Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy

Mesoporous silica nanoparticles (MSNPs) have many potential applications in biomedical fields. However, when MSNPs are exposed to plasma, protein adsorption leads to opsonization and decreases blood circulation time. A new multifunctional nanodevice based on polyethylenimine (PEI) coated core-shell...

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Authors: Sánchez Salcedo, Sandra, Vallet Regí, María Dulce Nombre, Allaf Shanin, Sophia, Zinck, Jeffrey I., Glackin, Carlotta A.
Format: article
Publication Date:2018
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/11948
Online Access:https://hdl.handle.net/20.500.14352/11948
Access Level:Open access
Keyword:546
615.46
Zwitterion
PEI core-shell MSNPs
Co-delivery
siRNA
Daunorubicin
Cancer therapy
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
id ES_2b991527e04d2bdcb769f7af4d1d2a05
oai_identifier_str oai:docta.ucm.es:20.500.14352/11948
network_acronym_str ES
network_name_str España
repository_id_str
spelling Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapySánchez Salcedo, SandraVallet Regí, María Dulce NombreAllaf Shanin, SophiaZinck, Jeffrey I.Glackin, Carlotta A.546615.46ZwitterionPEI core-shell MSNPsCo-deliverysiRNADaunorubicinCancer therapyMaterialesQuímica inorgánica (Química)3312 Tecnología de Materiales2303 Química InorgánicaMesoporous silica nanoparticles (MSNPs) have many potential applications in biomedical fields. However, when MSNPs are exposed to plasma, protein adsorption leads to opsonization and decreases blood circulation time. A new multifunctional nanodevice based on polyethylenimine (PEI) coated core-shell Fe⁠3O⁠4@SiO⁠2 MSNPs with a zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) surface was designed to minimize unspecific protein adhesion. Particle size measurements demonstrated an excellent non-fouling capacity in solutions containing Bovine Serum Albumin (BSA) and Fetal Bovine Serum (FBS) plasma proteins. The system was used in this study to co-deliver two different cargos: siRNA and daunorubicin. Anti-TWIST siRNA plays critical role in modulating knockdown of TWIST and sensitizing cells to chemotherapeutics such as daunorubicin for ovarian cancer therapy. The drug was released in response to externally controlled oscillating magnetic fields (OMF). siRNA (siGFP) silenced expression of green fluorescence protein (GFP) in Ovcar8 cancer cells, demonstrating the incorporation of core shell MSNPs into cells and siGFP delivery. The synergistic effect of the co-release of anti-TWIST-siRNA loaded in the PEI and daunorubicin loaded in NPs’ pores caused increased cytotoxicity in Ovcar8 of up to 50% from both zwitteronic and non-zwitteronic NPs. The system is the first example of silencing by anti-TWITS-siRNA/daunorubicin co-delivered using zwitterionic core-shell nanoparticles with low-fouling adsorption. This engineered multifunctional approach may provide therapeutic potential for the treatment of currently incurable ovarian cáncer.ElsevierUniversidad Complutense de Madrid20182018-01-0720182018-01-07journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/11948reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/119482026-06-02T12:44:21Z
dc.title.none.fl_str_mv Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
title Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
spellingShingle Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
Sánchez Salcedo, Sandra
546
615.46
Zwitterion
PEI core-shell MSNPs
Co-delivery
siRNA
Daunorubicin
Cancer therapy
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
title_short Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
title_full Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
title_fullStr Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
title_full_unstemmed Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
title_sort Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy
dc.creator.none.fl_str_mv Sánchez Salcedo, Sandra
Vallet Regí, María Dulce Nombre
Allaf Shanin, Sophia
Zinck, Jeffrey I.
Glackin, Carlotta A.
author Sánchez Salcedo, Sandra
author_facet Sánchez Salcedo, Sandra
Vallet Regí, María Dulce Nombre
Allaf Shanin, Sophia
Zinck, Jeffrey I.
Glackin, Carlotta A.
author_role author
author2 Vallet Regí, María Dulce Nombre
Allaf Shanin, Sophia
Zinck, Jeffrey I.
Glackin, Carlotta A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 546
615.46
Zwitterion
PEI core-shell MSNPs
Co-delivery
siRNA
Daunorubicin
Cancer therapy
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
topic 546
615.46
Zwitterion
PEI core-shell MSNPs
Co-delivery
siRNA
Daunorubicin
Cancer therapy
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
description Mesoporous silica nanoparticles (MSNPs) have many potential applications in biomedical fields. However, when MSNPs are exposed to plasma, protein adsorption leads to opsonization and decreases blood circulation time. A new multifunctional nanodevice based on polyethylenimine (PEI) coated core-shell Fe⁠3O⁠4@SiO⁠2 MSNPs with a zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) surface was designed to minimize unspecific protein adhesion. Particle size measurements demonstrated an excellent non-fouling capacity in solutions containing Bovine Serum Albumin (BSA) and Fetal Bovine Serum (FBS) plasma proteins. The system was used in this study to co-deliver two different cargos: siRNA and daunorubicin. Anti-TWIST siRNA plays critical role in modulating knockdown of TWIST and sensitizing cells to chemotherapeutics such as daunorubicin for ovarian cancer therapy. The drug was released in response to externally controlled oscillating magnetic fields (OMF). siRNA (siGFP) silenced expression of green fluorescence protein (GFP) in Ovcar8 cancer cells, demonstrating the incorporation of core shell MSNPs into cells and siGFP delivery. The synergistic effect of the co-release of anti-TWIST-siRNA loaded in the PEI and daunorubicin loaded in NPs’ pores caused increased cytotoxicity in Ovcar8 of up to 50% from both zwitteronic and non-zwitteronic NPs. The system is the first example of silencing by anti-TWITS-siRNA/daunorubicin co-delivered using zwitterionic core-shell nanoparticles with low-fouling adsorption. This engineered multifunctional approach may provide therapeutic potential for the treatment of currently incurable ovarian cáncer.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-07
2018
2018-01-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/11948
url https://hdl.handle.net/20.500.14352/11948
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405159029735424
score 15,300719

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