Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds

In response to sustainability concerns related to the origin of raw materials, aquaculture has emphasized the use of additives and enhancers to improve the intestinal absorption of proteins and amino acids. However, the tools available to have a good proxy for potential intestinal absorption and to...

ver descrição completa

Detalhes bibliográficos
Autores: Robles-Arozarena, Rocío, Martos-Sitcha, Juan Antonio, Bermúdez, Laura, Nuez-Ortín, Waldo, Fuentes, Juan
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/378412
Acesso em linha:http://hdl.handle.net/10261/378412
https://api.elsevier.com/content/abstract/scopus_id/85210546141
Access Level:acceso abierto
Palavra-chave:Electrophysiology
Fishmeal
Intestinal transport
Lysophospholipids
Sea bream
Somatic indices
Ussing chamber
id ES_2b2f4cba232b04a749d43100ad2a0b59
oai_identifier_str oai:digital.csic.es:10261/378412
network_acronym_str ES
network_name_str España
repository_id_str
spelling Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeedsRobles-Arozarena, RocíoMartos-Sitcha, Juan AntonioBermúdez, LauraNuez-Ortín, WaldoFuentes, JuanElectrophysiologyFishmealIntestinal transportLysophospholipidsSea breamSomatic indicesUssing chamberIn response to sustainability concerns related to the origin of raw materials, aquaculture has emphasized the use of additives and enhancers to improve the intestinal absorption of proteins and amino acids. However, the tools available to have a good proxy for potential intestinal absorption and to expose positive or negative effects are scarce. The present study had a two-fold objective: we first aimed to characterize electrogenic essential amino acid transport in the intestine of the gilthead seabream ex-vivo. We also aimed to understand if dietary lysophospholipids (LPLs) had positive or detrimental effects when incorporated into commercial diets in gilthead seabream (Sparus aurata) juveniles. The ex-vivo characterization in Ussing chambers showed a region-dependent electrogenic amino acid transport in the sea bream intestine, being maxima in the mid intestine and with a strong luminal sodium- and pH-dependence. In vivo, the animals were divided into two groups: one was fed a control diet (10 % fishmeal inclusion), whereas the other group was fed with the control diet supplemented with 0.1 % LPL in coated form. The LPL-inclusion was without effects on fish growth in a short-term feeding trial, although there was a significant improvement of hepatosomatic and viscerosomatic indices. Interestingly, the intestine of fish fed the aquafeed with a 0.1 % LPL supplementation increased paracellular permeability as measured in the anterior intestine and enhanced electrogenic amino acid transport in the mid intestine. Our results strongly suggest that the full potential of LPL-inclusion benefits could be explored in sustainable diets, nearly devoid of fishmeal and/or in fish subjected to environmental stress.his work has been partially co-financed by the European Union under the 2014–2020 ERDF Operational Programme and by the Department of Economic Transformation, Industry, Knowledge, and Universities of the Regional Government of Andalusia (Project reference: FEDER-UCA18–107182) and Project IRISer (PID2023–146411OB-C21 and PID2023–146411OB-C22), funded by The Spanish Ministry of Sciences, Innovation and Universities.Peer reviewedElsevier BVEuropean CommissionUniversidad de CádizJunta de AndalucíaAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Martos-Sitcha, Juan Antonio [0000-0002-0151-7250]Fuentes, Juan [0000-0003-0430-8734]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/378412https://api.elsevier.com/content/abstract/scopus_id/85210546141reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146411OB-C21info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146411OB-C22https://doi.org/10.1016/j.aqrep.2024.102539Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3784122026-05-22T06:33:51Z
dc.title.none.fl_str_mv Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
title Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
spellingShingle Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
Robles-Arozarena, Rocío
Electrophysiology
Fishmeal
Intestinal transport
Lysophospholipids
Sea bream
Somatic indices
Ussing chamber
title_short Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
title_full Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
title_fullStr Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
title_full_unstemmed Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
title_sort Electrogenic amino acid transport in the intestine of sea bream (Sparus aurata): From functional characterization to physiological effects of Lysophospholipids in aquafeeds
dc.creator.none.fl_str_mv Robles-Arozarena, Rocío
Martos-Sitcha, Juan Antonio
Bermúdez, Laura
Nuez-Ortín, Waldo
Fuentes, Juan
author Robles-Arozarena, Rocío
author_facet Robles-Arozarena, Rocío
Martos-Sitcha, Juan Antonio
Bermúdez, Laura
Nuez-Ortín, Waldo
Fuentes, Juan
author_role author
author2 Martos-Sitcha, Juan Antonio
Bermúdez, Laura
Nuez-Ortín, Waldo
Fuentes, Juan
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Universidad de Cádiz
Junta de Andalucía
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Martos-Sitcha, Juan Antonio [0000-0002-0151-7250]
Fuentes, Juan [0000-0003-0430-8734]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Electrophysiology
Fishmeal
Intestinal transport
Lysophospholipids
Sea bream
Somatic indices
Ussing chamber
topic Electrophysiology
Fishmeal
Intestinal transport
Lysophospholipids
Sea bream
Somatic indices
Ussing chamber
description In response to sustainability concerns related to the origin of raw materials, aquaculture has emphasized the use of additives and enhancers to improve the intestinal absorption of proteins and amino acids. However, the tools available to have a good proxy for potential intestinal absorption and to expose positive or negative effects are scarce. The present study had a two-fold objective: we first aimed to characterize electrogenic essential amino acid transport in the intestine of the gilthead seabream ex-vivo. We also aimed to understand if dietary lysophospholipids (LPLs) had positive or detrimental effects when incorporated into commercial diets in gilthead seabream (Sparus aurata) juveniles. The ex-vivo characterization in Ussing chambers showed a region-dependent electrogenic amino acid transport in the sea bream intestine, being maxima in the mid intestine and with a strong luminal sodium- and pH-dependence. In vivo, the animals were divided into two groups: one was fed a control diet (10 % fishmeal inclusion), whereas the other group was fed with the control diet supplemented with 0.1 % LPL in coated form. The LPL-inclusion was without effects on fish growth in a short-term feeding trial, although there was a significant improvement of hepatosomatic and viscerosomatic indices. Interestingly, the intestine of fish fed the aquafeed with a 0.1 % LPL supplementation increased paracellular permeability as measured in the anterior intestine and enhanced electrogenic amino acid transport in the mid intestine. Our results strongly suggest that the full potential of LPL-inclusion benefits could be explored in sustainable diets, nearly devoid of fishmeal and/or in fish subjected to environmental stress.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/378412
https://api.elsevier.com/content/abstract/scopus_id/85210546141
url http://hdl.handle.net/10261/378412
https://api.elsevier.com/content/abstract/scopus_id/85210546141
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146411OB-C21
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-146411OB-C22
https://doi.org/10.1016/j.aqrep.2024.102539
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405128406073344
score 15,811543

Registos relacionados