Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples

The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc lucifer...

Descripción completa

Detalles Bibliográficos
Autores: Luque Uria, Álvaro, Peltomaa, Riikka Johanna, Navarro Duro, Marina, Fikacek, Sabrina, Head, Trajen, Deo, Sapna K., Daunert, Sylvia, Benito Peña, María Elena, Moreno Bondi, María Cruz
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/71361
Acceso en línea:https://hdl.handle.net/20.500.14352/71361
Access Level:acceso abierto
Palabra clave:543
bioluminescence
fumonisin B1
fusion proteins
immunoassays
luciferases
Química analítica (Química)
2301 Química Analítica
id ES_2a7fa2aca097155c92cbdb5bec05ee5d
oai_identifier_str oai:docta.ucm.es:20.500.14352/71361
network_acronym_str ES
network_name_str España
repository_id_str
spelling Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat SamplesLuque Uria, ÁlvaroPeltomaa, Riikka JohannaNavarro Duro, MarinaFikacek, SabrinaHead, TrajenDeo, Sapna K.Daunert, SylviaBenito Peña, María ElenaMoreno Bondi, María Cruz543bioluminescencefumonisin B1fusion proteinsimmunoassaysluciferasesQuímica analítica (Química)2301 Química AnalíticaThe development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.Wiley-VCHUniversidad Complutense de Madrid20222022-02-1720222022-02-17journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/71361reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/713612026-06-02T12:44:21Z
dc.title.none.fl_str_mv Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
title Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
spellingShingle Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
Luque Uria, Álvaro
543
bioluminescence
fumonisin B1
fusion proteins
immunoassays
luciferases
Química analítica (Química)
2301 Química Analítica
title_short Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
title_full Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
title_fullStr Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
title_full_unstemmed Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
title_sort Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
dc.creator.none.fl_str_mv Luque Uria, Álvaro
Peltomaa, Riikka Johanna
Navarro Duro, Marina
Fikacek, Sabrina
Head, Trajen
Deo, Sapna K.
Daunert, Sylvia
Benito Peña, María Elena
Moreno Bondi, María Cruz
author Luque Uria, Álvaro
author_facet Luque Uria, Álvaro
Peltomaa, Riikka Johanna
Navarro Duro, Marina
Fikacek, Sabrina
Head, Trajen
Deo, Sapna K.
Daunert, Sylvia
Benito Peña, María Elena
Moreno Bondi, María Cruz
author_role author
author2 Peltomaa, Riikka Johanna
Navarro Duro, Marina
Fikacek, Sabrina
Head, Trajen
Deo, Sapna K.
Daunert, Sylvia
Benito Peña, María Elena
Moreno Bondi, María Cruz
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
bioluminescence
fumonisin B1
fusion proteins
immunoassays
luciferases
Química analítica (Química)
2301 Química Analítica
topic 543
bioluminescence
fumonisin B1
fusion proteins
immunoassays
luciferases
Química analítica (Química)
2301 Química Analítica
description The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-02-17
2022
2022-02-17
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/71361
url https://hdl.handle.net/20.500.14352/71361
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405072254828544
score 15.301603

Similares en LA Referencia