexpression of mRNA PRL-3 and higher overexpression in CAFs than in non-activated fibroblasts. Moreover PRL-3 mRNA expression correlates with expression of alpha-SMA and deposition of collagen fibrils in the stroma. We demonstrate that products secreted by CAFs trigger PRL-3 overexpression in cancer cells. Identification of these factors may contribute to new stroma-targeted therapies for desmoplastic tumoursBioMed Central2018201820092018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/125936Articles publicats en revistes (Ciències Clíniques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1186/1476-4598-8-46Molecular Cancer, 2009, vol. 8, p. 46https://doi.org/10.1186/1476-4598-8-46cc-by (c) Garcia i Molleví, David et al., 2009http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1259362026-05-29T05:05:01Z
dc.title.none.fl_str_mv PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
title PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
spellingShingle PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
Garcia i Molleví, David
Càncer
Fibroblasts
Metàstasi
Cancer
Fibroblasts
Metastasis
title_short PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
title_full PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
title_fullStr PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
title_full_unstemmed PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
title_sort PRL-3 overexpression in epithelial cells is induced by surrounding stromal fibroblasts
dc.creator.none.fl_str_mv Garcia i Molleví, David
Aytés Meneses, Álvaro
Berdiel Acer, Mireia
Padullés Mosella, Laura
Martínez Iniesta, María
Sanjuan, Xavier
Salazar Soler, Ramón
Villanueva Garatachea, Alberto
author Garcia i Molleví, David
author_facet Garcia i Molleví, David
Aytés Meneses, Álvaro
Berdiel Acer, Mireia
Padullés Mosella, Laura
Martínez Iniesta, María
Sanjuan, Xavier
Salazar Soler, Ramón
Villanueva Garatachea, Alberto
author_role author
author2 Aytés Meneses, Álvaro
Berdiel Acer, Mireia
Padullés Mosella, Laura
Martínez Iniesta, María
Sanjuan, Xavier
Salazar Soler, Ramón
Villanueva Garatachea, Alberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer
Fibroblasts
Metàstasi
Cancer
Fibroblasts
Metastasis
topic Càncer
Fibroblasts
Metàstasi
Cancer
Fibroblasts
Metastasis
description We isolate and culture carcinoma-associated fibroblasts (CAFs) from primary tumour (CAFpt), CAFs from corresponding synchronous liver metastasis (CAFlm) as well as normal colonic fibroblasts (NCF) from the same patient. From these cultures, conditioned media (CM) was obtained. Culture of a wide panel of colorectal and pancreatic cell lines in CM from CAFlm resulted in overexpression of mRNA PRL-3 and higher overexpression in CAFs than in non-activated fibroblasts. Moreover PRL-3 mRNA expression correlates with expression of alpha-SMA and deposition of collagen fibrils in the stroma. We demonstrate that products secreted by CAFs trigger PRL-3 overexpression in cancer cells. Identification of these factors may contribute to new stroma-targeted therapies for desmoplastic tumours
publishDate 2009
dc.date.none.fl_str_mv 2009
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/125936
url https://hdl.handle.net/2445/125936
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/1476-4598-8-46
Molecular Cancer, 2009, vol. 8, p. 46
https://doi.org/10.1186/1476-4598-8-46
dc.rights.none.fl_str_mv cc-by (c) Garcia i Molleví, David et al., 2009
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Garcia i Molleví, David et al., 2009
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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