Epigenetic changes accompany developmental programmed cell death in tapetum cells

10 páginas, 8 figuras --PAGS nros. 16-29(2014)

Detalles Bibliográficos
Autores: Solís, María Teresa, Chakrabartin, Nandine, Corredor, Eduardo, Cortés-Eslava, Josefina, Rodríguez-Serrano, María, Biggiogera, Marco, Risueño, María Carmen, Testillano, Pilar S.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/89741
Acceso en línea:http://hdl.handle.net/10261/89741
Access Level:acceso abierto
Palabra clave:Anther development
Brassica napus
DNA methylation
DNA methyltransferase
5-Methyldeoxycytosine
Nicotiana tabacum
id ES_2a58f08bd68c84a65d58ee30cf6917bf
oai_identifier_str oai:digital.csic.es:10261/89741
network_acronym_str ES
network_name_str España
repository_id_str
spelling Epigenetic changes accompany developmental programmed cell death in tapetum cellsSolís, María TeresaChakrabartin, NandineCorredor, EduardoCortés-Eslava, JosefinaRodríguez-Serrano, MaríaBiggiogera, MarcoRisueño, María CarmenTestillano, Pilar S.Anther developmentBrassica napusDNA methylationDNA methyltransferase5-MethyldeoxycytosineNicotiana tabacum10 páginas, 8 figuras --PAGS nros. 16-29(2014)The tapetum, the nursing tissue inside anthers, undergoes cellular degradation by programmed cell death (PCD) during late stages of microspore–early pollen development. Despite the key function of tapetum, little is known about the molecular mechanisms regulating this cell death process in which profound nuclear and chromatin changes occur. Epigenetic features (DNA methylation and histone modifications) have been revealed as hallmarks that establish the functional status of chromatin domains, but no evidence on the epigenetic regulation of PCD has been reported. DNA methylation is accomplished by DNA methyltransferases, among which DNA methyl transferase 1 (MET1) constitutes one of the CG maintenance methyltransferase in plants, also showing de novo methyltransferase activity. In this work, the changes in epigenetic marks during the PCD of tapetal cells have been investigated by a multidisciplinary approach to reveal the dynamics of DNA methylation and the pattern of expression of MET1 in relation to the main cellular changes of this PCD process which have also been characterized in two species, Brassica napus and Nicotiana tabacum. The results showed that tapetum PCD progresses with the increase in global DNA methylation and MET1 expression, epigenetic changes that accompanied the reorganization of the nuclear architecture and a high chromatin condensation, activity of caspase 3-like proteases and Cyt c release. The reported data indicate a relationship between the PCD process and the DNA methylation dynamics and MET1 expression in tapetal cells, suggesting a possible new role for the epigenetic marks in the nuclear events occurring during this cell death process and providing new insights into the epigenetic control of plant PCDThis work was supported by the Spanish Ministry of Economy and Competitivity, MINECO [BFU2011-23752]; the Spanish National Research Council, CSIC [201020E038]; the Spanish Ministry of Science and Innovation, MICINN [grant to N.C. for Stays of Foreign Postdoctoral Researchers and grant to J.C.E. for Sabaticcal Stays in Spanish Research Centre]; the Spanish Ministry of Science and Innovation, MICINN [a post-doctoral Juan-de-la-Cierva grant (JCI-2007-123-1177) to M.R.S.]Oxford University PressConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/89741reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/pcp/pct152Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/897412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Epigenetic changes accompany developmental programmed cell death in tapetum cells
title Epigenetic changes accompany developmental programmed cell death in tapetum cells
spellingShingle Epigenetic changes accompany developmental programmed cell death in tapetum cells
Solís, María Teresa
Anther development
Brassica napus
DNA methylation
DNA methyltransferase
5-Methyldeoxycytosine
Nicotiana tabacum
title_short Epigenetic changes accompany developmental programmed cell death in tapetum cells
title_full Epigenetic changes accompany developmental programmed cell death in tapetum cells
title_fullStr Epigenetic changes accompany developmental programmed cell death in tapetum cells
title_full_unstemmed Epigenetic changes accompany developmental programmed cell death in tapetum cells
title_sort Epigenetic changes accompany developmental programmed cell death in tapetum cells
dc.creator.none.fl_str_mv Solís, María Teresa
Chakrabartin, Nandine
Corredor, Eduardo
Cortés-Eslava, Josefina
Rodríguez-Serrano, María
Biggiogera, Marco
Risueño, María Carmen
Testillano, Pilar S.
author Solís, María Teresa
author_facet Solís, María Teresa
Chakrabartin, Nandine
Corredor, Eduardo
Cortés-Eslava, Josefina
Rodríguez-Serrano, María
Biggiogera, Marco
Risueño, María Carmen
Testillano, Pilar S.
author_role author
author2 Chakrabartin, Nandine
Corredor, Eduardo
Cortés-Eslava, Josefina
Rodríguez-Serrano, María
Biggiogera, Marco
Risueño, María Carmen
Testillano, Pilar S.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anther development
Brassica napus
DNA methylation
DNA methyltransferase
5-Methyldeoxycytosine
Nicotiana tabacum
topic Anther development
Brassica napus
DNA methylation
DNA methyltransferase
5-Methyldeoxycytosine
Nicotiana tabacum
description 10 páginas, 8 figuras --PAGS nros. 16-29(2014)
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/89741
url http://hdl.handle.net/10261/89741
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/pcp/pct152
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405063039942656
score 15,198674

Similares en LA Referencia