Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments

The air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microsco...

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Detalles Bibliográficos
Autores: Núñez, Andrés, Amo de Paz, Guillermo, Rastrojo, Alberto, García, Ana M., Alcamí, Antonio, Gutiérrez-Bustillo, A. Montserrat, Moreno, Diego A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/151757
Acceso en línea:http://hdl.handle.net/10261/151757
Access Level:acceso abierto
Palabra clave:Metagenomics
Next-generation sequencing
Air biomonitoring
Urban aerobiology
Airborne biological particles
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spelling Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environmentsNúñez, AndrésAmo de Paz, GuillermoRastrojo, AlbertoGarcía, Ana M.Alcamí, AntonioGutiérrez-Bustillo, A. MontserratMoreno, Diego A.MetagenomicsNext-generation sequencingAir biomonitoringUrban aerobiologyAirborne biological particlesThe air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete. Currently, metagenomics and next-generation sequencing (NGS) may resolve this shortage of information and have been recently applied to metropolitan areas. Although the procedures and methods are not totally standardized yet, the first studies from urban air samples confirm the previous results obtained by culture and microscopy regarding abundance and variation of these biological particles. However, DNA-sequence analyses call into question some preceding ideas and also provide new interesting insights into diversity and their spatial distribution inside the cities. Here, we review the procedures, results and perspectives of the recent works that apply NGS to study the main biological particles present in the air of urban environments.Community of Madrid, Spain, under the AIRBIOTA-CM Program (S2013/MAE-2874)Peer ReviewedSociedad Española de MicrobiologíaComunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/151757reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1517572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
title Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
spellingShingle Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
Núñez, Andrés
Metagenomics
Next-generation sequencing
Air biomonitoring
Urban aerobiology
Airborne biological particles
title_short Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
title_full Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
title_fullStr Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
title_full_unstemmed Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
title_sort Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
dc.creator.none.fl_str_mv Núñez, Andrés
Amo de Paz, Guillermo
Rastrojo, Alberto
García, Ana M.
Alcamí, Antonio
Gutiérrez-Bustillo, A. Montserrat
Moreno, Diego A.
author Núñez, Andrés
author_facet Núñez, Andrés
Amo de Paz, Guillermo
Rastrojo, Alberto
García, Ana M.
Alcamí, Antonio
Gutiérrez-Bustillo, A. Montserrat
Moreno, Diego A.
author_role author
author2 Amo de Paz, Guillermo
Rastrojo, Alberto
García, Ana M.
Alcamí, Antonio
Gutiérrez-Bustillo, A. Montserrat
Moreno, Diego A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Metagenomics
Next-generation sequencing
Air biomonitoring
Urban aerobiology
Airborne biological particles
topic Metagenomics
Next-generation sequencing
Air biomonitoring
Urban aerobiology
Airborne biological particles
description The air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete. Currently, metagenomics and next-generation sequencing (NGS) may resolve this shortage of information and have been recently applied to metropolitan areas. Although the procedures and methods are not totally standardized yet, the first studies from urban air samples confirm the previous results obtained by culture and microscopy regarding abundance and variation of these biological particles. However, DNA-sequence analyses call into question some preceding ideas and also provide new interesting insights into diversity and their spatial distribution inside the cities. Here, we review the procedures, results and perspectives of the recent works that apply NGS to study the main biological particles present in the air of urban environments.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/151757
url http://hdl.handle.net/10261/151757
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedad Española de Microbiología
publisher.none.fl_str_mv Sociedad Española de Microbiología
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405205671444480
score 15,811543

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