Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales

[EN]Osteoarthritis (OA) is highly prevalent disease worldwide, curses with pain and functional disability characterized by synovium inflammation as a result of the progressive cartilage destruction. Macrophages seem to have a pivotal role at the onset and development of this disease involved in the...

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Detalles Bibliográficos
Autor: Martínez Borrajo, Rebeca
Tipo de recurso: tesis de maestría
Fecha de publicación:2019
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:español
OAI Identifier:oai:minerva.usc.gal:10347/20380
Acceso en línea:http://hdl.handle.net/10347/20380
Access Level:acceso abierto
Palabra clave:Materias::Investigación::32 Ciencias médicas::3208 Farmacodinámica
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spelling Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinovialesMartínez Borrajo, RebecaMaterias::Investigación::32 Ciencias médicas::3208 Farmacodinámica[EN]Osteoarthritis (OA) is highly prevalent disease worldwide, curses with pain and functional disability characterized by synovium inflammation as a result of the progressive cartilage destruction. Macrophages seem to have a pivotal role at the onset and development of this disease involved in the inflammatory process. When a cartilage lesion occurs, macrophages release pro-inflammatory mediators leading to cartilage destruction. Current OA therapy includes intraarticular corticosteroids and hyaluronic acid administration aiming at improving the pathology symptomatology, but without improvement in the cartilage regeneration due to, partially, the rapid clearance on the joint cavity. The aim of this work is to target synovial macrophages involved in OA through the intraarticular administration of nanoparticulated systems. To this end, nanostructured lipid carriers (NLCs) loaded with an anti-inflammatory drug were developed using artificial neural networks (ANN), the cellular response of those systems was analyzed in human macrophages and chondrocytes from patients with OA. The experimental results showed the developed systems had adequate characteristics of size and zeta potential with capacity to load the antiinflamatory drug β-Lapachone. The use of neural networks has allowed to choose the formulation composition able to fulfill the desired characteristics for intra-articular administration. The optimized formulation showed similar characteristics to neural networks prediction and is stable for at least two weeks. In addition, functionalization of optimized NLCs with mannose increases macrophages internalizationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía FarmacéuticaDíaz Rodríguez, PatriciaLandín Pérez, Mariana20192019-01-0120192019-01-01master thesishttp://purl.org/coar/resource_type/c_bdccinfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10347/20380reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Españolspaopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/203802026-06-15T12:47:27Z
dc.title.none.fl_str_mv Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
title Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
spellingShingle Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
Martínez Borrajo, Rebeca
Materias::Investigación::32 Ciencias médicas::3208 Farmacodinámica
title_short Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
title_full Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
title_fullStr Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
title_full_unstemmed Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
title_sort Sistemas nanoparticulados de administración intraarticular para vectorización de macrófagos sinoviales
dc.creator.none.fl_str_mv Martínez Borrajo, Rebeca
author Martínez Borrajo, Rebeca
author_facet Martínez Borrajo, Rebeca
author_role author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
Díaz Rodríguez, Patricia
Landín Pérez, Mariana
dc.subject.none.fl_str_mv Materias::Investigación::32 Ciencias médicas::3208 Farmacodinámica
topic Materias::Investigación::32 Ciencias médicas::3208 Farmacodinámica
description [EN]Osteoarthritis (OA) is highly prevalent disease worldwide, curses with pain and functional disability characterized by synovium inflammation as a result of the progressive cartilage destruction. Macrophages seem to have a pivotal role at the onset and development of this disease involved in the inflammatory process. When a cartilage lesion occurs, macrophages release pro-inflammatory mediators leading to cartilage destruction. Current OA therapy includes intraarticular corticosteroids and hyaluronic acid administration aiming at improving the pathology symptomatology, but without improvement in the cartilage regeneration due to, partially, the rapid clearance on the joint cavity. The aim of this work is to target synovial macrophages involved in OA through the intraarticular administration of nanoparticulated systems. To this end, nanostructured lipid carriers (NLCs) loaded with an anti-inflammatory drug were developed using artificial neural networks (ANN), the cellular response of those systems was analyzed in human macrophages and chondrocytes from patients with OA. The experimental results showed the developed systems had adequate characteristics of size and zeta potential with capacity to load the antiinflamatory drug β-Lapachone. The use of neural networks has allowed to choose the formulation composition able to fulfill the desired characteristics for intra-articular administration. The optimized formulation showed similar characteristics to neural networks prediction and is stable for at least two weeks. In addition, functionalization of optimized NLCs with mannose increases macrophages internalization
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv master thesis
http://purl.org/coar/resource_type/c_bdcc
dc.type.openaire.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/20380
url http://hdl.handle.net/10347/20380
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
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