Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae

Lactococcus garvieae is a main ichthyopathogen in rainbow trout (Oncorhynchus mykiss, Walbaum) farming, although bacteriocinogenic L. garvieae with antimicrobial activity against virulent strains of this species have also been identified. Some of the bacteriocins characterized, such as garvicin A (G...

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Autores: Feito Hermida, Javier, Araújo, Carlos, Arbulu, Sara, Contente, Diogo, Gómez Sala, Beatriz, Díaz-Formoso, Lara, Muñoz Atienza, Estefanía, Borrero Del Pino, Juan, Cintas Izarra, Luis Miguel, Hernández Cruza, Pablo Elpidio
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/72398
Acceso en línea:https://hdl.handle.net/20.500.14352/72398
Access Level:acceso abierto
Palabra clave:Bacteriocins
Garvicin
nisin
Lactococcus garvieae
Lactic acid bacteria (LAB)
Heterologous production
Lactococcus lactis
Probiotics
Paraprobiotics
Postbiotics
Microbiología (Biología)
2414 Microbiología
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network_name_str España
repository_id_str
spelling Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieaeFeito Hermida, JavierAraújo, CarlosArbulu, SaraContente, DiogoGómez Sala, BeatrizDíaz-Formoso, LaraMuñoz Atienza, EstefaníaBorrero Del Pino, JuanCintas Izarra, Luis MiguelHernández Cruza, Pablo ElpidioBacteriocinsGarvicinnisinLactococcus garvieaeLactic acid bacteria (LAB)Heterologous productionLactococcus lactisProbioticsParaprobioticsPostbioticsMicrobiología (Biología)2414 MicrobiologíaLactococcus garvieae is a main ichthyopathogen in rainbow trout (Oncorhynchus mykiss, Walbaum) farming, although bacteriocinogenic L. garvieae with antimicrobial activity against virulent strains of this species have also been identified. Some of the bacteriocins characterized, such as garvicin A (GarA) and garvicin Q (GarQ), may show potential for the control of the virulent L. garvieae in food, feed and other biotechnological applications. In this study, we report on the design of Lactococcus lactis strains that produce the bacteriocins GarA and/or GarQ, either alone or together with nisin A (NisA) or nisin Z (NisZ). Synthetic genes encoding the signal peptide of the lactococcal protein Usp45 (SPusp45), fused to mature GarA (lgnA) and/or mature GarQ (garQ) and their associated immunity genes (lgnI and garI, respectively), were cloned into the protein expression vectors pMG36c, which contains the P32 constitutive promoter, and pNZ8048c, which contains the inducible PnisA promoter. The transformation of recombinant vectors into lactococcal cells allowed for the production of GarA and/or GarQ by L. lactis subsp. cremoris NZ9000 and their co-production with NisA by Lactococcus lactis subsp. lactis DPC5598 and L. lactis subsp. lactis BB24. The strains L. lactis subsp. cremoris WA2-67 (pJFQI), a producer of GarQ and NisZ, and L. lactis subsp. cremoris WA2-67 (pJFQIAI), a producer of GarA, GarQ and NisZ, demonstrated the highest antimicrobial activity (5.1- to 10.7-fold and 17.3- to 68.2-fold, respectively) against virulent L. garvieae strains.MPDIUniversidad Complutense de Madrid20232023-03-0220232023-03-02journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/72398reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/723982026-06-02T12:44:21Z
dc.title.none.fl_str_mv Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
title Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
spellingShingle Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
Feito Hermida, Javier
Bacteriocins
Garvicin
nisin
Lactococcus garvieae
Lactic acid bacteria (LAB)
Heterologous production
Lactococcus lactis
Probiotics
Paraprobiotics
Postbiotics
Microbiología (Biología)
2414 Microbiología
title_short Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
title_full Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
title_fullStr Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
title_full_unstemmed Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
title_sort Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
dc.creator.none.fl_str_mv Feito Hermida, Javier
Araújo, Carlos
Arbulu, Sara
Contente, Diogo
Gómez Sala, Beatriz
Díaz-Formoso, Lara
Muñoz Atienza, Estefanía
Borrero Del Pino, Juan
Cintas Izarra, Luis Miguel
Hernández Cruza, Pablo Elpidio
author Feito Hermida, Javier
author_facet Feito Hermida, Javier
Araújo, Carlos
Arbulu, Sara
Contente, Diogo
Gómez Sala, Beatriz
Díaz-Formoso, Lara
Muñoz Atienza, Estefanía
Borrero Del Pino, Juan
Cintas Izarra, Luis Miguel
Hernández Cruza, Pablo Elpidio
author_role author
author2 Araújo, Carlos
Arbulu, Sara
Contente, Diogo
Gómez Sala, Beatriz
Díaz-Formoso, Lara
Muñoz Atienza, Estefanía
Borrero Del Pino, Juan
Cintas Izarra, Luis Miguel
Hernández Cruza, Pablo Elpidio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Bacteriocins
Garvicin
nisin
Lactococcus garvieae
Lactic acid bacteria (LAB)
Heterologous production
Lactococcus lactis
Probiotics
Paraprobiotics
Postbiotics
Microbiología (Biología)
2414 Microbiología
topic Bacteriocins
Garvicin
nisin
Lactococcus garvieae
Lactic acid bacteria (LAB)
Heterologous production
Lactococcus lactis
Probiotics
Paraprobiotics
Postbiotics
Microbiología (Biología)
2414 Microbiología
description Lactococcus garvieae is a main ichthyopathogen in rainbow trout (Oncorhynchus mykiss, Walbaum) farming, although bacteriocinogenic L. garvieae with antimicrobial activity against virulent strains of this species have also been identified. Some of the bacteriocins characterized, such as garvicin A (GarA) and garvicin Q (GarQ), may show potential for the control of the virulent L. garvieae in food, feed and other biotechnological applications. In this study, we report on the design of Lactococcus lactis strains that produce the bacteriocins GarA and/or GarQ, either alone or together with nisin A (NisA) or nisin Z (NisZ). Synthetic genes encoding the signal peptide of the lactococcal protein Usp45 (SPusp45), fused to mature GarA (lgnA) and/or mature GarQ (garQ) and their associated immunity genes (lgnI and garI, respectively), were cloned into the protein expression vectors pMG36c, which contains the P32 constitutive promoter, and pNZ8048c, which contains the inducible PnisA promoter. The transformation of recombinant vectors into lactococcal cells allowed for the production of GarA and/or GarQ by L. lactis subsp. cremoris NZ9000 and their co-production with NisA by Lactococcus lactis subsp. lactis DPC5598 and L. lactis subsp. lactis BB24. The strains L. lactis subsp. cremoris WA2-67 (pJFQI), a producer of GarQ and NisZ, and L. lactis subsp. cremoris WA2-67 (pJFQIAI), a producer of GarA, GarQ and NisZ, demonstrated the highest antimicrobial activity (5.1- to 10.7-fold and 17.3- to 68.2-fold, respectively) against virulent L. garvieae strains.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-03-02
2023
2023-03-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/72398
url https://hdl.handle.net/20.500.14352/72398
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MPDI
publisher.none.fl_str_mv MPDI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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