Long-read metagenomics retrieves complete single-contig bacterial genomes from canine feces
Long-read sequencing in metagenomics facilitates the assembly of complete genomes out of complex microbial communities. These genomes include essential biologic information such as the ribosomal genes or the mobile genetic elements, which are usually missed with short-reads. We applied long-read met...
| Autores: | , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:255554 |
| Acesso em linha: | https://ddd.uab.cat/record/255554 https://dx.doi.org/urn:doi:10.1186/s12864-021-07607-0 |
| Access Level: | acceso abierto |
| Palavra-chave: | Long-read metagenomics Gastrointestinal microbiome Fecal microbiome Long-reads Nanopore Canine microbiome Dog microbiome Metagenome-assembled genomes Sutterella |
| Resumo: | Long-read sequencing in metagenomics facilitates the assembly of complete genomes out of complex microbial communities. These genomes include essential biologic information such as the ribosomal genes or the mobile genetic elements, which are usually missed with short-reads. We applied long-read metagenomics with Nanopore sequencing to retrieve high-quality metagenome-assembled genomes (HQ MAGs) from a dog fecal sample. We used nanopore long-read metagenomics and frameshift aware correction on a canine fecal sample and retrieved eight single-contig HQ MAGs, which were. |
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