Engineering and development of model lipid membranes mimicking the HeLa cell membrane

Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model memb...

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Autores: Botet Carreras, Adrià, Montero Barrientos, Ma. Teresa, Sot, Jesus, Domènech Cabrera, Òscar, Borrell Hernández, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/184788
Acceso en línea:https://hdl.handle.net/2445/184788
Access Level:acceso abierto
Palabra clave:Lípids
Proteïnes de membrana
Nanotecnologia
Lipids
Membrane proteins
Nanotechnology
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spelling Engineering and development of model lipid membranes mimicking the HeLa cell membraneBotet Carreras, AdriàMontero Barrientos, Ma. TeresaSot, JesusDomènech Cabrera, ÒscarBorrell Hernández, JordiLípidsProteïnes de membranaNanotecnologiaLipidsMembrane proteinsNanotechnologyCells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug deliveryElsevier B.V.2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/184788Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.colsurfa.2021.127663Colloids and Surfaces A-Physicochemical and Engineering Aspects, 2021, vol. 630, p. 127663https://doi.org/10.1016/j.colsurfa.2021.127663cc-by-nc-nd (c) Adrià Botet-Carreras, et al., 2021http://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1847882026-05-27T06:46:51Z
dc.title.none.fl_str_mv Engineering and development of model lipid membranes mimicking the HeLa cell membrane
title Engineering and development of model lipid membranes mimicking the HeLa cell membrane
spellingShingle Engineering and development of model lipid membranes mimicking the HeLa cell membrane
Botet Carreras, Adrià
Lípids
Proteïnes de membrana
Nanotecnologia
Lipids
Membrane proteins
Nanotechnology
title_short Engineering and development of model lipid membranes mimicking the HeLa cell membrane
title_full Engineering and development of model lipid membranes mimicking the HeLa cell membrane
title_fullStr Engineering and development of model lipid membranes mimicking the HeLa cell membrane
title_full_unstemmed Engineering and development of model lipid membranes mimicking the HeLa cell membrane
title_sort Engineering and development of model lipid membranes mimicking the HeLa cell membrane
dc.creator.none.fl_str_mv Botet Carreras, Adrià
Montero Barrientos, Ma. Teresa
Sot, Jesus
Domènech Cabrera, Òscar
Borrell Hernández, Jordi
author Botet Carreras, Adrià
author_facet Botet Carreras, Adrià
Montero Barrientos, Ma. Teresa
Sot, Jesus
Domènech Cabrera, Òscar
Borrell Hernández, Jordi
author_role author
author2 Montero Barrientos, Ma. Teresa
Sot, Jesus
Domènech Cabrera, Òscar
Borrell Hernández, Jordi
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lípids
Proteïnes de membrana
Nanotecnologia
Lipids
Membrane proteins
Nanotechnology
topic Lípids
Proteïnes de membrana
Nanotecnologia
Lipids
Membrane proteins
Nanotechnology
description Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, are continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of the processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported lipid bilayers (SLBs), mimicking the lipid composition of the HeLa cell plasma membrane. We characterized the model using atomic force microscopy (AFM) and fluorescence. We found that liposomes formed with four lipid components mimicking the HeLa cell bilayer show a liquid ordered fluid nature between 13 °C and 34 °C and yield featureless SLBs onto mica. We evaluated the fusion between the model and liposomes positively charged with and without cholesterol by AFM-based force spectroscopy and fluorescence techniques, such as Förster resonance energy transfer, fluorescence lifetime decay and fluorescence anisotropy. The results indicated a primary electrostatic interaction between the HeLa bilayer model and the liposomes. It was also confirmed the well-known fact that cholesterol enhances the fusion process with the engineered HeLa bilayer. All results support the usefulness of the engineered model in the rationale design of liposomes for drug delivery
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184788
url https://hdl.handle.net/2445/184788
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.colsurfa.2021.127663
Colloids and Surfaces A-Physicochemical and Engineering Aspects, 2021, vol. 630, p. 127663
https://doi.org/10.1016/j.colsurfa.2021.127663
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Adrià Botet-Carreras, et al., 2021
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Adrià Botet-Carreras, et al., 2021
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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