Bacterial communities associated with an island radiation of lichen-forming fungi

Evolutionary radiations are one of the most striking processes biologists have studied in islands. A radiation is often sparked by the appearance of ecological opportunity, which can originate in processes like trophic niche segregation or the evolution of key innovations. Another recently proposed...

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Detalles Bibliográficos
Autores: Blázquez, Miguel, Ortiz-Álvarez, Rüdiger, Gasulla, Francisco, Pérez-Vargas, Israel, Pérez-Ortega, Sergio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/371480
Acceso en línea:http://hdl.handle.net/10261/371480
Access Level:acceso abierto
Palabra clave:Acidobacteria
Actinobacteria
Alphaproteobacteria
Amplicon sequence variant
Article
Bacterium identification
Bacteroidetes
Biofilm
Bioinformatics
Caulobacteraceae
Community structure
Cyanobacterium
DNA extraction
DNA isolation
Firmicutes
Functional prediction
Fusobacteria
Gene amplification
Gene sequence
Genetic variability
Geographic distribution
High throughput sequencing
Island radiation
Lichen (organism)
Microbial community
Microbial diversity
Microbiome
Nonhuman
phylogenetic tree
phylogeny
phylosymbiosis
polymerase chain reaction
prediction
pyrosequencing
Ramalina delicata
Ramalina fortunatarum
Ramalina papyracea
Ramalina sabinosana
Ramalina sampaioana
Sequence alignment
Solar radiation
Symbiosist
Taxonomy
Bacterium
Ungus
Genetics
Microflora
Bacteria
Fungi
Lichens
Microbiota
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Descripción
Sumario:Evolutionary radiations are one of the most striking processes biologists have studied in islands. A radiation is often sparked by the appearance of ecological opportunity, which can originate in processes like trophic niche segregation or the evolution of key innovations. Another recently proposed mechanism is facilitation mediated by the bacterial communities associated with the radiating species. Here we explore the role of the bacterial communities in a radiation of lichen-forming fungi endemic to Macaronesia. Bacterial diversity was quantified by high throughput sequencing of the V1–V2 hyper-variable region of 172 specimens. We characterized the taxonomic and phylogenetic diversity of the bacterial communities associated with the different species, tested for compositional differences between these communities, carried out a functional prediction, explored the relative importance of different factors in bacterial community structure, searched for phylosymbiosis and tried to identify the origin of this pattern. The species of the radiation differed in the composition of their bacterial communities, which were mostly comprised of Alphaproteobacteria and Acidobacteriia, but not in the functionality of those communities. A phylosimbiotic pattern was detected, but it was probably caused by environmental filtering. These findings are congruent with the combined effect of secondary chemistry and mycobiont identity being the main driver of bacterial community structure. Altogether, our results suggest that the associated bacterial communities are not the radiation’s main driver. There is one possible exception, however, a species that has an abnormally diverse core microbiome and whose bacterial communities could be subject to a specific environmental filter at the functional level.