The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe

Wallace, Megan A.; Coffman, Kelsey A.; Gilbert, Clément; Ravindran, Sanjana; Albery, Gregory F.; Abbott, Jessica; Argyridou, Eliza; Bellosta, Paola; Betancourt, Andrea J.; Colinet, Hervé; Eric, Katarina; Glaser-Schmitt, Amanda; Grath, Sonja; Jelic, Mihailo; Kankare, Maaria; Kozeretska, Iryna; Loeschcke, Volker; Montchamp-Moreau, Catherine; Ometto, Lino; Onder, Banu Sebnem; Orengo Ferriz, Dorcas J.; Parsch, John; Pascual Berniola, Marta; Patenkovic, Aleksandra; Puerma Rodríguez, Eva María; Ritchie, Michael G.; Rota-Stabelli, Omar; Schou, Mads Fristrup; Serga, Svitlana; Stamenkovic-Radak, Marina; Tanaskovic, Marija; Savic Veselinovic, Marija; Vieira, Jorge; Vieira, Cristina P.; Kapun, Martin; Flatt, Thomas; González, Josefa; Staubach, Fabian; Obbard, Darren J.

The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe

Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This has limited our opportunity to use natural host-pathogen combinations in experimental studies, and may have biased our understanding of...

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Autores:	Wallace, Megan A., Coffman, Kelsey A., Gilbert, Clément, Ravindran, Sanjana, Albery, Gregory F., Abbott, Jessica, Argyridou, Eliza, Bellosta, Paola, Betancourt, Andrea J., Colinet, Hervé, Eric, Katarina, Glaser-Schmitt, Amanda, Grath, Sonja, Jelic, Mihailo, Kankare, Maaria, Kozeretska, Iryna, Loeschcke, Volker, Montchamp-Moreau, Catherine, Ometto, Lino, Onder, Banu Sebnem, Orengo Ferriz, Dorcas J., Parsch, John, Pascual Berniola, Marta, Patenkovic, Aleksandra, Puerma Rodríguez, Eva María, Ritchie, Michael G., Rota-Stabelli, Omar, Schou, Mads Fristrup, Serga, Svitlana, Stamenkovic-Radak, Marina, Tanaskovic, Marija, Savic Veselinovic, Marija, Vieira, Jorge, Vieira, Cristina P., Kapun, Martin, Flatt, Thomas, González, Josefa, Staubach, Fabian, Obbard, Darren J.
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2021
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/197754
Acceso en línea:	https://hdl.handle.net/2445/197754
Access Level:	acceso abierto
Palabra clave:	Virus ADN Drosòfila DNA viruses Drosophila

