A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue

The tumor microenvironment comprises different cell subsets including myeloid-derived suppressor cells, which exert intratumoral immunosuppression and favor cancer progression. Isolating tumor-infiltrating myeloid-derived suppressor cells (tMDSCs) from human tumor samples remains a challenge. Curren...

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Autores: Piqueras-Nebot, M, Benet, M, Estors, M, Cremades, A, Juan-Vidal, O, Carretero, J, Galbis-Caravajal, JM, Lahoz, A
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p18848
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/18848
Access Level:acceso abierto
Palabra clave:Myeloid-derived suppressor cells
Human
Tumor-infiltrating
Isolation
Lung cancer
Immunosuppression
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spelling A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissuePiqueras-Nebot, MBenet, MEstors, MCremades, AJuan-Vidal, OCarretero, JGalbis-Caravajal, JMLahoz, AMyeloid-derived suppressor cellsHumanTumor-infiltratingIsolationLung cancerImmunosuppressionThe tumor microenvironment comprises different cell subsets including myeloid-derived suppressor cells, which exert intratumoral immunosuppression and favor cancer progression. Isolating tumor-infiltrating myeloid-derived suppressor cells (tMDSCs) from human tumor samples remains a challenge. Current methods such as magnetic bead sorting (MACS) or flow cytometry sorting (FACS) present some drawbacks in terms of purity and viability. Here, we have setup an innovative workflow that combines RosetteSep technology and MACS for isolation of tMDSCs from lung cancer biopsies. To evaluate our Rosette-MACS approach, we compared its performance with MACS and FACS. The isolated cells were characterized by flow cytometry, gene expression analysis and proliferation assays for comparison purposes. The results showed that the Rosette-MACS protocol had the highest yield and purity of tMDSCs (79.64% vs 13.30% with FACS and 0.39% with MACS). Furthermore, the functionality of the isolated tMDSCs was tested not only by upregulation of immunosuppressive genes (e.g. ARG1, IDO1, or PD-L1), but also by their capacity to inhibit CD8+ T cells proliferation. The combined use of RosetteSep and MACS provides an improved approach for the isolation of functional tMDSCs, which delineates a suitable experimental framework to selectively study the molecular mechanisms underpinning tMDSCs-derived immunosuppression in the TME.NATURE PORTFOLIO2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/18848Scientific ReportsISSN: 20452322reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p188482026-06-11T12:45:17Z
dc.title.none.fl_str_mv A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
title A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
spellingShingle A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
Piqueras-Nebot, M
Myeloid-derived suppressor cells
Human
Tumor-infiltrating
Isolation
Lung cancer
Immunosuppression
title_short A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
title_full A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
title_fullStr A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
title_full_unstemmed A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
title_sort A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue
dc.creator.none.fl_str_mv Piqueras-Nebot, M
Benet, M
Estors, M
Cremades, A
Juan-Vidal, O
Carretero, J
Galbis-Caravajal, JM
Lahoz, A
author Piqueras-Nebot, M
author_facet Piqueras-Nebot, M
Benet, M
Estors, M
Cremades, A
Juan-Vidal, O
Carretero, J
Galbis-Caravajal, JM
Lahoz, A
author_role author
author2 Benet, M
Estors, M
Cremades, A
Juan-Vidal, O
Carretero, J
Galbis-Caravajal, JM
Lahoz, A
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Myeloid-derived suppressor cells
Human
Tumor-infiltrating
Isolation
Lung cancer
Immunosuppression
topic Myeloid-derived suppressor cells
Human
Tumor-infiltrating
Isolation
Lung cancer
Immunosuppression
description The tumor microenvironment comprises different cell subsets including myeloid-derived suppressor cells, which exert intratumoral immunosuppression and favor cancer progression. Isolating tumor-infiltrating myeloid-derived suppressor cells (tMDSCs) from human tumor samples remains a challenge. Current methods such as magnetic bead sorting (MACS) or flow cytometry sorting (FACS) present some drawbacks in terms of purity and viability. Here, we have setup an innovative workflow that combines RosetteSep technology and MACS for isolation of tMDSCs from lung cancer biopsies. To evaluate our Rosette-MACS approach, we compared its performance with MACS and FACS. The isolated cells were characterized by flow cytometry, gene expression analysis and proliferation assays for comparison purposes. The results showed that the Rosette-MACS protocol had the highest yield and purity of tMDSCs (79.64% vs 13.30% with FACS and 0.39% with MACS). Furthermore, the functionality of the isolated tMDSCs was tested not only by upregulation of immunosuppressive genes (e.g. ARG1, IDO1, or PD-L1), but also by their capacity to inhibit CD8+ T cells proliferation. The combined use of RosetteSep and MACS provides an improved approach for the isolation of functional tMDSCs, which delineates a suitable experimental framework to selectively study the molecular mechanisms underpinning tMDSCs-derived immunosuppression in the TME.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/18848
url https://fisabio.portalinvestigacion.com/publicaciones/18848
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv NATURE PORTFOLIO
publisher.none.fl_str_mv NATURE PORTFOLIO
dc.source.none.fl_str_mv Scientific Reports
ISSN: 20452322
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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