Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas

Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213...

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Autores: Sprankel, Lasse, Vizarraga, David|||0000-0002-4478-0155, Martín Sánchez, Jesús|||0000-0003-4250-4824, Manger, Sina, Meier-Credo, Jakob|||0000-0003-1026-1573, Marcos, Marina, Julve i Gil, Josep|||0000-0002-6531-2246, Rotllan, Noemi|||0000-0002-0587-8045, Scheffer, Margot P., Escolà-Gil, Joan Carles|||0000-0001-9021-2485, Langer, Julian D.|||0000-0002-5190-577X, Piñol Ribas, Jaume|||0000-0002-9055-8934, Fita, Ignacio|||0000-0002-7521-2679, Frangakis, Achilleas S.|||0000-0002-8067-6611
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:280935
Acceso en línea:https://ddd.uab.cat/record/280935
https://dx.doi.org/urn:doi:10.1038/s41594-023-00922-y
Access Level:acceso abierto
Palabra clave:Cryoelectron microscopy
Pathogens
Structural biology
Membrane lipids
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spelling Essential protein P116 extracts cholesterol and other indispensable lipids for MycoplasmasSprankel, LasseVizarraga, David|||0000-0002-4478-0155Martín Sánchez, Jesús|||0000-0003-4250-4824Manger, SinaMeier-Credo, Jakob|||0000-0003-1026-1573Marcos, MarinaJulve i Gil, Josep|||0000-0002-6531-2246Rotllan, Noemi|||0000-0002-0587-8045Scheffer, Margot P.Escolà-Gil, Joan Carles|||0000-0001-9021-2485Langer, Julian D.|||0000-0002-5190-577XPiñol Ribas, Jaume|||0000-0002-9055-8934Fita, Ignacio|||0000-0002-7521-2679Frangakis, Achilleas S.|||0000-0002-8067-6611Cryoelectron microscopyPathogensStructural biologyMembrane lipidsMycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213). Single-particle cryo-electron microscopy of P116 reveals a homodimer presenting a previously unseen fold, forming a huge hydrophobic cavity, which is fully accessible to solvent. Lipidomics analysis shows that P116 specifically extracts lipids such as phosphatidylcholine, sphingomyelin and cholesterol. Structures of different conformational states reveal the mechanism by which lipids are extracted. This finding immediately suggests a way to control Mycoplasma infection by interfering with lipid uptake. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/280935https://dx.doi.org/urn:doi:10.1038/s41594-023-00922-yreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 BIO2017-84166-RAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125632OB-C22Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125632OB-C21open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2809352026-06-06T12:50:31Z
dc.title.none.fl_str_mv Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
title Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
spellingShingle Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
Sprankel, Lasse
Cryoelectron microscopy
Pathogens
Structural biology
Membrane lipids
title_short Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
title_full Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
title_fullStr Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
title_full_unstemmed Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
title_sort Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
dc.creator.none.fl_str_mv Sprankel, Lasse
Vizarraga, David|||0000-0002-4478-0155
Martín Sánchez, Jesús|||0000-0003-4250-4824
Manger, Sina
Meier-Credo, Jakob|||0000-0003-1026-1573
Marcos, Marina
Julve i Gil, Josep|||0000-0002-6531-2246
Rotllan, Noemi|||0000-0002-0587-8045
Scheffer, Margot P.
Escolà-Gil, Joan Carles|||0000-0001-9021-2485
Langer, Julian D.|||0000-0002-5190-577X
Piñol Ribas, Jaume|||0000-0002-9055-8934
Fita, Ignacio|||0000-0002-7521-2679
Frangakis, Achilleas S.|||0000-0002-8067-6611
author Sprankel, Lasse
author_facet Sprankel, Lasse
Vizarraga, David|||0000-0002-4478-0155
Martín Sánchez, Jesús|||0000-0003-4250-4824
Manger, Sina
Meier-Credo, Jakob|||0000-0003-1026-1573
Marcos, Marina
Julve i Gil, Josep|||0000-0002-6531-2246
Rotllan, Noemi|||0000-0002-0587-8045
Scheffer, Margot P.
Escolà-Gil, Joan Carles|||0000-0001-9021-2485
Langer, Julian D.|||0000-0002-5190-577X
Piñol Ribas, Jaume|||0000-0002-9055-8934
Fita, Ignacio|||0000-0002-7521-2679
Frangakis, Achilleas S.|||0000-0002-8067-6611
author_role author
author2 Vizarraga, David|||0000-0002-4478-0155
Martín Sánchez, Jesús|||0000-0003-4250-4824
Manger, Sina
Meier-Credo, Jakob|||0000-0003-1026-1573
Marcos, Marina
Julve i Gil, Josep|||0000-0002-6531-2246
Rotllan, Noemi|||0000-0002-0587-8045
Scheffer, Margot P.
Escolà-Gil, Joan Carles|||0000-0001-9021-2485
Langer, Julian D.|||0000-0002-5190-577X
Piñol Ribas, Jaume|||0000-0002-9055-8934
Fita, Ignacio|||0000-0002-7521-2679
Frangakis, Achilleas S.|||0000-0002-8067-6611
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryoelectron microscopy
Pathogens
Structural biology
Membrane lipids
topic Cryoelectron microscopy
Pathogens
Structural biology
Membrane lipids
description Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213). Single-particle cryo-electron microscopy of P116 reveals a homodimer presenting a previously unseen fold, forming a huge hydrophobic cavity, which is fully accessible to solvent. Lipidomics analysis shows that P116 specifically extracts lipids such as phosphatidylcholine, sphingomyelin and cholesterol. Structures of different conformational states reveal the mechanism by which lipids are extracted. This finding immediately suggests a way to control Mycoplasma infection by interfering with lipid uptake.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/280935
https://dx.doi.org/urn:doi:10.1038/s41594-023-00922-y
url https://ddd.uab.cat/record/280935
https://dx.doi.org/urn:doi:10.1038/s41594-023-00922-y
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 BIO2017-84166-R
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125632OB-C22
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125632OB-C21
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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