Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)

Despite the nutritional properties of alfalfa, its production is mainly for animal feed and it is undervalued as a food source. In this study, the valorization of alfalfa as a potential source of bioactive carbohydrates [inositols, α-galactooligosaccharides (α-GOS)] is presented. A Box– Behnken expe...

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Autores: Solarte,Daniela Alejandra, Ruiz-Matute, Ana I., Chito-Trujillo, Diana M., Rada-Mendoza, Maite, Sanz, M. Luz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/245749
Acceso en línea:http://hdl.handle.net/10261/245749
Access Level:acceso abierto
Palabra clave:Inositols
Sugars
Alpha-galactooligosaccharides (alpha-GOS)
Microwave assisted extraction (MAE)
Solid-liquid extraction (SLE)
Alfalfa (Medicago sativa L.)
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spelling Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)Solarte,Daniela AlejandraRuiz-Matute, Ana I.Chito-Trujillo, Diana M.Rada-Mendoza, MaiteSanz, M. LuzInositolsSugarsAlpha-galactooligosaccharides (alpha-GOS)Microwave assisted extraction (MAE)Solid-liquid extraction (SLE)Alfalfa (Medicago sativa L.)Despite the nutritional properties of alfalfa, its production is mainly for animal feed and it is undervalued as a food source. In this study, the valorization of alfalfa as a potential source of bioactive carbohydrates [inositols, α-galactooligosaccharides (α-GOS)] is presented. A Box– Behnken experimental design was used to optimize the extraction of these carbohydrates from leaves, stems, and seeds of alfalfa by solid–liquid extraction (SLE) and microwave-assisted extraction (MAE). Optimal extraction temperatures were similar for both treatments (40◦C leaves, 80◦C seeds); however, SLE required longer times (32.5 and 60 min vs. 5 min). In general, under similar extraction conditions, MAE provided higher yields of inositols (up to twice) and α-GOS (up to 7 times); hence, MAE was selected for their extraction from 13 alfalfa samples. Pinitol was the most abundant inositol of leaves and stems (24.2–31.0 mg·g−1 and 15.5–22.5 mg·g−1, respectively) while seed extracts were rich in α-GOS, mainly in stachyose (48.8–84.7 mg·g−1). In addition, inositols and α-GOS concentrations of lyophilized MAE extracts were stable for up to 26 days at 50◦C. These findings demonstrate that alfalfa is a valuable source of bioactive carbohydrates and MAE a promising alternative technique to obtain functional extracts.Authors thank the Spanish Ministry of Economy, Industry and Competitiveness (project AGL2016-80475-R, AEI/FEDER, UE), Ministry of Science and Innovation and Spanish State Research Agency (project reference PID2019-106405GB-I00/AEI/10.13039/501100011033), the Comunidad of Madrid and European funding from FSE and FEDER programs (project reference S2018/BAA-4393, AVANSECAL-II-CM) and to Universidad del Cauca (501100005682) of Colombia (project ID-4652) for financial support.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)European CommissionMinisterio de Ciencia, Innovación y Universidades (España)Comunidad de MadridUniversidad del CaucaRuiz-Matute, Ana Isabel [0000-0001-9143-8128]Luz Sanz, Maria [0000-0002-8156-4734]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/245749reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80475-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106405GB-I00S2018/BAA-4393, AVANSECAL-II-CMhttps://doi.org/10.3390/foods10020346Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2457492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
title Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
spellingShingle Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
Solarte,Daniela Alejandra
Inositols
Sugars
Alpha-galactooligosaccharides (alpha-GOS)
Microwave assisted extraction (MAE)
Solid-liquid extraction (SLE)
Alfalfa (Medicago sativa L.)
title_short Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
title_full Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
title_fullStr Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
title_full_unstemmed Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
title_sort Microwave Assisted Extraction of Bioactive Carbohydrates from Different Morphological Parts of Alfalfa (Medicago sativa L.)
dc.creator.none.fl_str_mv Solarte,Daniela Alejandra
Ruiz-Matute, Ana I.
Chito-Trujillo, Diana M.
Rada-Mendoza, Maite
Sanz, M. Luz
author Solarte,Daniela Alejandra
author_facet Solarte,Daniela Alejandra
Ruiz-Matute, Ana I.
Chito-Trujillo, Diana M.
Rada-Mendoza, Maite
Sanz, M. Luz
author_role author
author2 Ruiz-Matute, Ana I.
Chito-Trujillo, Diana M.
Rada-Mendoza, Maite
Sanz, M. Luz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Comunidad de Madrid
Universidad del Cauca
Ruiz-Matute, Ana Isabel [0000-0001-9143-8128]
Luz Sanz, Maria [0000-0002-8156-4734]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Inositols
Sugars
Alpha-galactooligosaccharides (alpha-GOS)
Microwave assisted extraction (MAE)
Solid-liquid extraction (SLE)
Alfalfa (Medicago sativa L.)
topic Inositols
Sugars
Alpha-galactooligosaccharides (alpha-GOS)
Microwave assisted extraction (MAE)
Solid-liquid extraction (SLE)
Alfalfa (Medicago sativa L.)
description Despite the nutritional properties of alfalfa, its production is mainly for animal feed and it is undervalued as a food source. In this study, the valorization of alfalfa as a potential source of bioactive carbohydrates [inositols, α-galactooligosaccharides (α-GOS)] is presented. A Box– Behnken experimental design was used to optimize the extraction of these carbohydrates from leaves, stems, and seeds of alfalfa by solid–liquid extraction (SLE) and microwave-assisted extraction (MAE). Optimal extraction temperatures were similar for both treatments (40◦C leaves, 80◦C seeds); however, SLE required longer times (32.5 and 60 min vs. 5 min). In general, under similar extraction conditions, MAE provided higher yields of inositols (up to twice) and α-GOS (up to 7 times); hence, MAE was selected for their extraction from 13 alfalfa samples. Pinitol was the most abundant inositol of leaves and stems (24.2–31.0 mg·g−1 and 15.5–22.5 mg·g−1, respectively) while seed extracts were rich in α-GOS, mainly in stachyose (48.8–84.7 mg·g−1). In addition, inositols and α-GOS concentrations of lyophilized MAE extracts were stable for up to 26 days at 50◦C. These findings demonstrate that alfalfa is a valuable source of bioactive carbohydrates and MAE a promising alternative technique to obtain functional extracts.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/245749
url http://hdl.handle.net/10261/245749
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80475-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106405GB-I00
S2018/BAA-4393, AVANSECAL-II-CM
https://doi.org/10.3390/foods10020346
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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