Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells

Brunfelsia grandiflora is a South American solanaceae widely used since long ago for its recognized medicinal properties. We have recently reported its chemical composition, showing a relevant number of bioactive compounds with antioxidant capacity, and proved the cytoprotective and antioxidative st...

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Autores: Rodríguez, José-Luis, Mateos, Raquel, Palomino, Olga, Fernández-Alfonso, María Soledad, Ramos-Cevallos, Norma, Inostroza-Ruiz, Luis, Panduro-Tenazoa, Nadia, Bada-Laura, Wendy, Ramírez-Flores, Noé, Goya, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/340172
Acceso en línea:http://hdl.handle.net/10261/340172
Access Level:acceso abierto
Palabra clave:Brunfelsia grandiflora
Antioxidative stress
Neuronal cells
Cytoprotection
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spelling Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like CellsRodríguez, José-LuisMateos, RaquelPalomino, OlgaFernández-Alfonso, María SoledadRamos-Cevallos, NormaInostroza-Ruiz, LuisPanduro-Tenazoa, NadiaBada-Laura, WendyRamírez-Flores, NoéGoya, LuisBrunfelsia grandifloraAntioxidative stressNeuronal cellsCytoprotectionBrunfelsia grandiflora is a South American solanaceae widely used since long ago for its recognized medicinal properties. We have recently reported its chemical composition, showing a relevant number of bioactive compounds with antioxidant capacity, and proved the cytoprotective and antioxidative stress potential of B. grandiflora in cultured endothelial cells. Since B. grandiflora extracts have shown effects on the central nervous system, the present study was designed to show the potential cytoprotective capacity and the antioxidative stress potential of phenolic extracts from the plant on cultured neuron-like cells, as a model to reduce the presentation or effects of chronic diseases of the nervous system. To this end, we studied its reactive oxygen species (ROS)-reducing capacity, its antioxidant defense mechanisms, and some molecular markers involved in redox balance and apoptosis. The results show that cell survival and most changes in biomarkers related to oxidative status, ROS, reduced glutathione, glutathione peroxidase and reductase, malondialdehyde, and caspase 3/7 activity, and molecular expression of cell death-related genes (BAX, BNIP3, and APAF1), NFκB, SOD, and NRF2 (genes from oxidative stress—antioxidants) induced by oxidative stress were prevented by either co- or pretreatment of neuron-like cells with B. grandiflora extracts (25–200 µg/mL). The results demonstrate the chemoprotective potential of the plant and support its medicinal use.Ministry of Economy, Industry and Competitiveness, BFU2017-82565-C2-2-R. National University of San Marcos, PCONFIGI A23040181.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Ministerio de Economía, Industria y Competitividad (España)Universidad Nacional Mayor de San MarcosConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/340172reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-82565-C2-2-RRodríguez, José-Luis; Mateos, Raquel; Palomino, Olga; Fernández-Alfonso, Maria S.; Ramos-Cevallos, Norma; Inostroza-Ruiz, Luis; Panduro-Tenazoa, Nadia; Bada-Laura, Wendy; Ramírez-Flores, Noé; Goya, Luis; 2023; Supplementary Materials: Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells [Dataset]; Multidisciplinary Digital Publishing Institute; https://doi.org/10.3390/app132212233https://doi.org/10.3390/app132212233Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3401722026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
title Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
spellingShingle Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
Rodríguez, José-Luis
Brunfelsia grandiflora
Antioxidative stress
Neuronal cells
Cytoprotection
title_short Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
title_full Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
title_fullStr Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
title_full_unstemmed Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
title_sort Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells
dc.creator.none.fl_str_mv Rodríguez, José-Luis
Mateos, Raquel
Palomino, Olga
Fernández-Alfonso, María Soledad
Ramos-Cevallos, Norma
Inostroza-Ruiz, Luis
Panduro-Tenazoa, Nadia
Bada-Laura, Wendy
Ramírez-Flores, Noé
Goya, Luis
author Rodríguez, José-Luis
author_facet Rodríguez, José-Luis
Mateos, Raquel
Palomino, Olga
Fernández-Alfonso, María Soledad
Ramos-Cevallos, Norma
Inostroza-Ruiz, Luis
Panduro-Tenazoa, Nadia
Bada-Laura, Wendy
Ramírez-Flores, Noé
Goya, Luis
author_role author
author2 Mateos, Raquel
Palomino, Olga
Fernández-Alfonso, María Soledad
Ramos-Cevallos, Norma
Inostroza-Ruiz, Luis
Panduro-Tenazoa, Nadia
Bada-Laura, Wendy
Ramírez-Flores, Noé
Goya, Luis
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Ministerio de Economía, Industria y Competitividad (España)
Universidad Nacional Mayor de San Marcos
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brunfelsia grandiflora
Antioxidative stress
Neuronal cells
Cytoprotection
topic Brunfelsia grandiflora
Antioxidative stress
Neuronal cells
Cytoprotection
description Brunfelsia grandiflora is a South American solanaceae widely used since long ago for its recognized medicinal properties. We have recently reported its chemical composition, showing a relevant number of bioactive compounds with antioxidant capacity, and proved the cytoprotective and antioxidative stress potential of B. grandiflora in cultured endothelial cells. Since B. grandiflora extracts have shown effects on the central nervous system, the present study was designed to show the potential cytoprotective capacity and the antioxidative stress potential of phenolic extracts from the plant on cultured neuron-like cells, as a model to reduce the presentation or effects of chronic diseases of the nervous system. To this end, we studied its reactive oxygen species (ROS)-reducing capacity, its antioxidant defense mechanisms, and some molecular markers involved in redox balance and apoptosis. The results show that cell survival and most changes in biomarkers related to oxidative status, ROS, reduced glutathione, glutathione peroxidase and reductase, malondialdehyde, and caspase 3/7 activity, and molecular expression of cell death-related genes (BAX, BNIP3, and APAF1), NFκB, SOD, and NRF2 (genes from oxidative stress—antioxidants) induced by oxidative stress were prevented by either co- or pretreatment of neuron-like cells with B. grandiflora extracts (25–200 µg/mL). The results demonstrate the chemoprotective potential of the plant and support its medicinal use.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/340172
url http://hdl.handle.net/10261/340172
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-82565-C2-2-R
Rodríguez, José-Luis; Mateos, Raquel; Palomino, Olga; Fernández-Alfonso, Maria S.; Ramos-Cevallos, Norma; Inostroza-Ruiz, Luis; Panduro-Tenazoa, Nadia; Bada-Laura, Wendy; Ramírez-Flores, Noé; Goya, Luis; 2023; Supplementary Materials: Cytoprotective–Antioxidant Effect of Brunfelsia grandiflora Extract on Neuron-like Cells [Dataset]; Multidisciplinary Digital Publishing Institute; https://doi.org/10.3390/app132212233
https://doi.org/10.3390/app132212233
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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