Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas

Knowledge of pathogen epidemiological dynamics and habitat ecological features is essential for wildlife population and health monitoring and management. Toxoplasma gondii and Neospora caninum are two broadly distributed multi-host parasites that affect both wild and domestic animals and, in the cas...

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Autores: Cano-Manuel, Alejandro, Enrique Granados, José, Álvarez-García, Gema, Huertas-López, Ana, Diezma-Díaz, Carlos, Cano-Manuel, Francisco Javier, Ortega-Mora, Luis Miguel, Fandos, Paulino, Mentaberre García, Gregorio, López-Olvera, Jorge Ramón, Martínez-Carrasco, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/469804
Acceso en línea:https://doi.org/10.1016/j.rvsc.2025.105530
https://hdl.handle.net/10459.1/469804
Access Level:acceso abierto
Palabra clave:ELISA
Eco-epidemiology
Iberian ibex
Neospora caninum
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oai_identifier_str oai:recercat.cat:10459.1/469804
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repository_id_str
dc.title.none.fl_str_mv Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
title Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
spellingShingle Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
Cano-Manuel, Alejandro
ELISA
Eco-epidemiology
Iberian ibex
Neospora caninum
title_short Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
title_full Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
title_fullStr Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
title_full_unstemmed Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
title_sort Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas
dc.creator.none.fl_str_mv Cano-Manuel, Alejandro
Enrique Granados, José
Álvarez-García, Gema
Huertas-López, Ana
Diezma-Díaz, Carlos
Cano-Manuel, Francisco Javier
Ortega-Mora, Luis Miguel
Fandos, Paulino
Mentaberre García, Gregorio
López-Olvera, Jorge Ramón
Martínez-Carrasco, Carlos
author Cano-Manuel, Alejandro
author_facet Cano-Manuel, Alejandro
Enrique Granados, José
Álvarez-García, Gema
Huertas-López, Ana
Diezma-Díaz, Carlos
Cano-Manuel, Francisco Javier
Ortega-Mora, Luis Miguel
Fandos, Paulino
Mentaberre García, Gregorio
López-Olvera, Jorge Ramón
Martínez-Carrasco, Carlos
author_role author
author2 Enrique Granados, José
Álvarez-García, Gema
Huertas-López, Ana
Diezma-Díaz, Carlos
Cano-Manuel, Francisco Javier
Ortega-Mora, Luis Miguel
Fandos, Paulino
Mentaberre García, Gregorio
López-Olvera, Jorge Ramón
Martínez-Carrasco, Carlos
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ELISA
Eco-epidemiology
Iberian ibex
Neospora caninum
topic ELISA
Eco-epidemiology
Iberian ibex
Neospora caninum
description Knowledge of pathogen epidemiological dynamics and habitat ecological features is essential for wildlife population and health monitoring and management. Toxoplasma gondii and Neospora caninum are two broadly distributed multi-host parasites that affect both wild and domestic animals and, in the case of T. gondii, cause zoonosis. This study reports the seroprevalence of both parasites in Iberian ibex (Capra pyrenaica), a mountain wild ruminant native to the Iberian Peninsula, from the Natural Space of Sierra Nevada (NSSN) in southeastern Spain. Serum from 146 Iberian ibexes were analysed using two in-house ELISA techniques. The positive and doubtful sera were further checked by Western Blot (WB). Seventeen ibexes (11.6 %; 95 % confidence interval 6.4-16.7) were positive for T. gondii and seven (4.8 %; 95 % confidence interval 1.3-8.2) for N. caninum. However, no sera were positive to T. gondii nor to N. caninum by WB. Using at least two different serological techniques is recommended when they are not validated for the target host species. The NSSN is a hypoendemic area for T. gondii and N. caninum, probably determined by the reduced abundance and restricted distribution of their definitive hosts. This would explain the hypoendemic situation in the NSSN and the lack of specific antibodies against these two parasites in the Iberian ibex population. This eco-epidemiological scenario can be challenged by climate and anthropogenic changes, recommending long-term monitoring Iberian ibex population and health, both as a conservation measure for the species and as an indicator of the potential impact of global change on high mountain ecosystems.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.rvsc.2025.105530
