Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils

Advances in molecular techniques have recently uncovered microbial communities associated with plastic debris. The term ‘plastisphere’, was originally used for microbial communities on marine plastic. In terrestrial systems, this term has been extended to the plastic-soil interface, encompassing mic...

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Autores: Macan, Giovana P. F., Anguita-Maeso, Manuel, Olivares-García, Concepción, Le, Quynh Nhu Phan, Halsall, Crispin, Landa, Blanca B.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/399173
Acceso en línea:http://hdl.handle.net/10261/399173
https://api.elsevier.com/content/abstract/scopus_id/85216710058
Access Level:acceso abierto
Palabra clave:Soil health
Agricultural fields
Core bacteria
Environmental pollution
Microbial diversity
Plastisphere
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spelling Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soilsMacan, Giovana P. F.Anguita-Maeso, ManuelOlivares-García, ConcepciónLe, Quynh Nhu PhanHalsall, CrispinLanda, Blanca B.Soil healthAgricultural fieldsCore bacteriaEnvironmental pollutionMicrobial diversityPlastisphereAdvances in molecular techniques have recently uncovered microbial communities associated with plastic debris. The term ‘plastisphere’, was originally used for microbial communities on marine plastic. In terrestrial systems, this term has been extended to the plastic-soil interface, encompassing microbes from the surrounding soil. Although some studies have revealed differences in microbial composition and diversity between plastisphere and bulk soil, high-resolution spatial analyses of microbial communities on the immediate plastic surface (plasticplane) and in the attached soil (plastisphere-soil), are still lacking. In this study, a methodology was developed to disentangle the bacterial populations associated with the plastisphere-soil of weathered plastic mulch from agricultural fields from those on the plasticplane by using culture-based and High-Throughput sequencing approaches. A significantly higher number of colony-forming units were cultured from the plastisphere-soil compared to the plasticplane. Main genera isolated from the plasticplane by culturing included Arthrobacter, Pseudarthrobacter, Priestia, Massilia, Microbacterium, Bacillus, and Kocuria genera, some of which are known plastic-degraders. High-throughput sequencing analysis revealed higher bacterial richness in plastisphere-soil, while beta diversity showed main significant differences among field plots. Core taxa significantly associated to the plasticplane included Bacillus, Sphingomonas, Nocardioides, and Solirubrobacter. This study provides a pioneering description of a methodology to differentially analyze microbial communities at different soil-plastic interfaces, particularly on a small spatial scale using samples from plastic mulch residues in agricultural soils. This methodology may lay a foundation for future research to isolate and identify microbial plastic degraders, contributing to efforts against mitigating plastic pollution.We would like to acknowledge the financial support from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 955334 SOPLAS and the Qualifica Project QUAL21-023IAS, Consejeria de Universidad, Investigación y Inovación-Junta de Andalucía.Peer reviewedElsevierEuropean CommissionJunta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/399173https://api.elsevier.com/content/abstract/scopus_id/85216710058reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/955334The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.apsoil.2025.105900https://doi.org/10.1016/j.apsoil.2025.105900Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3991732026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
title Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
spellingShingle Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
Macan, Giovana P. F.
Soil health
Agricultural fields
Core bacteria
Environmental pollution
Microbial diversity
Plastisphere
title_short Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
title_full Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
title_fullStr Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
title_full_unstemmed Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
title_sort Unravelling the plastisphere-soil and plasticplane microbiome of plastic mulch residues in agricultural soils
dc.creator.none.fl_str_mv Macan, Giovana P. F.
Anguita-Maeso, Manuel
Olivares-García, Concepción
Le, Quynh Nhu Phan
Halsall, Crispin
Landa, Blanca B.
author Macan, Giovana P. F.
author_facet Macan, Giovana P. F.
Anguita-Maeso, Manuel
Olivares-García, Concepción
Le, Quynh Nhu Phan
Halsall, Crispin
Landa, Blanca B.
author_role author
author2 Anguita-Maeso, Manuel
Olivares-García, Concepción
Le, Quynh Nhu Phan
Halsall, Crispin
Landa, Blanca B.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Soil health
Agricultural fields
Core bacteria
Environmental pollution
Microbial diversity
Plastisphere
topic Soil health
Agricultural fields
Core bacteria
Environmental pollution
Microbial diversity
Plastisphere
description Advances in molecular techniques have recently uncovered microbial communities associated with plastic debris. The term ‘plastisphere’, was originally used for microbial communities on marine plastic. In terrestrial systems, this term has been extended to the plastic-soil interface, encompassing microbes from the surrounding soil. Although some studies have revealed differences in microbial composition and diversity between plastisphere and bulk soil, high-resolution spatial analyses of microbial communities on the immediate plastic surface (plasticplane) and in the attached soil (plastisphere-soil), are still lacking. In this study, a methodology was developed to disentangle the bacterial populations associated with the plastisphere-soil of weathered plastic mulch from agricultural fields from those on the plasticplane by using culture-based and High-Throughput sequencing approaches. A significantly higher number of colony-forming units were cultured from the plastisphere-soil compared to the plasticplane. Main genera isolated from the plasticplane by culturing included Arthrobacter, Pseudarthrobacter, Priestia, Massilia, Microbacterium, Bacillus, and Kocuria genera, some of which are known plastic-degraders. High-throughput sequencing analysis revealed higher bacterial richness in plastisphere-soil, while beta diversity showed main significant differences among field plots. Core taxa significantly associated to the plasticplane included Bacillus, Sphingomonas, Nocardioides, and Solirubrobacter. This study provides a pioneering description of a methodology to differentially analyze microbial communities at different soil-plastic interfaces, particularly on a small spatial scale using samples from plastic mulch residues in agricultural soils. This methodology may lay a foundation for future research to isolate and identify microbial plastic degraders, contributing to efforts against mitigating plastic pollution.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/399173
https://api.elsevier.com/content/abstract/scopus_id/85216710058
url http://hdl.handle.net/10261/399173
https://api.elsevier.com/content/abstract/scopus_id/85216710058
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/955334
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.apsoil.2025.105900
https://doi.org/10.1016/j.apsoil.2025.105900
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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