Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs

Background: Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, including energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content (mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet,...

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Detalles Bibliográficos
Autores: Llavanera, Marc, Mateo Otero, Yentel, Viñolas Vergés, Estel, Bonet, Sergi, Yeste Oliveras, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/24294
Acceso en línea:http://hdl.handle.net/10256/24294
Access Level:acceso abierto
Palabra clave:Mitocondris
ADN mitocondrial
Fecunditat
Mitochondria
Mitochondrial DNA
Fertility
Porcs -- Fecunditat
Swine -- Fertility
Semen -- Fisiologia
Semen -- Physiology
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oai_identifier_str oai:recercat.cat:10256/24294
network_acronym_str ES
network_name_str España
repository_id_str
spelling Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigsLlavanera, MarcMateo Otero, YentelViñolas Vergés, EstelBonet, SergiYeste Oliveras, MarcMitocondrisADN mitocondrialFecunditatMitochondriaMitochondrial DNAFertilityPorcs -- FecunditatSwine -- FertilitySemen -- FisiologiaSemen -- PhysiologyBackground: Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, including energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content (mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently interrogated. Results: First, the qPCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtDNAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater mtDNAc. Conclusions: These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fertility in livestock2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewed9 p.application/pdfhttp://hdl.handle.net/10256/24294Journal of Animal Science and Biotechnology, 2024, vol. 15, art. núm. 10, p. 1-9Articles publicats (D-B)Llavanera, Marc Mateo Otero, Yentel Viñolas Vergés, Estel Bonet, Sergi Yeste Oliveras, Marc 2024 Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs Journal Of Animal Science And Biotechnology 15 Art. num. 10 1 9reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s40104-023-00988-0info:eu-repo/semantics/altIdentifier/eissn/2049-1891Reconeixement 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10256/242942026-05-29T05:05:01Z
dc.title.none.fl_str_mv Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
title Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
spellingShingle Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
Llavanera, Marc
Mitocondris
ADN mitocondrial
Fecunditat
Mitochondria
Mitochondrial DNA
Fertility
Porcs -- Fecunditat
Swine -- Fertility
Semen -- Fisiologia
Semen -- Physiology
title_short Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
title_full Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
title_fullStr Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
title_full_unstemmed Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
title_sort Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
dc.creator.none.fl_str_mv Llavanera, Marc
Mateo Otero, Yentel
Viñolas Vergés, Estel
Bonet, Sergi
Yeste Oliveras, Marc
author Llavanera, Marc
author_facet Llavanera, Marc
Mateo Otero, Yentel
Viñolas Vergés, Estel
Bonet, Sergi
Yeste Oliveras, Marc
author_role author
author2 Mateo Otero, Yentel
Viñolas Vergés, Estel
Bonet, Sergi
Yeste Oliveras, Marc
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mitocondris
ADN mitocondrial
Fecunditat
Mitochondria
Mitochondrial DNA
Fertility
Porcs -- Fecunditat
Swine -- Fertility
Semen -- Fisiologia
Semen -- Physiology
topic Mitocondris
ADN mitocondrial
Fecunditat
Mitochondria
Mitochondrial DNA
Fertility
Porcs -- Fecunditat
Swine -- Fertility
Semen -- Fisiologia
Semen -- Physiology
description Background: Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, including energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content (mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently interrogated. Results: First, the qPCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtDNAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater mtDNAc. Conclusions: These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fertility in livestock
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/24294
url http://hdl.handle.net/10256/24294
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s40104-023-00988-0
info:eu-repo/semantics/altIdentifier/eissn/2049-1891
dc.rights.none.fl_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Reconeixement 4.0 Internacional
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
dc.source.none.fl_str_mv Journal of Animal Science and Biotechnology, 2024, vol. 15, art. núm. 10, p. 1-9
Articles publicats (D-B)
Llavanera, Marc Mateo Otero, Yentel Viñolas Vergés, Estel Bonet, Sergi Yeste Oliveras, Marc 2024 Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs Journal Of Animal Science And Biotechnology 15 Art. num. 10 1 9
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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