https://hdl.handle.net/10459.1/469804
https://hdl.handle.net/10459.1/469804
url https://doi.org/10.1016/j.rvsc.2025.105530
https://hdl.handle.net/10459.1/469804
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//CGL2012-40043-C02-01
info:eu-repo/grantAgreement/MINECO//CGL2012-40043-C02-02
info:eu-repo/grantAgreement/AEI//CGL2016-80543-P
Reproducció del document publicat a https://doi.org/10.1016/j.rvsc.2025.105530
Research in Veterinary Science, 2025, vol. 184, núm. 105530, p. 1-7
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Cano-Manuel et al., 2025
Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv cc-by-nc-nd (c) Cano-Manuel et al., 2025
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areasCano-Manuel, AlejandroEnrique Granados, JoséÁlvarez-García, GemaHuertas-López, AnaDiezma-Díaz, CarlosCano-Manuel, Francisco JavierOrtega-Mora, Luis MiguelFandos, PaulinoMentaberre García, GregorioLópez-Olvera, Jorge RamónMartínez-Carrasco, CarlosELISAEco-epidemiologyIberian ibexNeospora caninumKnowledge of pathogen epidemiological dynamics and habitat ecological features is essential for wildlife population and health monitoring and management. Toxoplasma gondii and Neospora caninum are two broadly distributed multi-host parasites that affect both wild and domestic animals and, in the case of T. gondii, cause zoonosis. This study reports the seroprevalence of both parasites in Iberian ibex (Capra pyrenaica), a mountain wild ruminant native to the Iberian Peninsula, from the Natural Space of Sierra Nevada (NSSN) in southeastern Spain. Serum from 146 Iberian ibexes were analysed using two in-house ELISA techniques. The positive and doubtful sera were further checked by Western Blot (WB). Seventeen ibexes (11.6 %; 95 % confidence interval 6.4-16.7) were positive for T. gondii and seven (4.8 %; 95 % confidence interval 1.3-8.2) for N. caninum. However, no sera were positive to T. gondii nor to N. caninum by WB. Using at least two different serological techniques is recommended when they are not validated for the target host species. The NSSN is a hypoendemic area for T. gondii and N. caninum, probably determined by the reduced abundance and restricted distribution of their definitive hosts. This would explain the hypoendemic situation in the NSSN and the lack of specific antibodies against these two parasites in the Iberian ibex population. This eco-epidemiological scenario can be challenged by climate and anthropogenic changes, recommending long-term monitoring Iberian ibex population and health, both as a conservation measure for the species and as an indicator of the potential impact of global change on high mountain ecosystems.This study was performed within the context of the Educational Collaboration Agreement signed by the University of Murcia and the Consejería de Medio Ambiente y Ordenación del Territorio of the Junta de Andalucía. The authors are grateful by the support received by the research group RNM-118 from the Plan Andaluz de Investigación. The study benefitted from funding of the Spanish Ministerio de Economía y Competitividad through the grants CGL2012-40043-C02-01, CGL2012- 40043-C02-02, and CGL2016-80543-P. Ana Huertas-López was supported by a postdoctoral contract Margarita Salas (University of Murcia) from the Program of Requalification of the Spanish University System (Spanish Ministry of Universities) financed by the European Union — NextGenerationEU [grant number R-1593/2022]. Special thanks are also due to the park rangers and fieldworkers of the NSSN and, particularly, to José López Pérez, Isidro Puga González, Elías Martínez Ortiz, Apolo Sánchez Lao and Antonio Rodríguez Dueñas for their professional and personal involvement in the study.Elsevier2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1016/j.rvsc.2025.105530https://hdl.handle.net/10459.1/469804https://hdl.handle.net/10459.1/469804reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MINECO//CGL2012-40043-C02-01info:eu-repo/grantAgreement/MINECO//CGL2012-40043-C02-02info:eu-repo/grantAgreement/AEI//CGL2016-80543-PReproducció del document publicat a https://doi.org/10.1016/j.rvsc.2025.105530Research in Veterinary Science, 2025, vol. 184, núm. 105530, p. 1-7cc-by-nc-nd (c) Cano-Manuel et al., 2025Attribution-NonCommercial-NoDerivatives 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:recercat.cat:10459.1/4698042026-05-29T05:05:01Z
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