The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans

Hair cell (HC) loss by epithelial extrusion has been described to occur in the rodent vestibular system during chronic 3,3'-iminodipropionitrile (IDPN) ototoxicity. This is preceded by dismantlement of the calyceal junction in the contact between type I HC (HCI) and calyx afferent terminals. He...

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Autores: Maroto, Alberto F., Borrajo, Mireia, Prades, Sílvia, Callejo Castillo, Àngela, Amilibia, Emilio, Pérez-Grau, Marta, Roca Ribas, Francesc, Castellanos, Elisabeth, Barrallo Gimeno, Alejandro, Llorens i Baucells, Jordi
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/199433
Acesso em linha:https://hdl.handle.net/2445/199433
Access Level:acceso abierto
Palavra-chave:Epiteli
Cèl·lules acústiques
Rates (Animals de laboratori)
Epithelium
Hair cells
Rats as laboratory animals
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spelling The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humansMaroto, Alberto F.Borrajo, MireiaPrades, SílviaCallejo Castillo, ÀngelaAmilibia, EmilioPérez-Grau, MartaRoca Ribas, FrancescCastellanos, ElisabethBarrallo Gimeno, AlejandroLlorens i Baucells, JordiEpiteliCèl·lules acústiquesRates (Animals de laboratori)EpitheliumHair cellsRats as laboratory animalsHair cell (HC) loss by epithelial extrusion has been described to occur in the rodent vestibular system during chronic 3,3'-iminodipropionitrile (IDPN) ototoxicity. This is preceded by dismantlement of the calyceal junction in the contact between type I HC (HCI) and calyx afferent terminals. Here, we evaluated whether these phenomena have wider significance. First, we studied rats receiving seven different doses of streptomycin, ranging from 100 to 800 mg/kg/day, for 3 to 8 weeks. Streptomycin caused loss of vestibular function associated with partial loss of HCI and decreased expression of contactin-associated protein (CASPR1), denoting calyceal junction dismantlement, in the calyces encasing the surviving HCI. Additional molecular and ultrastructural data supported the conclusion that HC-calyx detachment precede HCI loss by extrusion. Animals allowed to survive after the treatment showed functional recuperation and rebuilding of the calyceal junction. Second, we evaluated human sensory epithelia obtained during therapeutic labyrinthectomies and trans-labyrinthine tumour excisions. Some samples showed abnormal CASPR1 label strongly suggestive of calyceal junction dismantlement. Therefore, reversible dismantlement of the vestibular calyceal junction may be a common response triggered by chronic stress, including ototoxic stress, before HCI loss. This may partly explain clinical observations of reversion in function loss after aminoglycoside exposure.Springer Verlag2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/199433Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1007/s00204-023-03518-zArchives of Toxicology, 2023, vol. 97, p. 1943-1961https://doi.org/10.1007/s00204-023-03518-zcc by (c) Maroto, Alberto F. et al., 2023https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1994332026-05-27T06:46:51Z
dc.title.none.fl_str_mv The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
title The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
spellingShingle The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
Maroto, Alberto F.
Epiteli
Cèl·lules acústiques
Rates (Animals de laboratori)
Epithelium
Hair cells
Rats as laboratory animals
title_short The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
title_full The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
title_fullStr The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
title_full_unstemmed The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
title_sort The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans
dc.creator.none.fl_str_mv Maroto, Alberto F.
Borrajo, Mireia
Prades, Sílvia
Callejo Castillo, Àngela
Amilibia, Emilio
Pérez-Grau, Marta
Roca Ribas, Francesc
Castellanos, Elisabeth
Barrallo Gimeno, Alejandro
Llorens i Baucells, Jordi
author Maroto, Alberto F.
author_facet Maroto, Alberto F.
Borrajo, Mireia
Prades, Sílvia
Callejo Castillo, Àngela
Amilibia, Emilio
Pérez-Grau, Marta
Roca Ribas, Francesc
Castellanos, Elisabeth
Barrallo Gimeno, Alejandro
Llorens i Baucells, Jordi
author_role author
author2 Borrajo, Mireia
Prades, Sílvia
Callejo Castillo, Àngela
Amilibia, Emilio
Pérez-Grau, Marta
Roca Ribas, Francesc
Castellanos, Elisabeth
Barrallo Gimeno, Alejandro
Llorens i Baucells, Jordi
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Epiteli
Cèl·lules acústiques
Rates (Animals de laboratori)
Epithelium
Hair cells
Rats as laboratory animals
topic Epiteli
Cèl·lules acústiques
Rates (Animals de laboratori)
Epithelium
Hair cells
Rats as laboratory animals
description Hair cell (HC) loss by epithelial extrusion has been described to occur in the rodent vestibular system during chronic 3,3'-iminodipropionitrile (IDPN) ototoxicity. This is preceded by dismantlement of the calyceal junction in the contact between type I HC (HCI) and calyx afferent terminals. Here, we evaluated whether these phenomena have wider significance. First, we studied rats receiving seven different doses of streptomycin, ranging from 100 to 800 mg/kg/day, for 3 to 8 weeks. Streptomycin caused loss of vestibular function associated with partial loss of HCI and decreased expression of contactin-associated protein (CASPR1), denoting calyceal junction dismantlement, in the calyces encasing the surviving HCI. Additional molecular and ultrastructural data supported the conclusion that HC-calyx detachment precede HCI loss by extrusion. Animals allowed to survive after the treatment showed functional recuperation and rebuilding of the calyceal junction. Second, we evaluated human sensory epithelia obtained during therapeutic labyrinthectomies and trans-labyrinthine tumour excisions. Some samples showed abnormal CASPR1 label strongly suggestive of calyceal junction dismantlement. Therefore, reversible dismantlement of the vestibular calyceal junction may be a common response triggered by chronic stress, including ototoxic stress, before HCI loss. This may partly explain clinical observations of reversion in function loss after aminoglycoside exposure.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/199433
url https://hdl.handle.net/2445/199433
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1007/s00204-023-03518-z
Archives of Toxicology, 2023, vol. 97, p. 1943-1961
https://doi.org/10.1007/s00204-023-03518-z
dc.rights.none.fl_str_mv cc by (c) Maroto, Alberto F. et al., 2023
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Maroto, Alberto F. et al., 2023
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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