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spelling	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in EuropeWallace, Megan A.Coffman, Kelsey A.Gilbert, ClémentRavindran, SanjanaAlbery, Gregory F.Abbott, JessicaArgyridou, ElizaBellosta, PaolaBetancourt, Andrea J.Colinet, HervéEric, KatarinaGlaser-Schmitt, AmandaGrath, SonjaJelic, MihailoKankare, MaariaKozeretska, IrynaLoeschcke, VolkerMontchamp-Moreau, CatherineOmetto, LinoOnder, Banu SebnemOrengo Ferriz, Dorcas J.Parsch, JohnPascual Berniola, MartaPatenkovic, AleksandraPuerma Rodríguez, Eva MaríaRitchie, Michael G.Rota-Stabelli, OmarSchou, Mads FristrupSerga, SvitlanaStamenkovic-Radak, MarinaTanaskovic, MarijaSavic Veselinovic, MarijaVieira, JorgeVieira, Cristina P.Kapun, MartinFlatt, ThomasGonzález, JosefaStaubach, FabianObbard, Darren J.Virus ADNDrosòfilaDNA virusesDrosophilaDrosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This has limited our opportunity to use natural host-pathogen combinations in experimental studies, and may have biased our understanding of the Drosophila virome. Here we report fourteen DNA viruses detectable by metagenomic analysis of six and a half thousand pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new Nudiviruses, a new and divergent Entomopox virus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of Galbut virus, a dsRNA Partitivirus, segregating at very low frequency. Remarkably, we show that Drosophila Vesanto virus, a small DNA virus previously described as a Bidnavirus, may be composed of up to 12 segments and represent a new lineage of segmented DNA viruses. Only two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, being found in 2% or more of wild flies. The other viruses are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus only being commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are generally at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redress an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.Oxford University Press2023202320212023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion23 p.application/pdfhttps://hdl.handle.net/2445/197754Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1093/ve/veab031Virus Evolution, 2021, vol. 7, num. 1, p. 1-23https://doi.org/10.1093/ve/veab031cc-by (c) Wallace, Megan A. et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1977542026-05-29T05:05:01Z
dc.title.none.fl_str_mv	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
spellingShingle	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe Wallace, Megan A. Virus ADN Drosòfila DNA viruses Drosophila
title_short	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_full	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_fullStr	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_full_unstemmed	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
title_sort	The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe
dc.creator.none.fl_str_mv	Wallace, Megan A. Coffman, Kelsey A. Gilbert, Clément Ravindran, Sanjana Albery, Gregory F. Abbott, Jessica Argyridou, Eliza Bellosta, Paola Betancourt, Andrea J. Colinet, Hervé Eric, Katarina Glaser-Schmitt, Amanda Grath, Sonja Jelic, Mihailo Kankare, Maaria Kozeretska, Iryna Loeschcke, Volker Montchamp-Moreau, Catherine Ometto, Lino Onder, Banu Sebnem Orengo Ferriz, Dorcas J. Parsch, John Pascual Berniola, Marta Patenkovic, Aleksandra Puerma Rodríguez, Eva María Ritchie, Michael G. Rota-Stabelli, Omar Schou, Mads Fristrup Serga, Svitlana Stamenkovic-Radak, Marina Tanaskovic, Marija Savic Veselinovic, Marija Vieira, Jorge Vieira, Cristina P. Kapun, Martin Flatt, Thomas González, Josefa Staubach, Fabian Obbard, Darren J.
author	Wallace, Megan A.
author_facet	Wallace, Megan A. Coffman, Kelsey A. Gilbert, Clément Ravindran, Sanjana Albery, Gregory F. Abbott, Jessica Argyridou, Eliza Bellosta, Paola Betancourt, Andrea J. Colinet, Hervé Eric, Katarina Glaser-Schmitt, Amanda Grath, Sonja Jelic, Mihailo Kankare, Maaria Kozeretska, Iryna Loeschcke, Volker Montchamp-Moreau, Catherine Ometto, Lino Onder, Banu Sebnem Orengo Ferriz, Dorcas J. Parsch, John Pascual Berniola, Marta Patenkovic, Aleksandra Puerma Rodríguez, Eva María Ritchie, Michael G. Rota-Stabelli, Omar Schou, Mads Fristrup Serga, Svitlana Stamenkovic-Radak, Marina Tanaskovic, Marija Savic Veselinovic, Marija Vieira, Jorge Vieira, Cristina P. Kapun, Martin Flatt, Thomas González, Josefa Staubach, Fabian Obbard, Darren J.
author_role	author
author2	Coffman, Kelsey A. Gilbert, Clément Ravindran, Sanjana Albery, Gregory F. Abbott, Jessica Argyridou, Eliza Bellosta, Paola Betancourt, Andrea J. Colinet, Hervé Eric, Katarina Glaser-Schmitt, Amanda Grath, Sonja Jelic, Mihailo Kankare, Maaria Kozeretska, Iryna Loeschcke, Volker Montchamp-Moreau, Catherine Ometto, Lino Onder, Banu Sebnem Orengo Ferriz, Dorcas J. Parsch, John Pascual Berniola, Marta Patenkovic, Aleksandra Puerma Rodríguez, Eva María Ritchie, Michael G. Rota-Stabelli, Omar Schou, Mads Fristrup Serga, Svitlana Stamenkovic-Radak, Marina Tanaskovic, Marija Savic Veselinovic, Marija Vieira, Jorge Vieira, Cristina P. Kapun, Martin Flatt, Thomas González, Josefa Staubach, Fabian Obbard, Darren J.
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Virus ADN Drosòfila DNA viruses Drosophila
topic	Virus ADN Drosòfila DNA viruses Drosophila
description	Drosophila melanogaster is an important model for antiviral immunity in arthropods, but very few DNA viruses have been described from the family Drosophilidae. This has limited our opportunity to use natural host-pathogen combinations in experimental studies, and may have biased our understanding of the Drosophila virome. Here we report fourteen DNA viruses detectable by metagenomic analysis of six and a half thousand pool-sequenced Drosophila, sampled from 47 European locations between 2014 and 2016. These include three new Nudiviruses, a new and divergent Entomopox virus, a virus related to Leptopilina boulardi filamentous virus, and a virus related to Musca domestica salivary gland hypertrophy virus. We also find an endogenous genomic copy of Galbut virus, a dsRNA Partitivirus, segregating at very low frequency. Remarkably, we show that Drosophila Vesanto virus, a small DNA virus previously described as a Bidnavirus, may be composed of up to 12 segments and represent a new lineage of segmented DNA viruses. Only two of the DNA viruses, Drosophila Kallithea nudivirus and Drosophila Vesanto virus are relatively common, being found in 2% or more of wild flies. The other viruses are rare, with many likely to be represented by a single infected fly. We find that virus prevalence in Europe reflects the prevalence seen in publicly-available datasets, with Drosophila Kallithea nudivirus and Drosophila Vesanto virus only being commonly detectable in public data from wild-caught flies and large population cages, and the other viruses being rare or absent. These analyses suggest that DNA viruses are generally at lower prevalence than RNA viruses in D. melanogaster, and may be less likely to persist in laboratory cultures. Our findings go some way to redress an earlier bias toward RNA virus studies in Drosophila, and lay the foundation needed to harness the power of Drosophila as a model system for the study of DNA viruses.
publishDate	2021
dc.date.none.fl_str_mv	2021 2023 2023 2023
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/197754
url	https://hdl.handle.net/2445/197754
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.1093/ve/veab031 Virus Evolution, 2021, vol. 7, num. 1, p. 1-23 https://doi.org/10.1093/ve/veab031
dc.rights.none.fl_str_mv	cc-by (c) Wallace, Megan A. et al., 2021 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by (c) Wallace, Megan A. et al., 2021 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	23 p. application/pdf
dc.publisher.none.fl_str_mv	Oxford University Press
publisher.none.fl_str_mv	Oxford University Press
dc.source.none.fl_str_mv	Articles publicats en revistes (Genètica, Microbiologia i Estadística) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe

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