Hybridization and melting behavior of Peptide Nucleic Acid (PNA) oligonucleotide chimeras conjugated to gold nanoparticles
Peptide nucleic acids (PNA) and PNA-DNA chimeras carrying thiol groups were used for surface functionalization of Au nanoparticles. Conjugation of PNA to citrate-stabilized Au nanoparticles destabilized the nanoparticles causing them to precipitate. Addition of a tail of glutamic acid to the PNA pre...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2004 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/124863 |
| Acceso en línea: | http://hdl.handle.net/10261/124863 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA Gold Nanostructured materials Organic polymers Nucleic acids Glutamic acid Olegonucleotides |
| Sumario: | Peptide nucleic acids (PNA) and PNA-DNA chimeras carrying thiol groups were used for surface functionalization of Au nanoparticles. Conjugation of PNA to citrate-stabilized Au nanoparticles destabilized the nanoparticles causing them to precipitate. Addition of a tail of glutamic acid to the PNA prevented destabilization of the nanoparticles but resulted in loss of interaction with complementary sequences. Importantly, PNA-DNA chimeras gave stable conjugates with Au nanoparticles. The hybridization and melting properties of complexes formed from chimera-nanoparticle conjugates and oligonucleotide-nanoparticle conjugates are described for the first time. Similar to oligonucleotide- nanoparticle conjugates, conjugates with PNA-DNA chimeras gave sharper and more-defined melting profiles than those obtained with unmodified oligonucleotides. In addition, mismatch discrimination was found to be more efficient than with unmodified oligonucleotides. |
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