Development and Validation of a New High-Performance Liquid Chromatography Method for the Simultaneous Quantification of Coenzyme Q10, Phosphatidylserine, and Vitamin C from a Cutting-Edge Liposomal Vehiculization

A high-performance liquid chromatography (HPLC) method was developed to simultaneously quantify three widely used active substances such as coenzyme Q10, phosphatidylserine, and vitamin C. This new method optimizes current timing and costs in the analyses of these three active substances. Additional...

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Detalles Bibliográficos
Autores: Ruiz García, Marta, Pérez Lozano, Pilar|||0000-0001-6899-066X, Mercadé Frutós, Débora, Nardi Ricart, Anna, Suñé Pou, Marc, Cano-Sarabia, Mary|||0000-0003-4254-8157, García Jimeno, Sonia|||0000-0003-3821-2714, Suñé-Negre, Josep Maria, Maspoch Comamala, Daniel|||0000-0003-1325-9161, García Montoya, Encarna
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:222259
Acceso en línea:https://ddd.uab.cat/record/222259
https://dx.doi.org/urn:doi:10.1021/acsomega.9b02456
Access Level:acceso abierto
Palabra clave:Nutrition
High-performance liquid chromatography
Solvents
Organic compounds
Descripción
Sumario:A high-performance liquid chromatography (HPLC) method was developed to simultaneously quantify three widely used active substances such as coenzyme Q10, phosphatidylserine, and vitamin C. This new method optimizes current timing and costs in the analyses of these three active substances. Additionally, since the analyzed compounds were encapsulated on a cutting-edge liposomal formulation, further processing was necessary to be developed prior to HPLC analyses. The technique was studied and adequately validated in accordance with the guidelines of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) regarding selectivity, linearity, accuracy, precision, and robustness. After data treatment of results, linear regressions for all active substances showed an optimal linearity with a correlation coefficient of >0.999 in the concentration range between 70 to 130% of the liposomal formulation and less than a 3% relative standard deviation (RSD) in accuracy and precision.

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