Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid

Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene sy...

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Autores: Farré Martinez, Gemma, Perez-Fons, Laura, Decourcelle , Mathilde, Breitenbach , Jürgen, Hem , Sonia, Zhu , Changfu, Capell Capell, Teresa, Christou , Paul, Fraser , Paul, Sandmann, Gerhard
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/468950
Acceso en línea:https://doi.org/10.1007/s11248-016-9943-7
https://hdl.handle.net/10459.1/468950
Access Level:acceso abierto
Palabra clave:Astaxanthin
Genetically engineered carotenoid biosynthesis
GM maize
Metabolomics
Transcriptomics
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spelling Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybridFarré Martinez, GemmaPerez-Fons, LauraDecourcelle , MathildeBreitenbach , JürgenHem , SoniaZhu , ChangfuCapell Capell, TeresaChristou , PaulFraser , PaulSandmann, GerhardAstaxanthinGenetically engineered carotenoid biosynthesisGM maizeMetabolomicsTranscriptomicsMaize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the controlling enzyme of carotenogenesis, was over-expressed for enhanced carotenoid production and lycopene ε-cyclase was knocked-down to direct more precursors into the β-branch of the extended ketocarotenoid pathway which ends with astaxanthin. This astaxanthin-accumulating transgenic line was crossed into a high oil- maize genotype in order to increase the storage capacity for lipophilic astaxanthin. The high oil astaxanthin hybrid was compared to its astaxanthin producing parent. We report an in depth metabolomic and proteomic analysis which revealed major up- or down- regulation of genes involved in primary metabolism. Specifically, amino acid biosynthesis and the citric acid cycle which compete with the synthesis or utilization of pyruvate and glyceraldehyde 3-phosphate, the precursors for carotenogenesis, were down-regulated. Nevertheless, principal component analysis demonstrated that this compositional change is within the range of the two wild type parents used to generate the high oil producing astaxanthin hybrid.Funding through the Plant KBBE project CaroMaize is gratefully acknowledged. Further support to PC was by the Ministerio de Economia y Competitividad, Spain (BIO2014-54441-P, BIO2011-22525) and a European Research Council Advanced Grant (BIOFORCE); PROGRAMA ESTATAL DE INVESTIGACIÓN CIENTÍFICA Y TÉCNICA DE EXCELENCIA, Spain (BIO2015-71703-REDT). PDF and LP are grateful for funding from the EU FP7 project DISCO grant number 613513. We thank Sys2Diag team (CNRS, France) for statistical analyses of proteomic data and particularly Nicolas Salvetat and Franck Molina.Springer2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://doi.org/10.1007/s11248-016-9943-7https://hdl.handle.net/10459.1/468950reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MINECO//BIO2014-54441-Pinfo:eu-repo/grantAgreement/MICINN//BIO2011-22525info:eu-repo/grantAgreement/MINECO//BIO2015-71703-REDTinfo:eu-repo/grantAgreement/EC/FP7/613513Versió postprint del document publicat a: https://doi.org/10.1007/s11248-016-9943-7Transgenic Research, 2016, vol.25, p. 477–489.cc-by-nc-nd, (c) Springer International Publishing Switzerland, 2016Attribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:repositori.udl.cat:10459.1/4689502026-06-24T12:42:17Z
dc.title.none.fl_str_mv Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
title Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
spellingShingle Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
Farré Martinez, Gemma
Astaxanthin
Genetically engineered carotenoid biosynthesis
GM maize
Metabolomics
Transcriptomics
title_short Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
title_full Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
title_fullStr Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
title_full_unstemmed Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
title_sort Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid
dc.creator.none.fl_str_mv Farré Martinez, Gemma
Perez-Fons, Laura
Decourcelle , Mathilde
Breitenbach , Jürgen
Hem , Sonia
Zhu , Changfu
Capell Capell, Teresa
Christou , Paul
Fraser , Paul
Sandmann, Gerhard
author Farré Martinez, Gemma
author_facet Farré Martinez, Gemma
Perez-Fons, Laura
Decourcelle , Mathilde
Breitenbach , Jürgen
Hem , Sonia
Zhu , Changfu
Capell Capell, Teresa
Christou , Paul
Fraser , Paul
Sandmann, Gerhard
author_role author
author2 Perez-Fons, Laura
Decourcelle , Mathilde
Breitenbach , Jürgen
Hem , Sonia
Zhu , Changfu
Capell Capell, Teresa
Christou , Paul
Fraser , Paul
Sandmann, Gerhard
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Astaxanthin
Genetically engineered carotenoid biosynthesis
GM maize
Metabolomics
Transcriptomics
topic Astaxanthin
Genetically engineered carotenoid biosynthesis
GM maize
Metabolomics
Transcriptomics
description Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the controlling enzyme of carotenogenesis, was over-expressed for enhanced carotenoid production and lycopene ε-cyclase was knocked-down to direct more precursors into the β-branch of the extended ketocarotenoid pathway which ends with astaxanthin. This astaxanthin-accumulating transgenic line was crossed into a high oil- maize genotype in order to increase the storage capacity for lipophilic astaxanthin. The high oil astaxanthin hybrid was compared to its astaxanthin producing parent. We report an in depth metabolomic and proteomic analysis which revealed major up- or down- regulation of genes involved in primary metabolism. Specifically, amino acid biosynthesis and the citric acid cycle which compete with the synthesis or utilization of pyruvate and glyceraldehyde 3-phosphate, the precursors for carotenogenesis, were down-regulated. Nevertheless, principal component analysis demonstrated that this compositional change is within the range of the two wild type parents used to generate the high oil producing astaxanthin hybrid.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1007/s11248-016-9943-7
https://hdl.handle.net/10459.1/468950
url https://doi.org/10.1007/s11248-016-9943-7
https://hdl.handle.net/10459.1/468950
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//BIO2014-54441-P
info:eu-repo/grantAgreement/MICINN//BIO2011-22525
info:eu-repo/grantAgreement/MINECO//BIO2015-71703-REDT
info:eu-repo/grantAgreement/EC/FP7/613513
Versió postprint del document publicat a: https://doi.org/10.1007/s11248-016-9943-7
Transgenic Research, 2016, vol.25, p. 477–489.
dc.rights.none.fl_str_mv cc-by-nc-nd, (c) Springer International Publishing Switzerland, 2016
Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv cc-by-nc-nd, (c) Springer International Publishing Switzerland, 2016
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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