Hetero-Diels-Alder Cycloaddition with RAFT Polymers as Bioconjugation Platform

We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero-Diels-Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein. Quantitative conjugation occurs over the...

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Detalles Bibliográficos
Autores: Beloqui Elizazu, Ana, Mane, Shivshankar R., Langer, Marcel, Glassner, Mathias, Bauer, Dennis M., Fruk, Ljiljana, Barner-Kowollik, Christopher, Delaittre, Guillaume
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/50462
Acceso en línea:http://hdl.handle.net/10810/50462
Access Level:acceso abierto
Palabra clave:bioconjugation
Diels-Alder cycloaddition
end group
polymer
protein
conjugation
stability
glycol
well
polymerization
copolymers
chemistry
behavior
design
drug
Descripción
Sumario:We introduce the bioconjugation of polymers synthesized by RAFT polymerization, bearing no specific functional end group, by means of hetero-Diels-Alder cycloaddition through their inherent terminal thiocarbonylthio moiety with a diene-modified model protein. Quantitative conjugation occurs over the course of a few hours, at ambient temperature and neutral pH, and in the absence of any catalyst. Our technology platform affords thermoresponsive bioconjugates, whose aggregation is solely controlled by the polymer chains.