Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning

Protein patterning is of interest in high-throughput screening. Due to an increase in demand for further miniaturization of protein assays, block copolymers (BCPs) that can undergo large-area phase separation into nanometer-size domains have attracted great attention as substrates for protein nanopa...

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Detalles Bibliográficos
Autores: Lagunas, Anna, Sasso, B., Tesson, N., Cantos, Carme, Martínez Fraiz, Elena, Samitier i Martí, Josep
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/121071
Acceso en línea:https://hdl.handle.net/2445/121071
Access Level:acceso abierto
Palabra clave:Copolímers
Nanoestructures
Nanotecnologia
Copolymers
Nanostructures
Nanotechnology
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spelling Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterningLagunas, AnnaSasso, B.Tesson, N.Cantos, CarmeMartínez Fraiz, ElenaSamitier i Martí, JosepCopolímersNanoestructuresNanotecnologiaCopolymersNanostructuresNanotechnologyProtein patterning is of interest in high-throughput screening. Due to an increase in demand for further miniaturization of protein assays, block copolymers (BCPs) that can undergo large-area phase separation into nanometer-size domains have attracted great attention as substrates for protein nanopatterning. Here we report the synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer which, once spin-coated, is capable of self-segregating into cylindrical polystyrene (PS) domains. In this copolymer, the PS block was modified to introduce biotin below 10% molar in order to achieve molecular recognition of streptavidin. The PMMA matrix used to introduce poly(ethylene glycol) enabled us to obtain an antifouling environment that prevents unspecific protein adsorption outside the domains. The use of the biotin-streptavidin pair in this BCP makes it suitable for nanopatterning of other biotinylated proteins of interest for the purposes of cell biology, biosensors, and tissue engineering.Royal Society of Chemistry2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/121071Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1039/C5PY01601KPolymer Chemistry, 2016, vol. 7, num. 1https://doi.org/10.1039/C5PY01601Kinfo:eu-repo/grantAgreement/EC/H2020/634928(c) Lagunas, A. et al., 2016info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1210712026-05-27T06:46:51Z
dc.title.none.fl_str_mv Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
title Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
spellingShingle Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
Lagunas, Anna
Copolímers
Nanoestructures
Nanotecnologia
Copolymers
Nanostructures
Nanotechnology
title_short Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
title_full Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
title_fullStr Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
title_full_unstemmed Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
title_sort Synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer containing biotin for selective protein nanopatterning
dc.creator.none.fl_str_mv Lagunas, Anna
Sasso, B.
Tesson, N.
Cantos, Carme
Martínez Fraiz, Elena
Samitier i Martí, Josep
author Lagunas, Anna
author_facet Lagunas, Anna
Sasso, B.
Tesson, N.
Cantos, Carme
Martínez Fraiz, Elena
Samitier i Martí, Josep
author_role author
author2 Sasso, B.
Tesson, N.
Cantos, Carme
Martínez Fraiz, Elena
Samitier i Martí, Josep
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Copolímers
Nanoestructures
Nanotecnologia
Copolymers
Nanostructures
Nanotechnology
topic Copolímers
Nanoestructures
Nanotecnologia
Copolymers
Nanostructures
Nanotechnology
description Protein patterning is of interest in high-throughput screening. Due to an increase in demand for further miniaturization of protein assays, block copolymers (BCPs) that can undergo large-area phase separation into nanometer-size domains have attracted great attention as substrates for protein nanopatterning. Here we report the synthesis of a polymethyl(methacrylate)-polystyrene-based diblock copolymer which, once spin-coated, is capable of self-segregating into cylindrical polystyrene (PS) domains. In this copolymer, the PS block was modified to introduce biotin below 10% molar in order to achieve molecular recognition of streptavidin. The PMMA matrix used to introduce poly(ethylene glycol) enabled us to obtain an antifouling environment that prevents unspecific protein adsorption outside the domains. The use of the biotin-streptavidin pair in this BCP makes it suitable for nanopatterning of other biotinylated proteins of interest for the purposes of cell biology, biosensors, and tissue engineering.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/121071
url https://hdl.handle.net/2445/121071
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1039/C5PY01601K
Polymer Chemistry, 2016, vol. 7, num. 1
https://doi.org/10.1039/C5PY01601K
info:eu-repo/grantAgreement/EC/H2020/634928
dc.rights.none.fl_str_mv (c) Lagunas, A. et al., 2016
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Lagunas, A. et al., 2016
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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