Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)

Na-Phatthalung, Pinanong|||0000-0002-8162-4645; Teles, Mariana|||0000-0001-5525-4049; Voravuthikunchai, S. P.; Tort Bardolet, Lluís|||0000-0002-3907-722X; Fierro Castro, Maria Camino|||0000-0002-5722-7471

Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)

Rhodomyrtus tomentosa is a medicinal plant that shows biological effects including immunomodulatory activity on human and other mammals but not in fish. In this study, we evaluated the in vitro immunomodulatory effects of R. tomentosa leaf extract and its active compound, rhodomyrtone, on the immune...

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Autores:	Na-Phatthalung, Pinanong\|\|\|0000-0002-8162-4645, Teles, Mariana\|\|\|0000-0001-5525-4049, Voravuthikunchai, S. P., Tort Bardolet, Lluís\|\|\|0000-0002-3907-722X, Fierro Castro, Maria Camino\|\|\|0000-0002-5722-7471
Tipo de recurso:	artículo
Fecha de publicación:	2018
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:186847
Acceso en línea:	https://ddd.uab.cat/record/186847 https://dx.doi.org/urn:doi:10.1007/s10695-017-0452-2
Access Level:	acceso abierto
Palabra clave:	Immunomodulatory effects Macrophages Rainbow trout Rhodomyrtone Rhodomyrtus tomentosa

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spelling	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)Na-Phatthalung, Pinanong\|\|\|0000-0002-8162-4645Teles, Mariana\|\|\|0000-0001-5525-4049Voravuthikunchai, S. P.Tort Bardolet, Lluís\|\|\|0000-0002-3907-722XFierro Castro, Maria Camino\|\|\|0000-0002-5722-7471Immunomodulatory effectsMacrophagesRainbow troutRhodomyrtoneRhodomyrtus tomentosaRhodomyrtus tomentosa is a medicinal plant that shows biological effects including immunomodulatory activity on human and other mammals but not in fish. In this study, we evaluated the in vitro immunomodulatory effects of R. tomentosa leaf extract and its active compound, rhodomyrtone, on the immune responses, using rainbow trout (Oncorhynchus mykiss) head kidney (HK) macrophages as a model. The tested immune functions included the expression of genes involved in innate immune and inflammatory responses and the production of reactive oxygen species (ROS). Gene expression was evaluated after exposure to 10 μg mL⁻¹ of R. tomentosa and 1 μg mL⁻¹ of rhodomyrtone for 4 and 24 h. R. tomentosa and rhodomyrtone induced changes in the expression of pro-inflammatory cytokines (il1β, il8, and tnfα), anti-inflammatory cytokines (il10 and tgfβ), inducible enzymes (inos, cox2, and arginase), and an antioxidant enzyme (gpx1). Co-exposure of R. tomentosa with LPS resulted in a prominent reduction in the expression of genes related to an inflammatory process (il1β, il8, tnfα, inos, saa, hepcidin, and gpx1), suggesting anti-inflammatory effects. Similarly, co-exposure of rhodomyrtone with LPS led to a downregulation of inflammation-related genes (il1β, inos, saa, and hepcidin). In addition, exposure to both natural plant products caused a reduction in cellular ROS levels by HK macrophages. The present results indicate that R. tomentosa and rhodomyrtone exerted immunostimulatory and anti-inflammatory effects on fish macrophages, thus opening up the possibility of using these natural products to further develop immunostimulants for health management in aquaculture. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/186847https://dx.doi.org/urn:doi:10.1007/s10695-017-0452-2reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2013-48835-C2open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1868472026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
title	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
spellingShingle	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss) Na-Phatthalung, Pinanong\|\|\|0000-0002-8162-4645 Immunomodulatory effects Macrophages Rainbow trout Rhodomyrtone Rhodomyrtus tomentosa
title_short	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
title_full	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
title_fullStr	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
title_full_unstemmed	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
title_sort	Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)
dc.creator.none.fl_str_mv	Na-Phatthalung, Pinanong\|\|\|0000-0002-8162-4645 Teles, Mariana\|\|\|0000-0001-5525-4049 Voravuthikunchai, S. P. Tort Bardolet, Lluís\|\|\|0000-0002-3907-722X Fierro Castro, Maria Camino\|\|\|0000-0002-5722-7471
author	Na-Phatthalung, Pinanong\|\|\|0000-0002-8162-4645
author_facet	Na-Phatthalung, Pinanong\|\|\|0000-0002-8162-4645 Teles, Mariana\|\|\|0000-0001-5525-4049 Voravuthikunchai, S. P. Tort Bardolet, Lluís\|\|\|0000-0002-3907-722X Fierro Castro, Maria Camino\|\|\|0000-0002-5722-7471
author_role	author
author2	Teles, Mariana\|\|\|0000-0001-5525-4049 Voravuthikunchai, S. P. Tort Bardolet, Lluís\|\|\|0000-0002-3907-722X Fierro Castro, Maria Camino\|\|\|0000-0002-5722-7471
author2_role	author author author author
dc.subject.none.fl_str_mv	Immunomodulatory effects Macrophages Rainbow trout Rhodomyrtone Rhodomyrtus tomentosa
topic	Immunomodulatory effects Macrophages Rainbow trout Rhodomyrtone Rhodomyrtus tomentosa
description	Rhodomyrtus tomentosa is a medicinal plant that shows biological effects including immunomodulatory activity on human and other mammals but not in fish. In this study, we evaluated the in vitro immunomodulatory effects of R. tomentosa leaf extract and its active compound, rhodomyrtone, on the immune responses, using rainbow trout (Oncorhynchus mykiss) head kidney (HK) macrophages as a model. The tested immune functions included the expression of genes involved in innate immune and inflammatory responses and the production of reactive oxygen species (ROS). Gene expression was evaluated after exposure to 10 μg mL⁻¹ of R. tomentosa and 1 μg mL⁻¹ of rhodomyrtone for 4 and 24 h. R. tomentosa and rhodomyrtone induced changes in the expression of pro-inflammatory cytokines (il1β, il8, and tnfα), anti-inflammatory cytokines (il10 and tgfβ), inducible enzymes (inos, cox2, and arginase), and an antioxidant enzyme (gpx1). Co-exposure of R. tomentosa with LPS resulted in a prominent reduction in the expression of genes related to an inflammatory process (il1β, il8, tnfα, inos, saa, hepcidin, and gpx1), suggesting anti-inflammatory effects. Similarly, co-exposure of rhodomyrtone with LPS led to a downregulation of inflammation-related genes (il1β, inos, saa, and hepcidin). In addition, exposure to both natural plant products caused a reduction in cellular ROS levels by HK macrophages. The present results indicate that R. tomentosa and rhodomyrtone exerted immunostimulatory and anti-inflammatory effects on fish macrophages, thus opening up the possibility of using these natural products to further develop immunostimulants for health management in aquaculture.
publishDate	2018
dc.date.none.fl_str_mv	2 2018-01-01 2018 2018-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/186847 https://dx.doi.org/urn:doi:10.1007/s10695-017-0452-2
url	https://ddd.uab.cat/record/186847 https://dx.doi.org/urn:doi:10.1007/s10695-017-0452-2
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2013-48835-C2
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
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Immunomodulatory effects of Rhodomyrtus tomentosa leaf extract and its derivative compound, rhodomyrtone, on head kidney macrophages of rainbow trout (Oncorhynchus mykiss)

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