The complexity of the calretinin-expressing progenitors in the human cerebral cortex

The complex structure and function of the cerebral cortex critically depend on the balance of excitation and inhibition provided by the pyramidal projection neurons and GABAergic interneurons, respectively.The calretinin-expressing (CalR+) cell is a subtype of GABAergic cortical interneurons that is...

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Autores: Radonjic, Nevena V., Ortega Cano, Juan Alberto, Memi, Fani, Dionne, Krista, Dionne K, Jakovcevski, Igor, Zecevic, Nada
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/187433
Acceso en línea:https://hdl.handle.net/2445/187433
Access Level:acceso abierto
Palabra clave:Escorça cerebral
Proteïnes
Neurones
Cerebral cortex
Proteins
Neurons
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spelling The complexity of the calretinin-expressing progenitors in the human cerebral cortexRadonjic, Nevena V.Ortega Cano, Juan AlbertoMemi, FaniDionne, KristaDionne KJakovcevski, IgorZecevic, NadaEscorça cerebralProteïnesNeuronesCerebral cortexProteinsNeuronsThe complex structure and function of the cerebral cortex critically depend on the balance of excitation and inhibition provided by the pyramidal projection neurons and GABAergic interneurons, respectively.The calretinin-expressing (CalR+) cell is a subtype of GABAergic cortical interneurons that is more prevalent in humans than in rodents. In rodents, CalR+ interneurons originate in the caudal ganglionic eminence (CGE) from Gsx2+ progenitors, but in humans it has been suggested that a subpopulation of CalR+ cells can also be generated in the cortical ventricular/subventricular zone (VZ/SVZ). The progenitors for cortically generated CalR+ subpopulation in primates are not yet characterized. Hence, the aim of this study was to identify patterns of expression of the transcription factors (TFs) that commit cortical stem cells to the CalR fate, with a focus on Gsx2. First, we studied the expression of Gsx2 and its downstream effectors, Ascl1 and Sp8 in the cortical regions of the fetal human forebrain at midgestation. Next, we established that a subpopulation of cells expressing these TFs are proliferating in the cortical SVZ, and can be co-labeled with CalR. The presence and proliferation of Gsx2+ cells, not only in the ventral telencephalon (GE) as previously reported, but also in the cerebral cortex suggests cortical origin of a subpopulation of CalR+ neurons in humans. In vitro treatment of human cortical progenitors with Sonic hedgehog (Shh), an important morphogen in the specification of interneurons, decreased levels of Ascl1 and Sp8 proteins, but did not affect Gsx2 levels. Taken together, our ex-vivo and in vitro results on human fetal brain suggest complex endogenous and exogenous regulation of TFs implied in the specification of different subtypes of CalR+ cortical interneurons.Frontiers Media2022202220142022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/187433Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fnana.2014.00082Frontiers In Neuroanatomy, 2014, vol. 8, num. 82https://doi.org/10.3389/fnana.2014.00082cc-by (c) Radonjic, Nevena V. et al., 2014https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1874332026-05-29T05:05:01Z
dc.title.none.fl_str_mv The complexity of the calretinin-expressing progenitors in the human cerebral cortex
title The complexity of the calretinin-expressing progenitors in the human cerebral cortex
spellingShingle The complexity of the calretinin-expressing progenitors in the human cerebral cortex
Radonjic, Nevena V.
Escorça cerebral
Proteïnes
Neurones
Cerebral cortex
Proteins
Neurons
title_short The complexity of the calretinin-expressing progenitors in the human cerebral cortex
title_full The complexity of the calretinin-expressing progenitors in the human cerebral cortex
title_fullStr The complexity of the calretinin-expressing progenitors in the human cerebral cortex
title_full_unstemmed The complexity of the calretinin-expressing progenitors in the human cerebral cortex
title_sort The complexity of the calretinin-expressing progenitors in the human cerebral cortex
dc.creator.none.fl_str_mv Radonjic, Nevena V.
Ortega Cano, Juan Alberto
Memi, Fani
Dionne, Krista
Dionne K
Jakovcevski, Igor
Zecevic, Nada
author Radonjic, Nevena V.
author_facet Radonjic, Nevena V.
Ortega Cano, Juan Alberto
Memi, Fani
Dionne, Krista
Dionne K
Jakovcevski, Igor
Zecevic, Nada
author_role author
author2 Ortega Cano, Juan Alberto
Memi, Fani
Dionne, Krista
Dionne K
Jakovcevski, Igor
Zecevic, Nada
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Escorça cerebral
Proteïnes
Neurones
Cerebral cortex
Proteins
Neurons
topic Escorça cerebral
Proteïnes
Neurones
Cerebral cortex
Proteins
Neurons
description The complex structure and function of the cerebral cortex critically depend on the balance of excitation and inhibition provided by the pyramidal projection neurons and GABAergic interneurons, respectively.The calretinin-expressing (CalR+) cell is a subtype of GABAergic cortical interneurons that is more prevalent in humans than in rodents. In rodents, CalR+ interneurons originate in the caudal ganglionic eminence (CGE) from Gsx2+ progenitors, but in humans it has been suggested that a subpopulation of CalR+ cells can also be generated in the cortical ventricular/subventricular zone (VZ/SVZ). The progenitors for cortically generated CalR+ subpopulation in primates are not yet characterized. Hence, the aim of this study was to identify patterns of expression of the transcription factors (TFs) that commit cortical stem cells to the CalR fate, with a focus on Gsx2. First, we studied the expression of Gsx2 and its downstream effectors, Ascl1 and Sp8 in the cortical regions of the fetal human forebrain at midgestation. Next, we established that a subpopulation of cells expressing these TFs are proliferating in the cortical SVZ, and can be co-labeled with CalR. The presence and proliferation of Gsx2+ cells, not only in the ventral telencephalon (GE) as previously reported, but also in the cerebral cortex suggests cortical origin of a subpopulation of CalR+ neurons in humans. In vitro treatment of human cortical progenitors with Sonic hedgehog (Shh), an important morphogen in the specification of interneurons, decreased levels of Ascl1 and Sp8 proteins, but did not affect Gsx2 levels. Taken together, our ex-vivo and in vitro results on human fetal brain suggest complex endogenous and exogenous regulation of TFs implied in the specification of different subtypes of CalR+ cortical interneurons.
publishDate 2014
dc.date.none.fl_str_mv 2014
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/187433
url https://hdl.handle.net/2445/187433
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fnana.2014.00082
Frontiers In Neuroanatomy, 2014, vol. 8, num. 82
https://doi.org/10.3389/fnana.2014.00082
dc.rights.none.fl_str_mv cc-by (c) Radonjic, Nevena V. et al., 2014
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Radonjic, Nevena V. et al., 2014
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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