Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples

The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their dete...

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Autores: Peñarrubia Lozano, Luis, Alcaraz Cazorla, Carles, Vaate, Abraham bij de, Sanz Ball-llosera, Núria, Pla Zanuy, Carles, Vidal i Fàbrega, Oriol, Viñas de Puig, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/14030
Acceso en línea:http://hdl.handle.net/10256/14030
Access Level:acceso abierto
Palabra clave:Genètica de poblacions
Population genetics
Musclos d’aigua dolça -- Genètica
Freshwater mussels -- Genetics
Animals invasors
Invasions biològiques
Introduced organisms
Biological invasions
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spelling Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samplesPeñarrubia Lozano, LuisAlcaraz Cazorla, CarlesVaate, Abraham bij deSanz Ball-llosera, NúriaPla Zanuy, CarlesVidal i Fàbrega, OriolViñas de Puig, JordiGenètica de poblacionsPopulation geneticsMusclos d’aigua dolça -- GenèticaFreshwater mussels -- GeneticsAnimals invasorsInvasions biològiquesIntroduced organismsBiological invasionsThe zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locationsWe are indebted to different colleagues for zebra mussel sample collections: Concha Durán (Ebro Hydrografic Confederation, Spain) for the logistic advice; Anna Terrats and Carolina Solà (Catalan Water Agency from the Government of Catalonia, Spain) for the filtered-water environmental samples. We would thank to Mariona Palacios for her collaboration in the analysis into her Final Master Project. This research was carried out within the objectives of the research project CGL200909407 of the Spanish Ministerio de Ciencia e Innovación (MICINN)Nature Publishing GroupMinisterio de Ciencia e Innovación (Espanya)2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/14030http://hdl.handle.net/10256/14030Scientific Reports, núm. 6, art. 39067Articles publicats (D-B)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep39067info:eu-repo/semantics/altIdentifier/issn/2045-2322MICINN/PN 2010-2013/CGL2009-09407Attribution 4.0 Spainhttp://creativecommons.org/licenses/by/4.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/140302026-05-29T05:05:01Z
dc.title.none.fl_str_mv Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
spellingShingle Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
Peñarrubia Lozano, Luis
Genètica de poblacions
Population genetics
Musclos d’aigua dolça -- Genètica
Freshwater mussels -- Genetics
Animals invasors
Invasions biològiques
Introduced organisms
Biological invasions
title_short Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_full Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_fullStr Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_full_unstemmed Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_sort Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
dc.creator.none.fl_str_mv Peñarrubia Lozano, Luis
Alcaraz Cazorla, Carles
Vaate, Abraham bij de
Sanz Ball-llosera, Núria
Pla Zanuy, Carles
Vidal i Fàbrega, Oriol
Viñas de Puig, Jordi
author Peñarrubia Lozano, Luis
author_facet Peñarrubia Lozano, Luis
Alcaraz Cazorla, Carles
Vaate, Abraham bij de
Sanz Ball-llosera, Núria
Pla Zanuy, Carles
Vidal i Fàbrega, Oriol
Viñas de Puig, Jordi
author_role author
author2 Alcaraz Cazorla, Carles
Vaate, Abraham bij de
Sanz Ball-llosera, Núria
Pla Zanuy, Carles
Vidal i Fàbrega, Oriol
Viñas de Puig, Jordi
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (Espanya)
dc.subject.none.fl_str_mv Genètica de poblacions
Population genetics
Musclos d’aigua dolça -- Genètica
Freshwater mussels -- Genetics
Animals invasors
Invasions biològiques
Introduced organisms
Biological invasions
topic Genètica de poblacions
Population genetics
Musclos d’aigua dolça -- Genètica
Freshwater mussels -- Genetics
Animals invasors
Invasions biològiques
Introduced organisms
Biological invasions
description The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/14030
http://hdl.handle.net/10256/14030
url http://hdl.handle.net/10256/14030
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep39067
info:eu-repo/semantics/altIdentifier/issn/2045-2322
MICINN/PN 2010-2013/CGL2009-09407
dc.rights.none.fl_str_mv Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific Reports, núm. 6, art. 39067
Articles publicats (D-B)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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