Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification

10 pages, 2 figures, 3 tables

Detalles Bibliográficos
Autores: Roumani, Foteini, Gómez, Saioa, Prado, Marta, Garrido-Maestu, Alejandro
Tipo de recurso: otro
Estado:Versión aceptada para publicación
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/397280
Acceso en línea:http://hdl.handle.net/10261/397280
Access Level:acceso embargado
Palabra clave:LAMP
Fungi
Colorimetric detection
Naked eye
Real-time fluorescence
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spelling Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal AmplificationRoumani, FoteiniGómez, SaioaPrado, MartaGarrido-Maestu, AlejandroLAMPFungiColorimetric detectionNaked eyeReal-time fluorescence10 pages, 2 figures, 3 tablesMolecular DNA-based methods, such as PCR/qPCR, have been employed to detect yeasts and molds in various food products, offering faster results due to their heightened sensitivity and specificity, compared to culture-based techniques. However, recently, isothermal amplification techniques like loop-mediated isothermal amplification (LAMP) have emerged, simplifying assays and reducing costs. In this chapter a panfungal LAMP assay (LAMP-18S) for the rapid detection of spoilage responsible fungi in fruit preparations is described. Primers targeting the 18S rRNA gene were designed for this purpose. The study explored two distinct detection strategies: real-time fluorescence and naked-eye color detectionPeer reviewedSpringer NatureConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248Postprintinfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/397280reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésMethods and Protocols in Food Sciencehttps://doi.org/10.1007/978-1-0716-4526-0_14Síinfo:eu-repo/semantics/embargoedAccessoai:digital.csic.es:10261/3972802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
title Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
spellingShingle Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
Roumani, Foteini
LAMP
Fungi
Colorimetric detection
Naked eye
Real-time fluorescence
title_short Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
title_full Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
title_fullStr Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
title_full_unstemmed Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
title_sort Detection of Spoilage Fungi in Foods by Loop-Mediated Isothermal Amplification
dc.creator.none.fl_str_mv Roumani, Foteini
Gómez, Saioa
Prado, Marta
Garrido-Maestu, Alejandro
author Roumani, Foteini
author_facet Roumani, Foteini
Gómez, Saioa
Prado, Marta
Garrido-Maestu, Alejandro
author_role author
author2 Gómez, Saioa
Prado, Marta
Garrido-Maestu, Alejandro
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv LAMP
Fungi
Colorimetric detection
Naked eye
Real-time fluorescence
topic LAMP
Fungi
Colorimetric detection
Naked eye
Real-time fluorescence
description 10 pages, 2 figures, 3 tables
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
Postprint
info:eu-repo/semantics/acceptedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/397280
url http://hdl.handle.net/10261/397280
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Methods and Protocols in Food Science
https://doi.org/10.1007/978-1-0716-4526-0_14
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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