Label-free optical biosensing using low-cost electrospun polymeric nanofibers

Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry...

Descripción completa

Detalles Bibliográficos
Autores: Martínez Pérez, P., Ponce Alcántara, S., Murillo, Nieves, Pérez Márquez, Ana, Maudes, J., Peraile, Inés, González López, L., Gil García, M., Lorenzo Lozano, P., García Rupérez, J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Instituto Nacional de Técnica Aeroespacial (INTA)
Repositorio:DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
OAI Identifier:oai:digital.inta.es:20.500.12666/427
Acceso en línea:https://www.mdpi.com/2227-9040/8/4/119
http://hdl.handle.net/20.500.12666/427
Access Level:acceso abierto
Palabra clave:Electrospinning
Nanofibers
Polycaprolactone
Optical biosensors
id ES_2aa309946db4dff628a4bec18645ba28
oai_identifier_str oai:digital.inta.es:20.500.12666/427
network_acronym_str ES
network_name_str España
repository_id_str
spelling Label-free optical biosensing using low-cost electrospun polymeric nanofibersMartínez Pérez, P.Ponce Alcántara, S.Murillo, NievesPérez Márquez, AnaMaudes, J.Peraile, InésGonzález López, L.Gil García, M.Lorenzo Lozano, P.García Rupérez, J.ElectrospinningNanofibersPolycaprolactoneOptical biosensorsPolymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.This research was supported by a co-financed action by the European Union through the operational program of the European Regional Development Fund (FEDER) of the Valencian Community 2014–2020, the Generalitat Valenciana through the PROMETEO project AVANTI/2019/123 and the grant PPC/2020/037, the Spanish government through the project TEC2015-63838-C3-OPTONANOSENS, Universitat Politècnica de València through grant PAID-01-17, and by the Basque government through the project µ4Industry, KK-2019/00101, from the ELKARTEK Program; With funding from the Spanish government through the "María de Maeztu Unit of Excellence" accreditation (MDM-2017-0737).MDPI AgUnidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737Generalitat Valenciana (GVA)Ministerio de Economía y Competitividad (MINECO)202120212020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501application/pdfhttps://www.mdpi.com/2227-9040/8/4/119http://hdl.handle.net/20.500.12666/427reponame:DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacialinstname:Instituto Nacional de Técnica Aeroespacial (INTA)Inglésinfo:eu-repo/grantAgreement/MINECO//TEC2015-63838-C3-1-RAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:digital.inta.es:20.500.12666/4272026-06-23T12:46:37Z
dc.title.none.fl_str_mv Label-free optical biosensing using low-cost electrospun polymeric nanofibers
title Label-free optical biosensing using low-cost electrospun polymeric nanofibers
spellingShingle Label-free optical biosensing using low-cost electrospun polymeric nanofibers
Martínez Pérez, P.
Electrospinning
Nanofibers
Polycaprolactone
Optical biosensors
title_short Label-free optical biosensing using low-cost electrospun polymeric nanofibers
title_full Label-free optical biosensing using low-cost electrospun polymeric nanofibers
title_fullStr Label-free optical biosensing using low-cost electrospun polymeric nanofibers
title_full_unstemmed Label-free optical biosensing using low-cost electrospun polymeric nanofibers
title_sort Label-free optical biosensing using low-cost electrospun polymeric nanofibers
dc.creator.none.fl_str_mv Martínez Pérez, P.
Ponce Alcántara, S.
Murillo, Nieves
Pérez Márquez, Ana
Maudes, J.
Peraile, Inés
González López, L.
Gil García, M.
Lorenzo Lozano, P.
García Rupérez, J.
author Martínez Pérez, P.
author_facet Martínez Pérez, P.
Ponce Alcántara, S.
Murillo, Nieves
Pérez Márquez, Ana
Maudes, J.
Peraile, Inés
González López, L.
Gil García, M.
Lorenzo Lozano, P.
García Rupérez, J.
author_role author
author2 Ponce Alcántara, S.
Murillo, Nieves
Pérez Márquez, Ana
Maudes, J.
Peraile, Inés
González López, L.
Gil García, M.
Lorenzo Lozano, P.
García Rupérez, J.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
Generalitat Valenciana (GVA)
Ministerio de Economía y Competitividad (MINECO)
dc.subject.none.fl_str_mv Electrospinning
Nanofibers
Polycaprolactone
Optical biosensors
topic Electrospinning
Nanofibers
Polycaprolactone
Optical biosensors
description Polymeric nanofiber matrices are promising structures to develop biosensing devices due to their easy and affordable large-scale fabrication and their high surface-to-volume ratio. In this work, the suitability of a polyamide 6 nanofiber matrix for the development of a label-free and real-time Fabry–Pérot cavity-based optical biosensor was studied. For such aim, in-flow biofunctionalization of nanofibers with antibodies, bound through a protein A/G layer, and specific biodetection of 10 µg/mL bovine serum albumin (BSA) were carried out. Both processes were successfully monitored via reflectivity measurements in real-time without labels and their reproducibility was demonstrated when different polymeric nanofiber matrices from the same electrospinning batch were employed as transducers. These results demonstrate not only the suitability of correctly biofunctionalized polyamide 6 nanofiber matrices to be employed for real-time and label-free specific biodetection purposes, but also the potential of electrospinning technique to create affordable and easy-to-fabricate at large scale optical transducers with a reproducible performance.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/2227-9040/8/4/119
http://hdl.handle.net/20.500.12666/427
url https://www.mdpi.com/2227-9040/8/4/119
http://hdl.handle.net/20.500.12666/427
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//TEC2015-63838-C3-1-R
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI Ag
publisher.none.fl_str_mv MDPI Ag
dc.source.none.fl_str_mv reponame:DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
instname:Instituto Nacional de Técnica Aeroespacial (INTA)
instname_str Instituto Nacional de Técnica Aeroespacial (INTA)
reponame_str DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
collection DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405085009707008
score 15,301603

Similares en LA Referencia