The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process

Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky’s disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the...

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Autores: Timoneda, Oriol, Núñez-Hernández, Fernando, Balcells, Ingrid, Muñoz, Marta, Castelló, Anna, Vera, Gonzalo, Pérez, Lester Josué, Egea, Raquel, Mir, Gisela, Córdoba, Sarai, Rosell, Rosa, Segalés, Joaquim, Tomàs, Anna, Sánchez, Armand, Núñez, José I.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/250508
Acceso en línea:http://hdl.handle.net/10261/250508
Access Level:acceso abierto
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spelling The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection processTimoneda, OriolNúñez-Hernández, FernandoBalcells, IngridMuñoz, MartaCastelló, AnnaVera, GonzaloPérez, Lester JosuéEgea, RaquelMir, GiselaCórdoba, SaraiRosell, RosaSegalés, JoaquimTomàs, AnnaSánchez, ArmandNúñez, José I.Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky’s disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky’s disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host – pathogen interaction during viral infection.This work was supported by the projects AGL2007-66371-C02, AGL2010-22358-C02 and CDS2006-00007 from Ministerio de Ciencia e Innovación. OT is the recipient of a PhD fellowship (Programa de Formación al Personal Investigador – FPI) from the Spanish government.Peer reviewedPublic Library of ScienceMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/250508reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1371/journal.pone.0086965Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2505082026-05-22T06:33:51Z
dc.title.none.fl_str_mv The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
title The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
spellingShingle The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
Timoneda, Oriol
title_short The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
title_full The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
title_fullStr The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
title_full_unstemmed The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
title_sort The role of viral and host microRNAs in the Aujeszky’s disease virus during the infection process
dc.creator.none.fl_str_mv Timoneda, Oriol
Núñez-Hernández, Fernando
Balcells, Ingrid
Muñoz, Marta
Castelló, Anna
Vera, Gonzalo
Pérez, Lester Josué
Egea, Raquel
Mir, Gisela
Córdoba, Sarai
Rosell, Rosa
Segalés, Joaquim
Tomàs, Anna
Sánchez, Armand
Núñez, José I.
author Timoneda, Oriol
author_facet Timoneda, Oriol
Núñez-Hernández, Fernando
Balcells, Ingrid
Muñoz, Marta
Castelló, Anna
Vera, Gonzalo
Pérez, Lester Josué
Egea, Raquel
Mir, Gisela
Córdoba, Sarai
Rosell, Rosa
Segalés, Joaquim
Tomàs, Anna
Sánchez, Armand
Núñez, José I.
author_role author
author2 Núñez-Hernández, Fernando
Balcells, Ingrid
Muñoz, Marta
Castelló, Anna
Vera, Gonzalo
Pérez, Lester Josué
Egea, Raquel
Mir, Gisela
Córdoba, Sarai
Rosell, Rosa
Segalés, Joaquim
Tomàs, Anna
Sánchez, Armand
Núñez, José I.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Porcine production is a primary market in the world economy. Controlling swine diseases in the farm is essential in order to achieve the sector necessities. Aujeszky’s disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. microRNAs (miRNAs) are non-coding RNAs which modulates gene expression in animals, plants and viruses. With the aim of understanding miRNA roles during the Aujeszky’s disease virus [ADV] (also known as suid herpesvirus type 1 [SuHV-1]) infection, the expression profiles of host and viral miRNAs were determined through deep sequencing in SuHV-1 infected porcine cell line (PK-15) and in an animal experimental SuHV-1 infection with virulent (NIA-3) and attenuated (Begonia) strains. In the in vivo approach miR-206, miR-133a, miR-133b and miR-378 presented differential expression between virus strains infection. In the in vitro approach, most miRNAs were down-regulated in infected groups. miR-92a and miR-92b-3p were up-regulated in Begonia infected samples. Functional analysis of all this over expressed miRNAs during the infection revealed their association in pathways related to viral infection processes and immune response. Furthermore, 8 viral miRNAs were detected by stem loop RT-qPCR in both in vitro and in vivo approaches, presenting a gene regulatory network affecting 59 viral genes. Most described viral miRNAs were related to Large Latency Transcript (LLT) and to viral transcription activators EP0 and IE180, and also to regulatory genes regarding their important roles in the host – pathogen interaction during viral infection.
publishDate 2014
dc.date.none.fl_str_mv 2014
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/250508
url http://hdl.handle.net/10261/250508
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1371/journal.pone.0086965
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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