Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence

The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key...

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Detalhes bibliográficos
Autores: Cicuéndez Maroto, Mónica, Casarrubios Molina, Laura, Feito Castellano, María José, Madarieta, Iratxe, Garcií Urkia, Nerea, Murua, Olatz, Olalde. Beatriz, Nerea, Briz, Díez Orejas, Rosalía María, Portolés Pérez, María Teresa
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/8217
Acesso em linha:https://hdl.handle.net/20.500.14352/8217
Access Level:Acceso aberto
Palavra-chave:577.1
577.2
579
Extracellular matrix
Decellularization
Macrophage
Immunocompetence
Phagocytosis
Biología molecular (Química)
Bioquímica (Química)
Microbiología (Farmacia)
3302.03 Microbiología Industrial
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oai_identifier_str oai:docta.ucm.es:20.500.14352/8217
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and ImmunocompetenceCicuéndez Maroto, MónicaCasarrubios Molina, LauraFeito Castellano, María JoséMadarieta, IratxeGarcií Urkia, NereaMurua, OlatzOlalde. BeatrizNerea, BrizDíez Orejas, Rosalía MaríaPortolés Pérez, María Teresa577.1577.2579Extracellular matrixDecellularizationMacrophageImmunocompetencePhagocytosisBiología molecular (Química)Bioquímica (Química)Microbiología (Farmacia)3302.03 Microbiología IndustrialThe decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key role in the inflammation/healing processes due to their high functional plasticity between M1 and M2 phenotypes, the evaluation of their response to decellularized ECM is mandatory. It is also necessary to analyze the immunocompetence of macrophages after contact with decellularized ECM materials to assess their functional role in a possible infection scenario. In this work, we studied the effect of four decellularized adipose matrices (DAMs) obtained from human and porcine AT by enzymatic or chemical methods on macrophage phenotypes and fungal phagocytosis. First, a thorough biochemical characterization of these biomaterials by quantification of remnant DNA, lipids, and proteins was performed, thus indicating the efficiency and reliability of both methods. The proteomic analysis evidenced that some proteins are differentially preserved depending on both the AT origin and the decellularization method employed. After exposure to the four DAMs, specific markers of M1 proinflammatory and M2 anti-inflammatory macrophages were analyzed. Porcine DAMs favor the M2 phenotype, independently of the decellularization method employed. Finally, a sensitive fungal phagocytosis assay allowed us to relate the macrophage phagocytosis capability with specific proteins differentially preserved in certain DAMs. The results obtained in this study highlight the close relationship between the ECM biochemical composition and the macrophage’s functional role.MDPIUniversidad Complutense de Madrid20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/8217reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/82172026-06-02T12:44:21Z
dc.title.none.fl_str_mv Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
spellingShingle Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
Cicuéndez Maroto, Mónica
577.1
577.2
579
Extracellular matrix
Decellularization
Macrophage
Immunocompetence
Phagocytosis
Biología molecular (Química)
Bioquímica (Química)
Microbiología (Farmacia)
3302.03 Microbiología Industrial
title_short Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_full Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_fullStr Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_full_unstemmed Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_sort Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
dc.creator.none.fl_str_mv Cicuéndez Maroto, Mónica
Casarrubios Molina, Laura
Feito Castellano, María José
Madarieta, Iratxe
Garcií Urkia, Nerea
Murua, Olatz
Olalde. Beatriz
Nerea, Briz
Díez Orejas, Rosalía María
Portolés Pérez, María Teresa
author Cicuéndez Maroto, Mónica
author_facet Cicuéndez Maroto, Mónica
Casarrubios Molina, Laura
Feito Castellano, María José
Madarieta, Iratxe
Garcií Urkia, Nerea
Murua, Olatz
Olalde. Beatriz
Nerea, Briz
Díez Orejas, Rosalía María
Portolés Pérez, María Teresa
author_role author
author2 Casarrubios Molina, Laura
Feito Castellano, María José
Madarieta, Iratxe
Garcií Urkia, Nerea
Murua, Olatz
Olalde. Beatriz
Nerea, Briz
Díez Orejas, Rosalía María
Portolés Pérez, María Teresa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
577.2
579
Extracellular matrix
Decellularization
Macrophage
Immunocompetence
Phagocytosis
Biología molecular (Química)
Bioquímica (Química)
Microbiología (Farmacia)
3302.03 Microbiología Industrial
topic 577.1
577.2
579
Extracellular matrix
Decellularization
Macrophage
Immunocompetence
Phagocytosis
Biología molecular (Química)
Bioquímica (Química)
Microbiología (Farmacia)
3302.03 Microbiología Industrial
description The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key role in the inflammation/healing processes due to their high functional plasticity between M1 and M2 phenotypes, the evaluation of their response to decellularized ECM is mandatory. It is also necessary to analyze the immunocompetence of macrophages after contact with decellularized ECM materials to assess their functional role in a possible infection scenario. In this work, we studied the effect of four decellularized adipose matrices (DAMs) obtained from human and porcine AT by enzymatic or chemical methods on macrophage phenotypes and fungal phagocytosis. First, a thorough biochemical characterization of these biomaterials by quantification of remnant DNA, lipids, and proteins was performed, thus indicating the efficiency and reliability of both methods. The proteomic analysis evidenced that some proteins are differentially preserved depending on both the AT origin and the decellularization method employed. After exposure to the four DAMs, specific markers of M1 proinflammatory and M2 anti-inflammatory macrophages were analyzed. Porcine DAMs favor the M2 phenotype, independently of the decellularization method employed. Finally, a sensitive fungal phagocytosis assay allowed us to relate the macrophage phagocytosis capability with specific proteins differentially preserved in certain DAMs. The results obtained in this study highlight the close relationship between the ECM biochemical composition and the macrophage’s functional role.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/8217
url https://hdl.handle.net/20.500.14352/8217
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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